, Volume 122, Issue 1, pp 31–36 | Cite as

Screening wild plants of Capsicum annuum for resistance to pepper huasteco virus (PHV): Presence of viral DNA and differentiation among populations

  • Sergio Hernández-Verdugo
  • Ramón G. Guevara-González
  • Rafael F. Rivera-Bustamante
  • Ken Oyama


Plants collected in thirteen wild populations of Capsicum annuum from Northwest Mexico were tested for resistance to the pepper huasteco begomovirus (formerly subgroup III) (PHV) that is transmitted by the white fly Bemisia tabaci Genadius. Plants were inoculated using both grafting and biolistic methods. Presence of viral DNA was detected by dot blot hybridization and densitometry. Populations varied in their resistance to PHV. Plants of only two of the populations either did not develop disease symptoms or showed very light symptoms after inoculation. In some cases, symptoms appeared several days after inoculation. In plants of these populations viral DNA was detected by dot-blot hybridization assays but they appear to be a good source of resistance (symptomless) for use in breeding programmes.

biolistic inoculation Capsicum geminivirus graft-inoculation PHV resistance 


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Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Sergio Hernández-Verdugo
    • 1
  • Ramón G. Guevara-González
    • 2
  • Rafael F. Rivera-Bustamante
    • 2
  • Ken Oyama
    • 3
  1. 1.Facultad de AgronomíaUniversidad Autónoma de Sinaloa Instituto de Ecología, Universidad Nacional Autónoma de México (UNAM)México, D.FMéxico
  2. 2.Centro de Investigaciones de Estudios Avanzados (CINVESTAV-IPN), Unidad IrapuatoIrapuato, GuanajuatoMéxico
  3. 3.Instituto de EcologíaUniversidad Nacional Autónoma de México (UNAM)México, D.FMéxico

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