Abstract
Plants of sunflower (Helianthus annuus L. cv Giza2) were salt-stressed with a combination of NaCl and CaCl2 inconcentrations having different osmotic potentials (ψs from 0 to −1.0MPa) and were treated with 5 and 10mg L−1 of thiamin either sprayed on the shoot orapplied to the root. The membranes of leaf discs from salt-stressed plantsappeared to be less stable (more injured) under heat(51 °C) and drought (40% polyethylene glycol6000) stresses than control plants. Salinity slowed the rate of growth (lengthand dry mass production), lowered leaf relative water content (RWC) and leafandroot water potential (ψw), decreased the contents of chlorophyll (Chl),soluble sugars (SS) and the K+/Na+ ratio butenhanced total free amino acids (TAA), Na+,Ca2+and Cl− accumulation in the shoot and root system. Root orshoot application of thiamin reduced membrane injury by either heat ordehydration stress, lowered leaf ψw, improved uptake of K+,and increased leaf RWC, Chl, SS, TAA contents and dry mass production. Theeffects of salinity (ψs), thiamin (Thi.) and their interaction(ψs × Thi) on the parameters tested were significant.Salinity was dominant (as indicated by η2 values) in affectingthe contents of Ca2+, Cl−, TAA and membranestability to heat and leaf ψw. The role of thiamin was dominant forNa+, K+ and SS contents and the contribution ofinteraction was dominant for growth parameters, Chl. and root ψw.
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Abdel-Krim L. and Dorothy M.L. 1995. Contribution of carbohydrates and other solutes to osmotic adjustment in wheat leaves under water stress. J. Plant Physiol. 14: 363–366.
Asano Y., Katsumoto H., Inokuma C., Kaneko S., Iro Y. and Fujiie A. 1996. Cytokinin and thiamin requirements and stimulative effects of riboflavin and _-ketoglutaric acid on embryogenic callus induction from the seeds of Zoysia japonica Steud. J. Plant Physiol. 149: 413–417.
Blum A. and Ebercon A. 1981. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop. Sci. 21: 43–47.
Bohra J.S. and Dörffling K. 1993. Potassium nutrition of rice (Oryza sativa L.) varieties under NaCl salinity. Plant and Soil 152: 299–303.
Bonner J. 1940. On the growth factor requirements of isolated roots. Amer. J. Bot. 24: 692–701.
Bonner J. 1942. Transport of thiamin in the tomato plant. Amer. J. Bot. 29: 136–142.
Botella M.A., Martinez V., Pardines J. and Cerda A. 1997. Salinity induced potassium deficiency in maize plants. J. Plant Physiol 150: 200–205.
Buysse J. and Merckx R. 1993. An improved colorimetric method to quantify sugar content of plant tissue. J. Exp. Bot. 44: 1627–1629.
Chow W.S., Ball M.C. and Anderson J.M. 1990. Growth and photosynthetic responses of spinach to salinity. Implications of K+ nutrition for salt tolerance. Aust. J. Plant Physiol. 17: 563–578.
Crowe J.H. and Crowe L.M. 1992. Membrane integrity in anhydrobiotic organisms. Toward a mechanism for stabilizing dry cells. In: Somer G.N., Osmond C.B. and Bolis C.L. (eds), Water and Plant Life. Springer-Verlag, Berlin, pp. 87–103.
Cutler J.M., Steponkus P.L., Wach M.J. and Shahan K.W. 1980. Dynamic aspects and enhancement of leaf elongation in rice. Plant Physiol. 66: 147–152.
Davenport R.J., Reid R.J. and Smith F.A. 1997. Sodium-calcium interactions in two wheat differing in salinity tolerance. Physiol. Plant 99: 325–327.
Downs R.J. and Hellmers H. 1975. Environment and Experimental Control of Plant Growth. Academic Press London, New York, San Francisco, 145 p.
Elmsheuser H., Neumann K.H. and Schuster W. 1978. Investigation on the effect of different vitamins and some phytohormones on growth and development of tissue cultures of some Brassica species. Angew. Botanik. 52: 193–201.
Erdei L. and Taleisnik E. 1993. Changes in water relation parameters under osmotic and salt stresses in maize and sorghum. Physiol. Plant 89: 381–387.
Erdei L., Trivedi S., Takeda K. and Matsumoto H. 1990. Effects of osmotic and salt stresses on the accumulation of polyamines in leaf segments from wheat varieties differing in salt and drought tolerance. J. Plant Physiol. 137: 165–168.
Epstein E. 1961. The essential role of calcium in selective cation transport by plant cells. Plant Physiol. 36: 437–444.
Gadallah M.A.A. 1995. Effect of waterlogging and kinetin on the stability of leaf membranes, leaf osmotic potential, soluble carbon and nitrogen compounds and chlorophyll content of Ricinus plants. Phyton. 35: 199–208.
