Molecular and Cellular Biochemistry

, Volume 233, Issue 1–2, pp 1–8 | Cite as

N-cadherin involvement in the heterotypic adherence of malignant T-cells to epithelia

  • Irwan T. Makagiansar
  • Helena Yusuf-Makagiansar
  • Atsutoshi Ikesue
  • Anna M. Calcagno
  • Joseph S. Murray
  • Teruna J. Siahaan


N-cadherin, a cell adhesion molecule normally found in neural cell tissue, has been found recently to be expressed on the surface of malignant T-cells. The function of N-cadherin on these cells remains unclear. Heterotypic assays between Molt-3 T lymphoblastic leukemia cells and Caco-2 epithelial monolayers were examined under different conditions to assess the functional role of N-cadherin. The results indicate that adherence of Molt-3 cells to Caco-2 monolayers was reduced significantly following pretreatment of Molt-3 cells with 100 μM of an N-cadherin-derived antagonist decapeptide. In contrast, pretreatment of Molt-3 cells with an anti-N-cadherin antibody raised against the first 20 amino acids of N-cadherin sequence led to a surprisingly marked enhancement of Molt-3 cell adherence to Caco-2 monolayers. In addition, the presence of anti-N-cadherin antibody neutralized the inhibitory effect of anti-ICAM-1 on Molt-3 adhesion to Caco-2 monolayers. This novel finding demonstrates that external stimulus through the N-cadherin amino terminus can modulate adhesion of malignant T-cells to epithelia and may promote their ability to invade or metastasize to inflammatory sites.

malignant T-cells Molt-3 Caco-2 N-cadherin β-catenin anti-N-cadherin antibody anti-ICAM-1 antibody 


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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Irwan T. Makagiansar
  • Helena Yusuf-Makagiansar
  • Atsutoshi Ikesue
  • Anna M. Calcagno
  • Joseph S. Murray
  • Teruna J. Siahaan

There are no affiliations available

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