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Microsatellite-centromere mapping in the loach, Misgurnus anguillicaudatus

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Abstract

Primer sets for 15 polymorphic microsatellite loci were developed in the loach, Misgurnus anguillicaudatus (Cobitidae) by molecular cloning and sequencing techniques. Mendelian inheritance was confirmed for the 15 loci by examining the genotypic segregation produced with the primer sets in two full-sib families. The loci were mapped in relation to their centromere in four gynogenetic diploid lines, which were induced by inhibition of the second meiotic division after fertilization with genetically inert sperm. Microsatellite-centromere recombination rates ranged between 0.06 and 0.95 under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes. The success of mitotic gynogenesis, produced by suppression of the first cleavage, was verified by homozygosity at three diagnostic microsatellite loci that exhibited high gene-centromere meiotic recombination rates in the same family. The differences in heterozygosity levels observed with these markers were attributed to differences in the temporal application of heat shock following inert sperm activation.

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Author notes

  1. I. Nakayama

    Present address: Secretariat to Council for Science and Technology Policy, Cabinet Office, Government of Japan, 1-1-15 Nishishinbashi, Minatoku, Tokyo, 105-0003, Japan

Authors and Affiliations

  1. Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, 041-8611, Japan

    K. Morishima & K. Arai

  2. National Research Institute of Aquaculture, Tamaki, Mie, 519-0423, Japan

    I. Nakayama

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  1. K. Morishima
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  2. I. Nakayama
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  3. K. Arai
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Morishima, K., Nakayama, I. & Arai, K. Microsatellite-centromere mapping in the loach, Misgurnus anguillicaudatus . Genetica 111, 59–69 (2001). https://doi.org/10.1023/A:1013701128529

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