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Chromosome Research

, Volume 10, Issue 1, pp 45–48 | Cite as

Meiotic Chromosomes as Templates for Microdissection

  • Allen T. Christian
  • Melissa S. Pattee
  • Francesco Marchetti
Article

Abstract

As templates for chromosome microdissection, meiotic cells offer several advantages over mitotic cells. The pairing of homologous chromosomes at the metaphse plate of the first meiotic division allows the simultaneous isolation of two copies of the same chromosome, and the sex chromosomes are easy to identify in male meiotic cells. We report on a method for making fluorescence in-situ hybridization (FISH) probes from dissected meiotic chromosomes.

chromosome FISH meiosis microdissection mouse 

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References

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Copyright information

© Kluwer Academic Publishers 2002

Authors and Affiliations

  • Allen T. Christian
    • 1
  • Melissa S. Pattee
    • 1
  • Francesco Marchetti
    • 1
  1. 1.Biology and Biotechnology Research ProgramLawrence Livermore National LaboratoryLivermoreUSA

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