Journal of Analytical Chemistry

, Volume 56, Issue 4, pp 359–363 | Cite as

Determination of Volatile N-Nitrosamines in Food by High-Performance Liquid Chromatography with Fluorescence Detection

  • N. V. Komarova
  • A. A. Velikanov


A procedure is proposed for the determination of the mass fraction of volatile N-nitrosamines in food by high-performance liquid chromatography with fluorescence detection. A high sensitivity and selectivity of determination is attained by the precolumn operation of obtaining derivatives secondary amines, which were obtained by the denitrosation of initial nitrosamines with 5-dimethylamino-1-naphthalenesulfonyl chloride (dansyl chloride). A mixture of concentrated hydrobromic acid and glacial acetic acid was proposed as the reducing agent for the denitrosation reaction.


Acetic Chloride Chromatography Analytical Chemistry Acetic Acid 
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Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • N. V. Komarova
    • 1
  • A. A. Velikanov
    • 1
  1. 1.Analytical Instrument-Making Research and Manufacturing Company LyumeksSt. PetersburgRussia

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