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QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour

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Abstract

Field resistance to Phytophthora infestans, the causal agent of foliage and tuber blight in cultivated potatoes, earliness (maturity) and vigour, were examined in a diploid segregating potato population grown in replicated trials over three consecutive growing seasons. A genetic linkage map of this population was constructed in parallel using PCR-based SSR, AFLP and CAPS markers. Analysis of the trait scores alongside the marker segregation data allowed the identification of regions of the genome which were significantly correlated with components of the respective characters. The most significant associations for all four traits were with marker alleles on potato linkage group V originating from the male (susceptible) parent. In the case of foliage resistance to late blight, the positions of the majority of the effects, which were located on eleven of the twelve potato linkage groups, have been detected in previous [16] and parallel studies [21]. The absence of Solanum demissum-derived R genes for hypersensitive response to late blight and the co-localisation of QTL for resistance, vigour and earliness suggest that developmental and/or physiological factors play a major role in determining the level of foliage resistance in this population. In contrast with previous findings, a negative correlation was found between foliage and tuber blight resistance.

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References

  1. Cruickshank G, Stewart HE, Wastie RL: An illustrated assessment key for foliage blight of potatoes. Potato Res 25: 213–214 (1982).

    Google Scholar 

  2. Elkharbotly A, Leonards-schippers C, Huigen DJ, Jacobsen E, Pereira A, Stiekema WJ, Salamini F, Gebhardt C: Segregation analysis and RFLP mapping of the R1 and R3 alleles conferring race-specific resistance to Phytophthora infestans in progeny of dihaploid potato parents. Mol Gen Genet 242: 749–754 (1994).

    PubMed  Google Scholar 

  3. Elkharbotly A, Palominosanchez C, Salamini F, Jacobsen E, Gebhardt C: R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans [(Mont) de Bary] identified genetic-loci clustering with the R3 locus on Chromosome-XI. Theor Appl Genet 92: 880–884 (1996).

    Article  Google Scholar 

  4. Elkharbotly A, Pereira A, Stiekema WJ, Jacobsen E: Racespecific resistance against Phytophthora infestans in potato is controlled by more genetic-factors than only R-genes. Euphytica 90: 331–336 (1996).

    Article  Google Scholar 

  5. Freyre R, Douches D: Development of a model for markerassisted selection of specific-gravity in diploid potato across environments. Crop Science 34: 1361–1368 (1994).

    Google Scholar 

  6. Fritzemeier KH, Cretin C, Kombrink E, Rohwer F, Taylor J, Scheel D, Hahlbrock K: Transient induction of phenylalanine ammonia-lyase and 4-coumarate-Coa ligase messenger-Rnas in potato leaves infected with virulent or avirulent races of Phytophthora infestans. Plant Physiol 85: 34–41 (1987).

    Google Scholar 

  7. Gebhardt C, Blomendahl C, Schachtschabel U, Debener T, Salamini F, Ritter E: Identification of 2n breeding lines and 4n varieties of potato (Solanum tuberosum ssp tuberosum) with RFLP-fingerprints. Theor Appl Genet 78: 16–22 (1989).

    Article  Google Scholar 

  8. Gebhardt C, Ritter E, Barone A, Debener T, Walkameier B, Schachtschabel U, Kaufmann H, Thompson R, Bonierbale M, Ganal M, Tanksley S, Salamini F: RFLP maps of potato and their alignment with the homeologous tomato genome. Theor Appl Genet 83: 49–57 (1991).

    Article  Google Scholar 

  9. GENSTAT 5.0 Committee: GENSTAT 5.0 Reference Manual. Clarendon Press, Oxford (1987).

    Google Scholar 

  10. Grainger J: Host Nutrition and attack by fungal parasites. Phytopathology 46: 445–446 (1956).

    Google Scholar 

  11. Hanneman RE, Bamburg JB: Inventory of tuber bearing Solanum species. Wisconsin Agricultural Experimental Station Bulletin 533 (1986).

  12. Hodgson WA: Laboratory testing of the of the potato for partial resistance to Phytophthora infestans. Am Potato J 38: 259–264 (1961).

    Google Scholar 

  13. Hoekstra R, Seidewitz L: Evaluation data on tuber-bearing Solanum species, 2nd Edition. German-Dutch curatorium for plant genetic resources. FAL, Braunschweig SVP, Wageningen. (1987).

    Google Scholar 

  14. James RV, Fry WE: Potential for Phytophthora infestans populations to adapt to potato cultivars with rate reducing resistance. Phytopathology 73: 984–988 (1983).

    Google Scholar 

  15. Leonards-Schippers C, Gieffers W, Salamini F, Gebhardt C: The R1 gene conferring race-specific resistance to Phytophthora infestans in potato is located on potato Chromosome-V. Mol Gen Genet 233: 278–283 (1992).

    Article  PubMed  Google Scholar 

  16. Leonards-Schippers C, Gieffers W, Schaferpregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C: Quantitative resistance to Phytophthora infestans in potato: a case-study for QTL mapping in an allogamous plant species. Genetics 137: 67–77 (1994).

    PubMed  Google Scholar 

  17. Li X, van Eck HJ, Rouppe van der Voort JNAM, Huigen DJ, Stam P, Jacobsen E: Autotetraploids and genetic mapping using common AFLP markers: the R2 allele conferring resistance to Phytophthora infestans mapped on potato chromosome 4. Theor Appl Genet 96: 1121–1128 (1998).

    Article  Google Scholar 

  18. Meksem K, Leister D, Peleman J, Zabeau M, Salamini F, Gebhardt C: A high-resolution map of the vicinity of the R1 locus on chromosome-V of potato based on RFLP and AFLP markers. Mol Gen Genet 249: 74–81 (1995).

