Applied Biochemistry and Microbiology

, Volume 37, Issue 3, pp 282–286 | Cite as

The Respiratory Activity of Rhodococcus rhodochrousM8 Cells Producing Nitrile-Hydrolyzing Enzymes

  • S. M. Rogacheva
  • O. V. Ignatov


The respiratory activity of Rhodococcus rhodochrousM8 cells containing nitrile hydratase and amidase was studied in the presence of nitriles and amides of carbonic acids. The culturing of cells with acrylonitrile and acrylamide yielding maximum respiratory activity was studied. The optimum conditions for measurements and maintenance of respiratory activity were found. Curves for the linear concentration dependence of cell respiratory activity on 0.01–0.5 mM acrylonitrile, 0.025–1.0 mM acetonitrile, and 0.01–0.1 mM acrylamide were plotted. The selectivity of cell respiratory activity for some substrates was analyzed.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Thompson, L.A., Konwles, C.J., Linton, E.A., and Wyatt, J.M., Chemistry in Britain, 1988, pp. 900-902, 912.Google Scholar
  2. 2.
    Nagasawa, T. and Yamada, H., Trends Biotechnol., 1989, vol. 7, no. 6, pp. 153-158.Google Scholar
  3. 3.
    Nagasawa, T. and Yamada, H., Pure Appl. Chem., 1990, vol. 62, no. 7, pp. 1441-1444.Google Scholar
  4. 4.
    Yamada, H. and Kobayashi, M., Biosci. Biotech. Biochem., 1996, vol. 69, no. 9, pp. 1391-1400.Google Scholar
  5. 5.
    Liu, T.Z., Wang, Y., Kounaves, S.P., and Brush, E.J., Anal. Chem., 1993, vol. 65, no. 21, pp. 3134-3136.Google Scholar
  6. 6.
    Liu, T.Z., Wang, Y., Kounaves, S.P., and Brush, E.J., Anal. Chem., 1995, vol. 67, p. 3134.Google Scholar
  7. 7.
    Ignatov, O.V., Rogacheva, S.M., Khorkina, N.A., Kozulin, S.V., and Ignatov, V.V., Prikl. Biokhim. Mikrobiol., 1998, vol. 34, no. 4, pp. 460-464.Google Scholar
  8. 8.
    Yanenko, A.S., Polyakova, I.N., Astaurova, O.B., and Kozulin, S.V, RF Inventor's Certificate no. 1731814, Byull. Izobret., 1992, no. 17, p. 111.Google Scholar
  9. 9.
    Manual of Methods for General Bacteriology, Gerhardt, F., et al., Eds., Washington: Am. Soc. Microbiol., 1981. Translated under the title Metody obshchei bakteriologiiMoscow: Mir, 1984, vol. 2, pp. 187-188.Google Scholar
  10. 10.
    Astaurova, O.B., Pogorelova, T.E., Fomina, O.R., Polyakova, I.N., and Yanenko, A.S., Biotekhnologiya, 1991, no. 5, pp. 10-14.Google Scholar
  11. 11.
    Sinolitsky, M.K., Rogatcheva, S.M., Poltavskaya, S.V., Sintin, A.A., and Voronin, S.P., Environmental Biotechnology, Principles and Application, 1995, pp. 96-104.Google Scholar
  12. 12.
    Sinolitskii, M.K., Poltavskaya, S.V., Rogacheva, S.M., Sevryugina, I.V., and Voronin, S.P., Prikl. Biokhim. Mikrobiol., 1997, vol. 33, no. 4, pp. 383-387.Google Scholar
  13. 13.
    Akrilonitril (Gigienicheskie kriterii sostoyaniya okruzhayushchei sredy)(Acrylonitrile, Hygienic Criteria of Environmental Condition), Geneva, WHO, 1987, issue 36.Google Scholar
  14. 14.
    Akrilamid (Gigienicheskie kriterii sostoyaniya okruzhayushchei sredy(Acrylamide, Hygienic Criteria of Environmental Condition), Geneva, WHO, 1989, issue 49.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2001

Authors and Affiliations

  • S. M. Rogacheva
    • 1
  • O. V. Ignatov
    • 2
  1. 1.Saratov State UniversitySaratovRussia
  2. 2.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia

Personalised recommendations