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Dissimilar invasive and metastatic behavior of vincristine and doxorubicin-resistant cell lines derived from a murine T cell lymphoid leukemia

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Abstract

Multidrug resistance (MDR) lines from a murine T-cell lymphoid leukemia were selected in increasing vincristine (VCR) or doxorubicin (DOX) concentrations. Surface markers were determined by flow cytometry in both resistant (LBR-V160 and LBR-D160) and sensitive (LBR-) cell lines. Results obtained revealed similar expression of CD25, CD24, CD8, CD4, C18 and CD44, while differences in binding to hyaluronic acid (HA) were found. LBR- and LBR-D160 bound to HA only after phorbol ester (PMA) activation, while LBR-V160 failed to bind HA even after PMA treatment. Histopathological analysis disclosed that LBR-V160 was less invasive than LBR- and LBR-D160 cell lines. In vitro growth of cell lines analyzed by sulforhodamine-B uptake showed that doubling time for the three lines was 10.24 h (LBR-), 16.75 h (LBR-V160) and 16.29 h (LBR-D160). Mortality rate was determined after i.p. injection of 104 cells. Mice inoculated with LBR- died at 23 (± 2.11) days, while those inoculated with LBR-V160 or LBR-D160 died at 41 (± 9.53) or 41 (± 4.96) days, respectively. Our results demonstrated that leukemic murine T cells cultured in the long-term presence of VCR or DOX not only presented changes in the resistance phenotype but also variations in their growth and metastatic pattern.

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Authors and Affiliations

  1. Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), IDEHU-CONICET, Argentina

    Eloisi C. Lopes, Glenda Ernst, Paula Aulicino, Mariana García, Elida Alvarez & Silvia E. Hajos

  2. Departamento de Patología, Centro de Estudios Oncológicos (CEO-FM), Academia Nacional de Medicina, Buenos Aires, Argentina

    Silvia Vanzulli

Authors
  1. Eloisi C. Lopes
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  2. Glenda Ernst
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  3. Paula Aulicino
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  4. Silvia Vanzulli
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  5. Mariana García
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  6. Elida Alvarez
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  7. Silvia E. Hajos
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Correspondence to Eloisi C. Lopes.

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Lopes, E.C., Ernst, G., Aulicino, P. et al. Dissimilar invasive and metastatic behavior of vincristine and doxorubicin-resistant cell lines derived from a murine T cell lymphoid leukemia. Clin Exp Metastasis 19, 283–290 (2002). https://doi.org/10.1023/A:1015548706673

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  • DOI: https://doi.org/10.1023/A:1015548706673

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