Advertisement

Polysaccharides from the red seaweed Bostrychia montagnei: chemical characterization

  • Miguel D. Noseda
  • Siumara Tulio
  • Maria E. R. Duarte
Article

Abstract

Bostrychia montagnei was submitted to aqueous extraction at 25 and 85 °C. The purified polysaccharide extracts represent ∼ 17% of the dried alga. Galactose is the principal monosaccharide component of these extracts (60.8–70.4 mol%). 3,6-Anhydrogalactose and its 2- O-methyl derivative are also present in smaller amounts (16.2–22.0 mol%), as well as other methylated sugars, such as 6- O- (6.5–7.8 mol%) and 2-O-methylgalactose (0.2–2.1 mol%). Xylose (4.1–8.1 mol%) and glucose (0.7–2.6 mol%) were also detected. The aqueous extracted polysaccharides (25 °C) were separated by anion-exchange chromatography into six sulphated galactan fractions with negative specific rotations and another two with high xylose contents and positive specific rotations. The sulphated galactans all have an agar type backbone modified by partial O-methyl substitution on O-6 or O-2 of the galactosyl units. The latter substitution is also present in varying degrees of 3,6-anhydrogalactose.

seaweed Bostrychia montagnei polysaccharides sulphated galactans 

References

  1. Batey JF, Turvey JR (1975) The galactan sulphate of the red alga Polysiphonia lanosa. Carbohydr. Res. 43: 133–143.PubMedCrossRefGoogle Scholar
  2. Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal. Biochem. 54: 484–489.PubMedCrossRefGoogle Scholar
  3. Bowker DM, Turvey JR (1968) Water-soluble polysaccharides from the red alga Laurencia pinnatifida. Part I. Constituent units. J. chem. Soc., C. 983–988.Google Scholar
  4. Cases MR, Stortz CA, Cerezo AS (1992) Methylated, sulphated xylogalactans from the red seaweed Corallina officinalis. Phytochemistry 31: 3897–3900.CrossRefGoogle Scholar
  5. Dodgson KS, Price RG (1962) A note on the determination of the ester sulphate content of sulphated polysaccharides. Biochem. J. 84: 106–110.PubMedGoogle Scholar
  6. Dubois MK, Gilles A, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.CrossRefGoogle Scholar
  7. Duckworth M, Yaphe W (1971) The structure of agar. Part I. Fractionation of a complex mixture of polysaccharides. Carbohydr. Res. 16: 189–197.CrossRefGoogle Scholar
  8. Furneaux RH, Stevenson TT (1990) The xylogalactan sulfate from Chondria macrocarpa (Ceramiales, Rhodophyta). Hydrobiologia 204/205: 615–620.CrossRefGoogle Scholar
  9. Karsten U, West JA, Zuccarello G (1992) Polyol content of Bostrychia and Stictosiphonia (Rhodomelaceae, Rhodophyta) from field and culture. Bot. mar. 35: 11–19.Google Scholar
  10. Kolender AA, Matulewicz MC, Cerezo AS (1995) Structural analysis of antiviral sulfated α-D-(1→3)-linked mannans. Carbohydr. Res. 273: 179–185.PubMedCrossRefGoogle Scholar
  11. Kremer BP (1976) Distribution of alditols in the genus Bostrychia. Biochem. System. Ecol. 4: 139–141.CrossRefGoogle Scholar
  12. Lowry OH, Rosebrough NJ, Farr AL, Randall RL (1951) Protein measurement with Folin phenol reagent. J. biol. Chem. 193: 265–275.PubMedGoogle Scholar
  13. Matulewicz MC, Cerezo AS, Jarret RM, Syn N (1992) High resolution 13C-n.m.r. spectroscopy of ‘mixed linkage’ xylans. Int. J. biol. Macromol. 14: 29–32.PubMedGoogle Scholar
  14. Matulewicz MC, Ciancia M, Noseda MD, Cerezo AS (1989) Carrageenan systems from tetrasporic and cystocarpic stages of Gigartina skottsbergii. Phytochemistry 28: 2932–2941.CrossRefGoogle Scholar
  15. Miller IJ, Furneaux RH (1997) The structural determination of the agaroid polysaccharides from four New Zealand algae in the order Ceramiales by means of 13C NMR spectroscopy. Bot. mar. 40: 333–339.CrossRefGoogle Scholar
  16. Miller IJ, Falshaw R, Furneaux RH (1993) The chemical structure of polysaccharides from New Zealand members of the Rhodomelaceae. Bot. mar. 36: 203–208.Google Scholar
  17. Painter TJ (1983) In Aspinall GO (ed.), The Polysaccharides Vol. 2, Academic Press, New York: 195–285.Google Scholar
  18. Park JT, Johnson MJ (1949) A submicro determination of glucose. J. biol. Chem. 181: 149–151.PubMedGoogle Scholar
  19. Rees DA (1961) Estimation of the relative amounts of isomeric sulphate esters in some sulphated polysaccharides. J. chem. Soc. 5168–5171.Google Scholar
  20. Stevenson TT, Furneaux RH (1991) Chemical methods for the analysis of sulphated galactans from red algae. Carbohydr. Res. 210: 277–298.PubMedCrossRefGoogle Scholar
  21. Stortz CA, Cerezo AS (1993) The systems of carrageenans from cystocarpic and tetrasporic stages from Iridaea undulosa: fractionation with potassium chloride and methylation analysis of the fractions. Carbohydr. Res. 242: 217–227.CrossRefGoogle Scholar
  22. Usov AI (1992) Sulfated polysaccharides of the red seaweeds. Food Hydrocolloids 6: 9–23.CrossRefGoogle Scholar
  23. Usov AI, Elashvili MYa (1991) Polysaccharides of algae. 44. Investigation of sulfated galactan from Laurencia nipponica Yamada (Rhodophyta, Rhodomelaceae) using partial reductive hydrolysis. Bot. mar. 34: 553–560.CrossRefGoogle Scholar
  24. Yaphe W (1960) Colorimetric determination of 3,6-anhydrogalactose and galactose in marine algal polysaccharides. Anal. Chem. 32: 1327–1330.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • Miguel D. Noseda
    • 1
  • Siumara Tulio
    • 1
  • Maria E. R. Duarte
    • 1
  1. 1.Departamento de Bioquímica-UFPRCentro PolitécnicoCuritiba, ParanáBrasil

Personalised recommendations