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Effect of some heavy metal ions on copper-induced metallothionein synthesis in the yeast Saccharomyces cerevisiae

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Abstract

Copper-induced metallothionein (MT) synthesis in Saccharomyces cerevisiae was investigated in order to associate this exclusively with Cu2+ in vivo, when cultured in nutrient medium containing other heavy metal ions. Expression of the CUP1 promoter/lacZ fusion gene was inhibited by all heavy metal ions tested, especially Cd2+ and Mn2+. By adding Cd2+ and Mn2+ at 10 μM concentration, the β-galactosidase activity decreased by about 80% and 50% of the maximum induction observed with 1 mM CuSO4, respectively. Furthermore, cell growth was markedly inhibited by combinations of 1 mM-Cu2+ and 1 μM-Cd2+. Therefore, the yeast S. cerevisiae could not rely on MT synthesis as one of the copper-resistance mechanisms, when grown in a Cd2+ environment. In contrast, the presence of Mn2+ in the nutrient medium showed alleviation rather than growth inhibition by high concentrations of Cu2+. The recovery from growth inhibition by Mn2+ was due to decreased Cu2+ accumulation. Inhibitory concentrations of Co2+, Ni2+ and Zn2+ on expression of the CUP1p/lacZ fusion gene were at least one order of magnitude higher than that of Cd2+ and Mn2+. These results are discussed in relation to Cu2+ transport and Cu-induced MT synthesis in the copper-resistance mechanism of the yeast S. cerevisiae.

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Authors and Affiliations

  1. Ehime Prefectural Institute of Public Health and Environmental Science, Sanban-cho, Matsuyama, 790-0003, Japan

    Masa-aki Okuyama

  2. Department of Biology, Faculty of Science, Ehime University, Matsuyama, 790-8577, Japan

    Yoshihiro Kobayashi, Masahiro Inouhe, Hiroshi Tohoyama & Masanori Joho

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  1. Masa-aki Okuyama
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  2. Yoshihiro Kobayashi
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  3. Masahiro Inouhe
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  4. Hiroshi Tohoyama
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  5. Masanori Joho
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Okuyama, Ma., Kobayashi, Y., Inouhe, M. et al. Effect of some heavy metal ions on copper-induced metallothionein synthesis in the yeast Saccharomyces cerevisiae. Biometals 12, 307–314 (1999). https://doi.org/10.1023/A:1009258523040

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