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Determination of Vitamins E and D3 in Some Preparations by High-Performance Liquid Chromatography with Indirect Spectrophotometric Detection

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Abstract

The behavior of vitamins E and D3 was studied under the conditions of reversed-phase high-performance liquid chromatography with the use of unmodified and modified mobile phases. Acetonitrile modified with chlorophyll (a + b) (Chl) and tetraphenylporfyrin (H2TPP) was selected for the indirect spectrophotometric detection of vitamins. It was demonstrated that detection limits are 2.3 μg (8.33 × 10–6 M H2TPP, λ= 404 nm) and 3.5 ng (8.6 × 10–6 M Chl, λ = 430 nm) for vitamin E and 0.12 μg (3.0 × 10–7 M H2TPP, λ = 420 nm) for vitamin D3 . Pharmaceutical preparations containing vitamins E and D3 were analyzed.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia

    E. E. Lazareva, G. D. Brykina & O. A. Shpigun

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  1. E. E. Lazareva
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  2. G. D. Brykina
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  3. O. A. Shpigun
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Lazareva, E.E., Brykina, G.D. & Shpigun, O.A. Determination of Vitamins E and D3 in Some Preparations by High-Performance Liquid Chromatography with Indirect Spectrophotometric Detection. Journal of Analytical Chemistry 57, 616–619 (2002). https://doi.org/10.1023/A:1016282102216

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