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Chromosome Research

, Volume 10, Issue 1, pp 49–54 | Cite as

In-Situ Comparative Mapping (ISCM) of Glu-1 Loci in Triticum and Hordeum

  • A. Cabrera
  • A. Martín
  • F. Barro
Article

Abstract

The ability to detect small low- or single-copy DNA sequences by fluorescence in-situ hybridization (FISH) is an important step towards physical mapping of plant genomes. In this study, the FISH technique was used to physically map the Glu-1 loci controlling high-molecular weight (HMW) glutenin in common wheat (Triticum aestivum cv. 'Chinese Spring') and tritordeum (am amphiploid between T. turgidum cv. durum and Hordeum chilense). The probe used was the single-copy Glu-D1-1d gene coding the 1Dx5 HMW glutenin subunit. Three loci were mapped on chromosomes of wheat homoeologous group 1 (arm 1AL, 1BL and 1DL). The Glu-1 loci were mapped (fraction of the distance from the centromere) at positions 0.76 ± 0.01, 0.69 ± 0.01 and 0.76 ± 0.01, on arms 1AL, 1BL and 1DL, respectively. The Glu-1 loci were also mapped on chromosomes of homoeologous group 1 of tritordeum at positions 0.75 ± 0.01, 0.70 ± 0.01 and 0.60 ± 0.01, on arms 1AL, 1BL and 1HchL, respectively. Chromosomes with positive signals were identified by reprobing chromosome preparations using both the GAA-satellite and pAs1 sequences simultaneously. The application of the FISH technique to study homoeology among different genomes is discussed.

FISH glutenin Hordeum in-situ comparative mapping (ISCM) Triticum 

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • A. Cabrera
    • 1
  • A. Martín
    • 2
  • F. Barro
    • 2
  1. 1.Department of Genetics, ETSIAMUniversity of CórdobaCórdobaSpain
  2. 2.Department of Agronomy and Plant BreedingInstitute of Sustainable Agriculture, CSICCórdobaSpain

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