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Kinetics of Na+-, K+-ATPase Inhibition by an Endogenous Modulator (II-A)

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Abstract

We have previously reported the isolation by gel filtration and anionic exchange HPLC of two brain Na+, K+-ATPase inhibitors, II-A and II-E, and kinetics of enzyme interaction with the latter. In the present study we evaluated the kinetics of synaptosomal membrane Na+, K+-ATPase with II-A and found that inhibitory activity was independent of ATP (2–8 mM), Na+ (3.1–100 mM), or K+ (2.5–40 mM) concentration. Hanes-Woolf plots showed that II-A decreases Vmax in all cases; KM value decreased for ATP but remained unaltered for Na+ and K+, indicating respectively uncompetitive and noncompetitive interaction. However, II-A became a stimulator at 0.3 mM K+ concentration. It is postulated that brain endogenous factor II-A may behave as a sodium pump modulator at the synaptic region, an action which depends on K+ concentration.

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Authors and Affiliations

  1. Instituto de Biología Celular y Neurociencias “Prof. Eduardo De Robertis”, PROBICENE-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Buenos Aires, Argentina

    A. Reinés

  2. IQUIFIB, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina

    C. Peña

  3. Instituto de Biología Celular y Neurociencias “Prof. Eduardo De Robertis”, PROBICENE-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, 1121, Buenos Aires, Argentina

    G. Rodríguez de Lores Arnaiz

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Reinés, A., Peña, C. & Rodríguez de Lores Arnaiz, G. Kinetics of Na+-, K+-ATPase Inhibition by an Endogenous Modulator (II-A). Neurochem Res 25, 121–127 (2000). https://doi.org/10.1023/A:1007599718356

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