Skip to main content
SpringerLink
Log in

Analysis of DSC Data Relating to Proteins Undergoing Irreversible Thermal Denaturation

  • Published:
Journal of Thermal Analysis and Calorimetry Aims and scope Submit manuscript

Abstract

New approaches to the analysis of differential scanning calorimetry (DSC) data relating to proteins undergoing irreversible thermal denaturation have been demonstrated. The experimental approaches include obtaining a set of DSC curves at various scanning rates and protein concentrations, and also reheating experiments. The mathematical methods of analysis include construction of a linear anamorphosis and simultaneous fitting of a theoretical expression for the dependence of the excess heat capacity on temperature to a set of experimental DSC curves. Different kinetic models are discussed: the one-step irreversible model, the model including two consecutive irreversible steps, the Lumry and Eyring model with a fast equilibrating first step, and the whole kinetic Lumry and Eyring model.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P. L. Privalov and S. A. Potekhin, Meth. Enzymol., 131 (1986) 4.

    Article  CAS  Google Scholar 

  2. J. M. Sturtevant, Annu. Rev. Phys. Chem., 38 (1987) 463.

    Article  CAS  Google Scholar 

  3. E. Freire, W. W. Van Osdol, O. L. Mayorga and J. M. Sanchez-Ruiz, Annu. Rev. Biophys. Biophys. Chem., 19 (1990) 159.

    Article  CAS  Google Scholar 

  4. J. M. Sanchez-Ruiz, Subcellular Biochemistry, 24 (1995) 133.

    CAS  Google Scholar 

  5. V. L. Shnyrov, J. M. Sanchez-Ruiz, B. N. Boiko, G. G. Zhadan and E. A. Permyakov, Thermochim. Acta, 302 (1997) 165.

    Article  Google Scholar 

  6. G. I. Makhatadze and P. L. Privalov, Adv. Protein Chem., 47 (1995) 307.

    CAS  Google Scholar 

  7. J. M. Sanchez-Ruiz, J. L. Lopez-Lacomba, M. Cortijo and P. L. Mateo, Biochemistry, 27 (1988) 1648.

    Article  CAS  Google Scholar 

  8. A. E. Lyubarev, B. I. Kurganov, V. N. Orlov and H.-M. Zhou, Biophys. Chem., 79 (1999) 199.

    Article  CAS  Google Scholar 

  9. V. L. Shnyrov, L. D. Martinez, M. G. Roig, A. E. Lyubarev, B. I. Kurganov and E. Villar, Thermochim. Acta, 325 (1999) 143.

    Article  CAS  Google Scholar 

  10. S. A. Potekhin, O. I. Loseva, E. I. Tiktopulo and A. P. Dobritsa, Biochemistry, 38 (1999) 4121.

    Article  CAS  Google Scholar 

  11. B. I. Kurganov, A. E. Lyubarev, J. M. Sanchez-Ruiz and V. L. Shnyrov, Biophys. Chem., 69 (1997) 125.

    Article  CAS  Google Scholar 

  12. M. L. Galisteo, P. L. Mateo and J. M. Sanchez-Ruiz, Biochemistry, 30 (1991) 2061.

    Article  CAS  Google Scholar 

  13. A. Hernandez-Arana, A. Rojo-Dominguez, M. M. Altamirano and M. L. Calcagno, Biochemistry, 32 (1993) 3644.

    Article  CAS  Google Scholar 

  14. D. Grasso, C. La Rosa, D. Milardi and S. Fasone, Thermochim. Acta, 265 (1995) 163.

    Article  CAS  Google Scholar 

  15. B. A. Kornilaev, B. I. Kurganov, T. B. Eronina, N. A. Chebotareva, N. B. Livanova, V. N. Orlov and V. Ya. Chernyak, Mol. Biol. (Moscow), 31 (1997) 98.

    CAS  Google Scholar 

  16. F. Conejero-Lara, A. I. Azuaga and P. L. Mateo, Reactive and Functional Polymers, 34 (1997) 113.

    Article  CAS  Google Scholar 

  17. F. Conejero-Lara, J. M. Sanchez-Ruiz, P. L. Mateo, F. J. Burgos, J. Vendrell and F. X. Aviles, Eur. J. Biochem., 200 (1991) 663.

    Article  CAS  Google Scholar 

  18. A. E. Lyubarev, B. I. Kurganov, A. A. Burlakova and V. N. Orlov, Biophys. Chem., 70 (1998) 247.

    Article  CAS  Google Scholar 

  19. D. I. Kreimer, V. L. Shnyrov, E. Villar, I. Silman and J. Weiner, Protein Sci., 4 (1995) 2349.

    CAS  Google Scholar 

  20. T. Le Bihan and C. Gicquaud, Biochem. Biophys. Res. Commun., 194 (1993) 1065.

    Article  CAS  Google Scholar 

  21. T. Vogl, C. Jatzke, H.-J. Hinz, J. Benz and R. Huber, Biochemistry, 36 (1997) 1657.

    Article  CAS  Google Scholar 

  22. J. Villaverde, J. Cladera, E. Padros, J.-L. Rigaud and M. Dunach, Eur. J. Biochem., 244 (1997) 441.

    Article  CAS  Google Scholar 

  23. J. Villaverde, J. Cladera, A. Hartog, J. Berden, E. Padros and M. Dunach, Biophys. J., 75 (1998) 1980.

    CAS  Google Scholar 

  24. Z.-Y. Wang, E. Freire and R. E. McCarty, J. Biol. Chem., 268 (1993) 20785.

    CAS  Google Scholar 

  25. A. Arroyo-Reyna and A. Hernandes-Arana, Biochim. Biophys. Acta, 1248 (1995) 123.

    Google Scholar 

  26. J. Cervera, F. Conejero-Lara, J. Ruiz-Sanz, M. L. Galisteo, P. L. Mateo, C. J. Lusty and V. Rubio, J. Biol. Chem., 268 (1993) 12504.

