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Ca2+ and Cu2+ supplementation augments vancomycin production by Amycolatopsis orientalis

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Abstract

Supplementation with CaCl2⋅2H2O (40 mg l−1) or CuSO4⋅5H2O (10 mg l−1) improved vancomycin production by Amycolatopsis orientalis by 12 and 11%, respectively; used in combination, they acted synergistically. Ca2+ decreased the intracellular concentration of vancomycin 36%, whereas Cu2+ increased the intracellular activity of TDP-glucose:aglycosylvancomycin glucosyltransferase 3 times more than control. Ca2+ probably works by altering the permeability of cells to vancomycin, whereas Cu2+ increases the activity of an enzyme responsible for vancomycin biosynthesis.

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Authors and Affiliations

  1. BioNgene Co., Ltd., Chongro-Ku, Seoul, 110-521, Korea

    Hyung-Moo Jung, Sang-Yong Kim & Jung-Kul Lee

  2. Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, 89 Wangsan-ri, Mohyun, Yongin-si, Kyounggi-do, 449-791, Korea

    Hyung-Hwan Hyun

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  1. Hyung-Moo Jung
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  2. Sang-Yong Kim
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  3. Hyung-Hwan Hyun
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  4. Jung-Kul Lee
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Jung, HM., Kim, SY., Hyun, HH. et al. Ca2+ and Cu2+ supplementation augments vancomycin production by Amycolatopsis orientalis . Biotechnology Letters 24, 293–296 (2002). https://doi.org/10.1023/A:1014097506735

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  • DOI: https://doi.org/10.1023/A:1014097506735

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