    Article  PubMed  Google Scholar 

  19. Meyer RC, Milbourne D, Hackett CA, Bradshaw J, McNicol JW, Waugh R: Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestans). Mol Gen Genet 259: 150–160 (1998).

    Article  PubMed  Google Scholar 

  20. Milbourne D, Meyer RC, Collins A, Ramsay L, Gebhardt C, Waugh R: Isolation characterisation and mapping of simple sequence repeat loci in potato. Mol Gen Genet 259: 233–245 (1998).

    Article  PubMed  Google Scholar 

  21. Oberhagemann P, Chatot-Balandras C, Schäfer-Pregl R, Wegener D, Palomino C, Bonnel E, Gebhardt C: Genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection. Mol Breed (in press).

  22. Ordonez ME, Forbes GA, Trognitz BR: Resistance to late blight in potato. A putative gene that suppresses R-genes and is elicited by specific isolates. Euphytica 95: 167–172 (1997).

    Article  Google Scholar 

  23. Ritter E, Debener T, Barone A, Salamini F, Gebhardt C: RFLP mapping on potato chromosomes of two genes controlling extreme resistance to potato virus X (PVX).Mol Gen Genet 227: 81–85 (1991).

    PubMed  Google Scholar 

  24. Rohwer F, Fritzemeier K-H, Scheel D, Hahlbrock K: Biochemical reaction of different tissues of potato (Solanum tuberosum) to zoospores and elicitors from Phytophthora infestans. Planta 170: 556–561 (1987).

    Article  Google Scholar 

  25. Ross H: Potato Breeding: Problems and Perspectives. Advances in Plant Breeding 13, Paul Parey, Berlin/Hamburg (1986).

    Google Scholar 

  26. Simko I, McMurray S, Yang H-M, Manschot A, Davies PJ, Ewing EE: Evidence from polygene mapping for a causal relationship between potato dormancy and abscisic acid content. Plant Physiol 115: 1453–1459 (1997).

    PubMed  Google Scholar 

  27. Stam P: Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP. Plant J. 3: 739–744 (1993).

    Article  Google Scholar 

  28. Stam P, van Ooijen JW: JoinMap version 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen (1995).

    Google Scholar 

  29. Stewart H, Bradshaw J, Wastie R: Correlation between resistance to late blight in foliage and tubers in potato clones from parents of contrasting resistance. Potato Res 39: 429–434 (1994).

    Google Scholar 

  30. Swiezynski KM: Resistance to Phytophthora infestans in the potato. Institute for Potato Research, Bonin (1990).

    Google Scholar 

  31. Tanksley SD: Mapping polygenes. Ann Rev Genet 27: 205–233 (1993).

    Article  PubMed  Google Scholar 

  32. Taylor JT, Fritzemeier I, Hauser E, Kombrink F, Rohwer F, Schroder M, Strittmatter G, Hahlbrock K: Structural analysis and activation by fungal infection of a gene encoding a pathogenesis-related protein in potato. Mol Plant-Microbe Interact 3: 72–77 (1990).

    PubMed  Google Scholar 

  33. Thurston HD: Relationship of general resistance: late blight of potato. Phytopathology 61: 620–626 (1971).

    Google Scholar 

  34. Toxopeus HJ: Some notes on the relations between field resistance to Phytophthora infestans in leaves and tubers and ripening time in Solanum tuberosum ssp. tuberosum. Euphytica 7: 123–130 (1958).

    Article  Google Scholar 

  35. Umaerus V, Umaerus M: Inheritance of resistance to late blight In: Bradshaw JE, Mackay GR (eds) Potato Genetics, pp. 365–401. CAB International, Wallingford, Oxon, UK (1994).

    Google Scholar 

  36. van Eck H: Proceedings of the Thirteenth Triennial Conference of the European Association for Potato Research (1996).

  37. Vanden Berg JH, Ewing EE, Plaisted RL, McMurry S, Bonierbale MW: QTL analysis of potato tuberization. Theor Appl Genet 93: 307–316 (1996).

    Article  Google Scholar 

  38. Vanden Berg JH, Ewing EE, Plaisted RL, McMurry S, Bonierbale MW: QTL analysis of tuber dormancy. Theor Appl Genet 93: 317–324 (1996b).

    Article  Google Scholar 

  39. Vanderplank JE: Stability of resistance to Phytophthora infestans in cultivars without R-genes. Potato Res 14: 263–270 (1971).

    Google Scholar 

  40. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Peleman J, Kuiper M, Zabeau M: AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407–4414 (1995).

    PubMed  Google Scholar 

  41. Wastie R: Breeding for Resistance. In: Ingram DS, Williams PH (eds) Phytophthora infestans, the cause of late blight of potato, Advances in Plant Pathology 7, pp. 193–224. Academic Press, London (1991).

    Google Scholar 

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Authors and Affiliations

  1. Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK

    A. Collins, D. Milbourne, L. Ramsay, R. Meyer, W. De Jong & R. Waugh

  2. Max-Plank-Institut für Züchtungsforschung, Carl-von-Linné-weg 10, 50829, Köln, Germany

    P. Oberhagemann & C. Gebhardt

  3. GERMICOPA, 1, Allée Loeiz Herrieu, 29334, Quimper Cedex, France

    C. Chatot-Balandras & E. Bonnel

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  1. A. Collins
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  2. D. Milbourne
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  3. L. Ramsay
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  4. R. Meyer
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  5. C. Chatot-Balandras
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  6. P. Oberhagemann
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  8. C. Gebhardt
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  9. E. Bonnel
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Collins, A., Milbourne, D., Ramsay, L. et al. QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour. Molecular Breeding 5, 387–398 (1999). https://doi.org/10.1023/A:1009601427062

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