Abstract
Methylobacterium rhodesianum was cultivated using an extended fed-batch regime. After treatments with N-nitroso-methyl-urea or X-rays, colonies showing an irregular morphology or increased heavy-metal sensitivity were selected. Electron micrographs of cells of isolated strains demonstrated the loss of the glycocalyx. Selectants and the parental strain yielded identical PHB amounts.
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Bormann, E., Roth, M., Linß, W. et al. Selection of capsule deficient strains of Methylobacterium rhodesianum producing polyhydroxybutyrate. Biotechnology Techniques 13, 539–544 (1999). https://doi.org/10.1023/A:1008904814522
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DOI: https://doi.org/10.1023/A:1008904814522