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Journal of Biomolecular NMR

, Volume 13, Issue 4, pp 381–385 | Cite as

A novel NMR experiment for the sequential assignment of proline residues and proline stretches in 13C/15N-labeled proteins

  • M.J. Bottomley
  • M.J. Macias
  • Z. Liu
  • M. Sattler
Article

Abstract

A new pulse sequence is described for the sequential assignment of proline residues in 13C/15N-labeled proteins by correlating Cδ and Cα chemical shifts of proline residues with the Hα chemical shift of the preceding residue. Notably, the experiment can provide the sequential connectivities in poly-proline stretches, which cannot be determined using standard triple resonance experiments. Excellent solvent suppression is achieved by coherence selection via a heteronuclear gradient echo. The new pulse sequence has been successfully applied to the 11 kDa HRDC domain.

proline proline-rich selective pulses sequential assignment triple resonance 

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • M.J. Bottomley
    • 1
  • M.J. Macias
    • 1
  • Z. Liu
    • 1
  • M. Sattler
    • 1
  1. 1.European Molecular Biology LaboratoryHeidelbergGermany

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