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Journal of Biomolecular NMR

, Volume 20, Issue 2, pp 173–176 | Cite as

A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules

  • Bernd Simon
  • Katia Zanier
  • Michael Sattler
Article

Abstract

A new TROSY relayed HCCH-COSY pulse sequence is introduced for correlating adenine H2 and H8 resonances in 13C-labeled RNA molecules. The pulse scheme provides substantial improvements in signal-to-noise compared to previously suggested experiments, and therefore will be suitable for NMR studies of larger RNA molecules. The experiment provides 13C chemical shifts for all carbon nuclei in the adenine base. This is advantageous for resolving spectral overlap in larger RNA molecules and provides a starting point for measuring additional parameters for these carbons in the adenine spin system.

adenine 13C-labeling HCCH-COSY RNA assignment TROSY 

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References

  1. Batey, R.T., Inada, M., Kujawinski, E., Puglisi, J.D. and Williamson, J.R. (1992) Nucleic Acids Res., 20, 4515-4523.CrossRefGoogle Scholar
  2. Brutscher, B., Boisbouvier, J., Pardi, A., Marion, D. and Simorre, J.-P. (1998) J. Am. Chem. Soc., 120, 11845-11851.CrossRefGoogle Scholar
  3. Farmer II, B.T., Müller, L., Nikonowicz, E.P. and Pardi, A. (1993) J. Am. Chem. Soc., 115, 11040-11041.CrossRefGoogle Scholar
  4. Fiala, R., Czernek, J. and Sklenar, V. (2000) J. Biomol. NMR, 16, 291-302.CrossRefGoogle Scholar
  5. Fiala, R., Jiang, F. and Patel, D.J. (1996) J. Am. Chem. Soc., 118, 689-690.CrossRefGoogle Scholar
  6. Geen, H. and Freeman, R. (1991) J. Magn. Reson., 93, 93-141.Google Scholar
  7. Heus, H.A., Wijmenga, S.S., van de Ven, F.J.M. and Hilbers, C.W. (1994) J. Am. Chem. Soc., 116, 4983-4984.CrossRefGoogle Scholar
  8. Ippel, J.H., Wijmenga, S.S., de Jong, R., Heus, H.A., Hilbers, C.W., de Vroom, E., van der Marel, G.A. and van Boom, J.H. (1996) Magn. Reson. Chem., 34, S156-S176.CrossRefGoogle Scholar
  9. Legault, P., Farmer II, B.T., Müller, L. and Pardi, A. (1994) J. Am. Chem. Soc., 116, 2203-2204.CrossRefGoogle Scholar
  10. Marino, J.P., Prestegard, J.H. and Crothers, D.M. (1994a) J. Am. Chem. Soc., 116, 2205-2206.CrossRefGoogle Scholar
  11. Marino, J.P., Schwalbe, H., Anklin, C., Bermel, W., Crothers, D.M. and Griesinger, C. (1994b) J. Am. Chem. Soc., 116, 6472-6473.CrossRefGoogle Scholar
  12. Meissner, A. and Sø rensen, O.W. (1999a) J. Magn. Reson., 139, 447-450.CrossRefADSGoogle Scholar
  13. Meissner, A. and Sø rensen, O.W. (1999b) J. Magn. Reson., 139, 439-442.CrossRefADSGoogle Scholar
  14. Nikonowicz, E.P., Sirr, A., Legault, P., Jucker, F.M., Baer, L.M. and Pardi, A. (1992) Nucleic Acids Res., 20, 4507-4513.CrossRefGoogle Scholar
  15. Pervushin, K., Riek, R., Wider, G. and Wüthrich, K. (1997) Proc. Natl. Acad. Sci. USA, 94, 12366-12371.CrossRefADSGoogle Scholar
  16. Pervushin, K., Riek, R., Wider, G. and Wüthrich, K. (1998) J. Am. Chem. Soc., 120, 6394-6400.CrossRefGoogle Scholar
  17. Ramachandran, R., Sich, C., Grune, M., Soskic, V. and Brown, L.R. (1996) J. Biomol. NMR, 7, 251-255.CrossRefGoogle Scholar
  18. Riek, R., Pervushin, K., Fernández, C., Kainosho, M. and Wüthrich, K. (2001) J. Am. Chem. Soc., 123, 658-664.CrossRefGoogle Scholar
  19. Salzmann, M., Pervushin, K., Wider, G., Senn, H. and Wüthrich, K. (1998) Proc. Natl. Acad. Sci. USA, 95, 13585-13590.CrossRefADSGoogle Scholar
  20. Salzmann, M., Pervushin, K., Wider, G., Senn, H. and Wüthrich, K. (2000) J. Am. Chem. Soc., 122, 7543-7548.CrossRefGoogle Scholar
  21. Sattler, M., Schleucher, J. and Griesinger, C. (1999) Prog. NMR Spectrosc., 34, 93-158.CrossRefGoogle Scholar
  22. Scott, L.G., Tolbert, T.J. and Williamson, J.R. (2000) Methods Enzymol., 317, 18-38.CrossRefGoogle Scholar
  23. Simorre, J.-P., Zimmermann, G.R., Müller, L. and Pardi, A. (1996) J. Biomol. NMR, 7, 153-156.CrossRefGoogle Scholar
  24. Sklenar, V., Dieckmann, T., Butcher, S.E. and Feigon, J. (1998) J. Magn. Reson., 130, 119-124.CrossRefADSGoogle Scholar
  25. Sklenar, V., Peterson, R.D., Rejante, M.R., Wang, E. and Feigon, J. (1992) J. Am. Chem. Soc., 115, 12181-12182.CrossRefGoogle Scholar
  26. Tate, S., Ono, A. and Kainosho, M. (1995) J. Magn. Reson., B106, 89-91.Google Scholar
  27. Varani, G., Aboul-ela, F. and Allain, F.H.-T. (1996) Progr. NMR Spectrosc., 29, 51-127.CrossRefGoogle Scholar
  28. Wijmenga, S. and van Buuren, B.N.M. (1998) Progr. NMR Spectrosc., 32, 287-387.CrossRefGoogle Scholar
  29. Wüthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley, New York, NY.Google Scholar

Copyright information

© Kluwer Academic Publishers 2001

Authors and Affiliations

  • Bernd Simon
    • 1
  • Katia Zanier
    • 1
  • Michael Sattler
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany

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