    CAS  Google Scholar 

  27. J. M. Sanchez-Ruiz, J. L. Lopez-Lacomba, P. L. Mateo, M. Vilanova, M. A. Serra and F. X. Aviles, Eur. J. Biochem., 176 (1988) 225.

    Article  CAS  Google Scholar 

  28. A. L. Garda-Salas, R. I. Santamaria, M. J. Marcos, G. G. Zhadan, E. Villar and V. L. Shnyrov, Biochem. Mol. Biol. Int., 38 (1996) 161.

    CAS  Google Scholar 

  29. G. G. Zhadan and V. L. Shnyrov, Biochem. J., 299 (1994) 731.

    CAS  Google Scholar 

  30. E. K. Merabet, M. C. Walker, H. K. Yven and J. A. Sikorski, Biochim. Biophys. Acta, 1161 (1993) 272.

    CAS  Google Scholar 

  31. R. F. Epand, R. M. Epand and C. Y. Jung, Biochemistry, 38 (1999) 454.

    Article  CAS  Google Scholar 

  32. N. Singh, Z. Liu and H. F. Fisher, Biophys. Chem., 63 (1996) 27.

    Article  CAS  Google Scholar 

  33. A. Rojo-Domingues, A. Hernandez-Arana, G. Mendoza-Hernandez and J. L. Rendon, Biochem. Mol. Biol. Int., 42 (1997) 631.

    Google Scholar 

  34. M. Guzman-Casado, A. Parody-Morreale, P. L. Mateo and J. M. Sanchez-Ruiz, Eur. J. Biochem., 188 (1990) 181.

    Article  CAS  Google Scholar 

  35. E. A. Saburova, N. N. Khechinashvili and I. I. Elfimova, Mol. Biol., 30 (1996) 1219.

    CAS  Google Scholar 

  36. M. J. Marcos, R. Chehin, J. L. Arrondo, G. G. Zhadan, E. Villar and V. L. Shnyrov, FEBS Lett., 443 (1999) 192.

    Article  CAS  Google Scholar 

  37. J. Davoodi, W. W. Wakarchuk, W. K. Surewicz and P. R. Carey, Protein Sci., 7 (1998) 1538.

    CAS  Google Scholar 

  38. S. A. Potekhin and E. L. Kovrigin, Biophys. Chem., 73 (1998) 241.

    Article  CAS  Google Scholar 

  39. J. M. Sanchez-Ruiz, Biophys. J., 61 (1992) 921.

    Article  CAS  Google Scholar 

  40. D. Milardi, C. La Rosa and D. Grasso, Biophys. Chem., 52 (1994) 183.

    Article  CAS  Google Scholar 

  41. C. La Rosa, D. Milardi, D. Grasso, R. Guzzi and L. Sportelli, J. Phys. Chem., 99 (1995) 14864.

    Article  CAS  Google Scholar 

  42. R. Guzzi, C. La Rosa, D. Grasso, D. Milardi and L. Sportelli, Biophys. Chem., 60 (1996) 29.

    Article  CAS  Google Scholar 

  43. R. Guzzi, L. Sportelli, C. La Rosa, D. Milardi and D. Grasso, J. Phys. Chem. B., 102 (1998) 1021.

    Article  CAS  Google Scholar 

  44. R. Guzzi, L. Sportelli, C. La Rosa, D. Milardi, D. Grasso, M. Ph. Verbeet and G. W. Canters, Biophys. J., 77 (1999) 1052.

    CAS  Google Scholar 

  45. D. Milardi, C. La Rosa, D. Grasso, R. Guzzi, L. Sportelli and C. Fini, Eur. Biophys. J., 27 (1998) 273.

    Article  CAS  Google Scholar 

  46. W. Meijberg, G. K. Schuurman-Wolters, H. Boer, R. M. Scheck and G. T. Robillard, J. Biol. Chem., 273 (1998) 20785.

    Article  CAS  Google Scholar 

  47. A. E. Lyubarev and B. I. Kurganov, Biochemistry (Moscow), 63 (1998) 434.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bakh Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, Moscow, 117071, Russia

    A. E. Lyubarev & B. I. Kurganov

Authors
  1. A. E. Lyubarev
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. I. Kurganov
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lyubarev, A.E., Kurganov, B.I. Analysis of DSC Data Relating to Proteins Undergoing Irreversible Thermal Denaturation. Journal of Thermal Analysis and Calorimetry 62, 51–62 (2000). https://doi.org/10.1023/A:1010102525964

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1010102525964

Search

Navigation