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In vitro steroidogenesis by gonads of the Russian sturgeon, Acipenser gueldenstaedti Brandt

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Abstract

Ovaries and testes from the Russian sturgeon at different stages of sexual maturity were incubated with ;sup3;H-androstenedione or 3H-pregnenolone. The major metabolites of androstenedione in both sexes were testosterone and 5α- and 5ß-androstanediols, but no evidence was found for the gonadal production of 11-oxygenated androgens. Pregnenolone was converted to 17-hydroxyprogesterone, androstenedione and testosterone together with reduced metabolites. 11-Oxygenated androgens were found in female serum by gas chromatography-mass spectrometry (GC-MS), in serum of both sexes by radioimmunoassay (RIA), and was detectable by RIA in interrenal but not gonadal tissue. The results suggest that sturgeon may differ from teleosts in that 11-oxygenation may take place in extragonadal tissues.

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Authors and Affiliations

  1. Physiological Institute, St. Petersburg University, University emb., 7/9, St. Petersburg, 199034, Russia

    O. Bukovskaya

  2. Department of Experimental Zoology, Research Group Comparative Endocrinology, University of Utrecht, Padualaan 8, 3584, Utrecht, The Netherlands

    J.G.D. Lambert

  3. Department of Animal and Plant Sciences, The University of Sheffield, Sheffield, S10 2TN, UK

    D.E. Kime

Authors
  1. O. Bukovskaya
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  2. J.G.D. Lambert
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  3. D.E. Kime
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Bukovskaya, O., Lambert, J. & Kime, D. In vitro steroidogenesis by gonads of the Russian sturgeon, Acipenser gueldenstaedti Brandt . Fish Physiology and Biochemistry 16, 345–353 (1997). https://doi.org/10.1023/A:1007784020290

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