Selection of a strain of Aspergillus for the production of citric acid from pineapple waste in solid-state fermentation

  • C.T. Tran
  • L.I. Sly
  • D.A. Mitchell

Abstract

Aspergillus foetidus ACM 3996 (=FRR 3558) and three strains of Aspergillus niger ACM 4992 (=ATCC 9142), ACM 4993 (=ATCC 10577), ACM 4994 (=ATCC 12846) were compared for the production of citric acid from pineapple peel in solid-state fermentation. A. niger ACM 4992 produced the highest amount of citric acid, with a yield of 19.4g of citric acid per 100g of dry fermented pineapple waste under optimum conditions, representing a yield of 0.74g citric acid/g sugar consumed. Optimal conditions were 65% (w/w) initial moisture content, 3% (v/w) methanol, 30°C, an unadjusted initial pH of 3.4, a particle size of 2mm and 5ppm Fe2+. Citric acid production was best in flasks, with lower yields being obtained in tray and rotating drum bioreactors.

Aspergillus niger citric acid pineapple waste solid-state fermentation 

References

  1. Bodie, E.A., Bower, B., Berka, R.M. & Dunn-Coleman, N.S. 1994 Economically important organic acid and enzyme products. In: Aspergillus 50 Years of Progress in Industrial Microbiology, eds Martinella, S.D. & Kinghoron, J.R., pp. 562–568. Amsterdam: Elsevier.Google Scholar
  2. Clark, D.S., Ito, K. & Horistsu, H. 1966 Effect of manganese and other heavy metals on submerged citric acid fermentation of molasses. Biotechnology and Bioengineering 8, 465–469.Google Scholar
  3. Hang, Y.D. 1988 Microbial production of citric acid in fixed bed column bioreactors. Biotechnology Letters 10, 421–426.Google Scholar
  4. Hang, Y.D. & Woodams, E.E. 1984 Apple pomace: A potential substrate for citric acid production by Aspergillus niger. Biotechnology Letters 6, 763–764.Google Scholar
  5. Hang, Y.D. & Woodams, E.E. 1985 Grape pomace: A novel substrate for microbial production of citric acid. Biotechnology Letters 7, 253–254.Google Scholar
  6. Hang, Y.D. & Woodams, E.E. 1986a Utilization of grape pomace for citric acid production by solid state fermentation. American Journal of Enology and Viticulture 37, 141–142.Google Scholar
  7. Hang, Y.D. & Woodams, E.E. 1986b Solid state fermentation of apple pomace for citric acid production. MIRCEN Journal of Applied Microbiology and Biotechnology 2, 283–287.Google Scholar
  8. Hang, Y.D. & Woodams, E.E. 1987 Effect of substrate moisture content on fungal production of citric acid in a solid state fermentation system. Biotechnology Letters 9, 183–186.Google Scholar
  9. Hang, Y.D., Luh, B.S. & Woodams, E.E. 1987 Microbial production of citric acid by solid state fermentation of kiwifruit peel. Journal of Food Science 52, 226–227.Google Scholar
  10. Kapoor, K.K., Chaudhary, K. & Tauro, P. 1982 Citric acid. In Prescott and Dunn's Industrial Microbiology, 4th edn, ed. Reed, G., pp. 709–747. Westport: The AVI Publishing Company.Google Scholar
  11. Lakshminarayana, K., Chaudhary, K. Ethiraj, S. & Tauro, P. 1975 A solid state fermentation method for citric acid production using sugar cane bagasse. Biotechnology and Bioengineering 17, 291–293.Google Scholar
  12. Lonsane, B.K., Ghildyal, N.P., Budiatman, S. & Ramakrishna, S.V. 1985 Engineering aspects of solid state fermentation. Enzyme and Microbial Technology 7, 258–265.Google Scholar
  13. Lu, M.Y., Brook, J.D. & Maddox, I.S. 1995 Citric acid production by A. niger in solid state fermentation. Proceedings of the 4th Pacific Rim Biotechnology Conference, Melbourne, 6–9 February, pp. 219–220.Google Scholar
  14. Mitchell, D.A., Targonski, Z., Rogalski, J. & Leonowicz, A. 1992 Substrate for processes. In Solid Substrate Cultivation, eds Doelle, H.W., Mitchell, D.A. & Rolz, C.E., pp. 29–51. Barking: Elsevier Science Publishers.Google Scholar
  15. Noyes, R. 1969 Citric acid Production Processes. Parkridge: Noyes Development Corporation.Google Scholar
  16. Purohit, H.J. & Daginawala, H.F. 1986 The relationship of some metal ions with citric acid production by Aspergillus niger using tamarind seed powder as raw material. Journal of Fermentation Technology 64, 561–565.Google Scholar
  17. Rohr, M., Kubicek, C.P. & Komineck, J. 1983 Citric acid. In Biotechnology, Vol. 3, Biomass, Microorganisms for Special Applications, Microbial Products I, Energy from Renewable Sources, ed. Dellweg, H., pp. 420–454. Weinheim: VCH Verlag Chemie.Google Scholar
  18. Roukas, T. & Kotzekidou, P. 1987 Influence of some trace metals and stimulants on citric acid production from brewery wastes by Aspergillus niger. Enzyme and Microbial Technology 9, 291–293.Google Scholar
  19. Shankaranand, V.S. & Lonsane, B.K. 1992 Wheat bran as a substrate for production of citric acid in solid state fermentation system: Titre and yield improvements. Chemie Microbiologie Technologie der Lebersmittel 14, 33–39.Google Scholar
  20. Shankaranand, V.S. & Lonsane, B.K. 1993 Sugar cane-pressmud as a novel substrate for production of citric acid by solid state fermentation. World Journal of Microbiology and Biotechnology 9, 377–380.Google Scholar
  21. Shankaranand, V.S. & Lonsane, B.K. 1994a Coffee husk: an inexpensive substrate for production of citric acid by Aspergillus niger in a solid state fermentation system. World Journal of Microbiology & Biotechnology 10, 165–168.Google Scholar
  22. Shankaranand, V.S. & Lonsane, B.K. 1994b Ability of Aspergillus niger to tolerate metal ions and minerals in a solid state fermentation system for the production of citric acid. Process Biochemistry 29, 29–37.Google Scholar
  23. Shu, P. & Johnson, M.J. 1948 The independence of medium constituents in citric acid production by submerged fermentation. Journal of Bacteriology 56, 577–585.Google Scholar
  24. Tomilsom, N., Campbell, J.J.R. & Trussel, P.C. 1951 The influence of zinc, iron, copper and manganese on the production of citric acid by Aspergillus niger. J. Bacteriol. 61, 17–25.PubMedGoogle Scholar
  25. Tran, C.T. & Mitchell, D.A. 1995 Pineapple waste–a novel substrate for citric acid production by solid-state fermentation. Biotechnology Letters 17, 1107–1110.Google Scholar

Copyright information

© Rapid Science Publishers 1998

Authors and Affiliations

  • C.T. Tran
    • 1
    • 2
  • L.I. Sly
    • 3
  • D.A. Mitchell
    • 1
  1. 1.Department of Chemical EngineeringThe University of QueenslandBrisbaneAustralia; fax
  2. 2.Dong Da, HanoiVietnam; fax
  3. 3.Department of MicrobiologyThe University of QueenslandBrisbaneAustralia; fax

Personalised recommendations