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Cytotechnology

, Volume 24, Issue 1, pp 55–64 | Cite as

Chinese hamster ovary cells produce sufficient recombinant insulin-like growth factor I to support growth in serum-free medium. Serum-free growth of IGF-I-producing CHO cells

  • S. M. N. Hunt
  • S. C. O. Pak
  • M. W. Bridges
  • P. P. Gray
  • M. J. Sleigh
Article

Abstract

Insulin-like growth factor I has similar mitogenic effects to insulin, a growth factor required by most cells in culture, and it can replace insulin in serum-free formulations for some cells. Chinese Hamster Ovary cells grow well in serum-free medium with insulin and transferrin as the only exogenous growth factors. An alternative approach to addition of exogenous growth factors to serum-free medium is transfection of host cells with growth factor-encoding genes, permitting autocrine growth. Taking this approach, we constructed an IGF-I heterologous gene driven by the cytomegalovirus promoter, introduced it into Chinese Hamster Ovary cells and examined the growth characteristics of Insulin-like growth factor I-expressing clonal cells in the absence of the exogenous factor. The transfected cells secreted up to 500 ng/106 cells/day of mature Insulin-like growth factor I into the conditioned medium and as a result they grew autonomously in serum-free medium containing transferrin as the only added growth factor. This growth-stimulating effect, observed under both small and large scale culture conditions, was maximal since no further improvement was observed in the presence of exogenous insulin.

CHO IGF-I serum-free autocrine growth cell culture 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • S. M. N. Hunt
    • 1
  • S. C. O. Pak
    • 1
  • M. W. Bridges
    • 1
  • P. P. Gray
    • 1
  • M. J. Sleigh
    • 2
  1. 1.Department of BiotechnologyUniversity of New South WalesKensingtonAustralia
  2. 2.Division of Biomolecular EngineeringCSIRONorth RydeAustralia

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