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Cytotechnology

, Volume 24, Issue 1, pp 11–17 | Cite as

A new astrocytic cell line which is able to induce a blood-brain barrier property in cultured brain capillary endothelial cells

  • Carsten T. Beuckmann
  • Katja Dernbach
  • Ansgar Hakvoort
  • Hans-Joachim Galla
Article

Abstract

Astrocytes, a member of the glial cell family in the central nervous system, are assumed to play a crucial role in the formation of the blood-brain barrier (BBB) in vertebrates. It was shown that astrocytes induce BBB-properties in brain capillary endothelial cells (BCEC) in vitro. We now established an astroglial cell line of non-tumoral origin. The cloned cell line (A7) shows a highly increased proliferation rate and expresses the astrocytic marker glial fibrillary acidic protein. Furthermore, the clone A7 expresses S-100-protein and vimentin, which are also expressed by primary cultured astrocytes. This cell line therefore shows general astrocytic features. In addition, we were able to show that A7 cells re-induce the BBB-related marker enzyme alkaline phosphatase in BCEC, when these two cell types are co-cultured. Thus we have a cell line which can be readily cultured in large quantities, shows common astrocyte properties and is able to influence BCEC with respect to a BBB-related feature.

astrocytes blood-brain barrier clone continuous cell line 

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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Carsten T. Beuckmann
    • 1
  • Katja Dernbach
    • 1
  • Ansgar Hakvoort
    • 1
  • Hans-Joachim Galla
    • 1
  1. 1.Institut für BiochemieWestfälische Wilhelms-UniversitätMünster, F.RGermany

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