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Opposite Effects of Mn(III) and Fe(III) Forms of meso-Tetrakis(4-N-methyl pyridiniumyl) Porphyrins on Isolated Rat Liver Mitochondria

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Abstract

The relevance of porphyrins as therapeutic drugs targeted to mitochondria has been widely recognized. In this work, we studied the action of meso-tetrakis porphyrins (TMPyP) on respiring rat liver mitochondria. Mn(III)TMPyP exerted a protective effect against lipid peroxidation induced by Fe(II) or the azo initiator 4,4′-azobis(4-cyanopentanoic acid) (ABCPA), which partition in the hydrophobic phospholipid moiety, and 2,2′-azobis(2-amidinepropane)dihydrochloride (ABAP), which partitions in the aqueous phase. In contrast, Fe(III)TMPyP itself induced an intense lipid peroxidation, accompanied by mitochondrial permeability transition. Both mesoporphyrins studied promoted a release of mitochondrial state-4 respiration, in the concentration range of 1.0–20 μM. Based on the relative effects of Mn(III)TMPyP against ABAP and ABCPA-induced lipid peroxidation, we believe that meso-tetrakis porphyrins must concentrate preferably at membrane–water interfaces.

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Authors and Affiliations

  1. Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, 13083-970, Campinas, SP, Brazil

    M. F. Nepomuceno & A. E. Vercesi

  2. Universidade de São Paulo, C. P. 780, 13560-970, São Carlos, SP, Brazil

    M. Tabak

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  1. M. F. Nepomuceno
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  2. M. Tabak
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  3. A. E. Vercesi
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Correspondence to A. E. Vercesi.

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Nepomuceno, M.F., Tabak, M. & Vercesi, A.E. Opposite Effects of Mn(III) and Fe(III) Forms of meso-Tetrakis(4-N-methyl pyridiniumyl) Porphyrins on Isolated Rat Liver Mitochondria. J Bioenerg Biomembr 34, 41–47 (2002). https://doi.org/10.1023/A:1013818719932

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