Biotechnology Techniques

, Volume 13, Issue 6, pp 391–393 | Cite as

Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

  • R. Oliveira
  • F. Marques
  • J. Azeredo
Article

Abstract

The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.

differential precipitation polysaccharides proteins 

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References

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • R. Oliveira
    • 1
  • F. Marques
    • 1
  • J. Azeredo
    • 1
  1. 1.Centro de Engenharia Biológica — IBQFUniversidade do MinhoBragaPortugal

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