Gadallah M.A.A. 1996. Abscisic acid, temperature and salinity interactions on growth and some mineral elements in Carthamus plants. Plant Growth Reg. 20: 225–236.
Gadallah M.A.A. 1999. Effects of proline and glycinebetaine on Vicia faba responses to salt stress. Biol. Plant. 42: 63–74.
Gadallah M.A.A. and Ramadan T. 1997. Effects of zinc and salinity on growth and anatomical structure of Carthamus tinctorius L. Biol. Plant. 39: 411–418.
Greenway H. and Munns R. 1980. Mechanisms of salt tolerance in nonhalophytes. Annu. Rev. Plant Physiol. 31: 149–190.
Johnson C.M. and Ulrich A. 1959. Analytical methods for use in plant analysis. In:, U.S. Department of Agriculture, California University. Agriculture Inform. Bull., p. 766.
Lagerwerff J.V. and Eagle H.E. 1961. Osmotic and specific effects of excess salts on beans. Plant Physiol. 36: 472–477.
Lee Y.P. and Takahashi T. 1966. An improved colorimetric determination of amino acids with the use of ninhydrin. Analy. Biochem. 14: 71–77.
Leopold A.C. and Willing R.P. 1984. Evidence for toxicity effects of salt on membranes. In: Staples, R.C. and Toenniessen, G.J. (eds), Salinity Tolerance in Plants: Strategies for Crop Improvement. John Wiley and Sons, New York, pp. 67–75.
Läuchli A. and Epstein E. 1972. Transport of potassium and rapidium in plant roots. The significance of calcium. Plant Physiol. 45: 639–641.
Mateikene I.K., Bandzhyulene R.S., Ozheraitene M.V. and Bluzmanas P.I. 1988. Uptake and distribution of 14C-thiamin in barley caryopses and plants. Soviet Plant Physiol. 35: 881–889.
Mozafar A. and Oertli J.J. 1992. Uptake and transport of thiamin (vitamin B1) by barley and soybean. J. Plant Physiol. 159: 436–442.
Mozafar A. and Oertli J.J. 1993. Thiamin (vitamin B1): Translocation and metabolism by soybean seedling. J. Plant Physiol. 142: 438–445.
Oertli J.J. 1987. Exogenous application of vitamins as regulators for growth development of plants - a review. Z. Pflanzenernahr. Bodenk. 150: 375–391.
Ostle B. 1963. Statistics in Research. Iowa State University Press, 585 p.
Premachandra G.S., Saneoka H., Fujita K. and Ogata S. 1992. Osmotic adjustment and stomatal response to water deficits in maize. J. Exp. Bot. 43: 1451–1456.
Proebsting W.M., Maggard S.P. and Guo W.W. 1990. The relationship of thiamin to the alt locus of Pisum sativum L. J. Plant Physiol. 136: 231–235.
Sabater B. and Rodriquez M.T. 1978. Control of chlorophyll degradation in detached leaves of barley and oat through effect of kinetin on chlorophyllase levels. Physiol. Plant. 43: 274–276.
Schulte-Altedorneburg M., Marx S., Schneider-Poetsch Hj A.W. and Willenbrink J. 1989. Quantitative determination and distribution of free and conjugated ABA in sugar beet plants. J. Plant Physiol. 135: 52–56.
Seemann J. and Critchley C. 1985. Effect of salt stress on growth, ion content, stomatal behaviour and photosynthetic capacity of a salt sensitive species, Phaseolus vulgaris L. Planta 164: 151–161.
Shimizu M., Mitsunaga T., Inaba K., Yoshida T. and Iwashima A. 1990. Accumulation of thiamin and thiamin-binding protein during development of rice seed. J. Plant Physiol. 137: 123–124.
Todd G.W. and Basler E. 1965. Fate of various protoplasmic constituents in droughted wheat plants. Phyton 22: 79–85.
Weatherley P.E. and Barrs C. 1962. A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust. J. Biol. Sci. 15: 413–428.
Yeo A.R., Caporn S.J.M. and Flowers T.J. 1985. The effect of salinity upon photosynthesis in rice (Oryza sativa L.): gas exchange by individual leaves in relation to their salt content. J. Exp. Bot. 36: 1240–1248.
Yeo A.R., Yeo M.E. and Flowers T.J. 1987. The contribution of an apoplastic pathway to sodium uptake by rice roots in saline conditions. J. Exp. Bot. 38: 1141–1163.
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Sayed, S., Gadallah, M. Effects of shoot and root application of thiamin on salt-stressed sunflower plants. Plant Growth Regulation 36, 71–80 (2002). https://doi.org/10.1023/A:1014784831387
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DOI: https://doi.org/10.1023/A:1014784831387