Abstract
Self-incompatibility (SI) in Brassicaceae is genetically controlled by the S locus complex in which S locus glycoprotein (SLG) and S receptor kinase (SRK) genes have been identified, and these two genes encoding stigma proteins are believed to play important roles in SI recognition reaction. Here we introduced the SLG43 gene of Brassica rapa into a self-incompatible cultivar, Osome, of B. rapa, and examined the effect of this transgene on the SI behavior of the transgenic plants. Preliminary pollination experiments demonstrated that Osome carried S52 and S60, and both were codominant in stigma, but S52 was dominant to S60 in pollen. S43 was found to be recessive to S52 and codominant with S60 in stigma. The nucleotide sequence of SLG43 was more similar to that of SLG52 (87.8% identity) than to that of SLG60 (74.8% identity). Three of the ten primary transformants (designated No. 1 to No. 10) were either completely (No. 9) or partially (No. 6 and No. 7) self-compatible; the SI phenotype of the stigma was changed from S52S60 to S60, but the SI phenotype of the pollen was not altered. In these three plants, the mRNA and protein levels of both SLG43 and SLG52 were reduced, whereas those of SLG60 were not. All the plants in the selfed progeny of No. 9 and No. 6 regained SI and they produced a normal level of SLG52. These results suggest that the alteration of the SI phenotype of the stigma in the transformants Nos. 6, 7, and 9 was the result of specific co-suppression between the SLG43 transgene and the endogenous SLG52 gene. Three of the transformants (Nos. 5, 8 and 10) produced SLG43 protein, but their SI phenotype was not altered. The S60 homozygotes in the selfed progeny of No. 10 which produced the highest level of SLG43 were studied because S43 was codominant with S60 in the stigma. They produced SLG43 at approximately the same level as did S43S60 heterozygotes, but did not show S43 haplotype specificity at the stigma side. We conclude that SLG is necessary for the expression of the S haplotype specificity in the stigma but the introduction of SLG alone is not sufficient for conferring a novel S haplotype specificity to the stigma.
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References
An, E.G., Mitra, A. and Ha, S. 1988. Binary vectors. In: S.B. Gelvin, R.A. Schilperoort and D.P.S. Verma (Eds.), Plant Molecular Biology Manual. Kluwer Academic Publishers, Dordrecht, Netherlands, pp. A3/1-A3/19.
Bateman, A.J. 1955. Self-incompatibility systems in angiosperms. III. Cruciferae. Heredity 9: 52-68.
Boyes, D.C., Chen, C.H., Tantikanjana, T., Esch, J.J. and Nasrallah, J.B. 1991. Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci. Genetics 127: 221-228.
Chen, C.H. and Nasrallah, J.B. 1990. A new class of S sequences de-fined by a pollen recessive self-incompatibility allele of Brassica oleracea. Mol. Gen. Genet. 222: 241-248.
Conner, J.A., Tantikanjana, T., Stein, J.C., Kandasamy, M.K., Nasrallah, J.B. and Nasrallah, M.E. 1997. Transgene-induced silencing of S-locus genes and related genes in Brassica. Plant J. 11: 809-823.
Delorme, V., Giranton, J.-L., Hatzfeld, Y., Friry, A., Heizmann, P., Ariza, J., Dumas, C., Gaude, T. and Cock, J.M. 1995. Characterization of the S locus genes, SLG and SRK, of the Brassica S3 haplotype: identification of a membrane-localized protein encoded by the S locus receptor kinase gene. Plant J. 7: 429-440.
Dwyer, K.G., Balent, M.A., Nasrallah, J.B. and Nasrallah, M.E. 1991. DNA sequences of self-incompatibility genes from Brassica campestris and Brassica oleracea: polymorphism predating speciation. Plant Mol. Biol. 16: 481-486.
Goring, D.R. and Rothstein, S.J. 1992. The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase. Plant Cell 4: 1273-1281.
Hatakeyama, K., Takasaki, T., Watanabe, M. and Hinata, K. 1998a. Molecular characterization of S locus genes, SLG and SRK, in a pollen-recessive self-incompatibility haplotype of Brassica rapa L. Genetics 149: 1587-1597.
Hatakeyama, K., Watanabe, M., Takasaki, T., Ojima, K. and Hinata, K. 1998b. Dominance relationships between S-alleles in selfincompatible Brassica campestris L. Heredity 241: 241-247.
Hinata, K., Watanabe, M., Toriyama, K. and Isogai, A. 1993. A review of recent studies on homomorphic self-compatibility. Int. Rev. Cytol. 143: 257-296.
Hood EE, Helmer GL, Fraley RT, Chilton MD: The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J. Bact. 168: 1291-1301.
Isogai, A., Takayama, S., Tsukamoto, C., Ueda, Y., Shiozawa, H., Hinata, K., Okazaki, K. and Suzuki, A. 1987. S-locus-specific glycoproteins associated with self-incompatibility in Brassica campestris. Plant Cell Physiol. 28: 1279-1291.
Kho, Y.O. and Bear, J. 1968. Observing pollen tubes by means of fluorescence. Euphytica 17: 298-302.
Murray, M.G. and Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucl. Acids Res. 8: 4321-4325.
Nasrallah, J.B. and Nasrallah, M.E. 1993. Pollen-stigma signaling in the sporophytic self-incompatibility response. Plant Cell 5: 1325-1335.
Nasrallah, J.B., Kao, T.H., Chen, C.H., Goldberg, M.L. and Nasrallah, M.E. 1987. Amino-acid sequence of glycoproteins encoded by three alleles of the S-locus of Brassica oleracea. Nature 326: 617-619.
Nasrallah, M.E., Kandasamy, M.K. and Nasrallah, J.B. 1992. A genetically defined trans-acting locus regulates S-locus function of Brassica. Plant J. 2: 497-506.
Nishio, T., Toriyama, K., Sato, T., Kandasamy, M.K., Paolillo, D.J., Nasrallah, J.B. and Nasrallah, M.E. 1992. Expression of S-locus glycoprotein genes from Brassica oleracea and B. campestris in transgenic plants of self-compatible B. napus cv. Westar. Sex. Plant Reprod. 5: 101-109.
Nishio, T., Kusaba, M., Watanabe, M. and Hinata, K. 1996. Registration of S alleles in Brassica campestris L. by the restriction fragment sizes of SLGs. Theor. Appl. Genet. 92: 388-394.
Onouchi, H., Tokoi, K., Machida, C., Matsuzaki, H., Oshima, Y., Matsuoka, K., Nakamura, K. and Machida, Y. 1991. Operation of an efficient site-specific recombination system on Zygosaccharomyces rouxii in tobacco cells. Nucl. Acids Res. 19: 6373-6378.
Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
Shiba, H., Hinata, K., Suzuki, A. and Isogai, A. 1995. Breakdown of self-incompatibility in Brassica by the antisense RNA of the SLG gene. Proc. Jpn. Acad. 71: 81-83.
Stahl, R.J., Arnoldo, M., Glavin, T.L., Goring, D.R. and Rothstein, S.J. 1998. The self-incompatibility phenotype is altered by the transformation of the mutant S locus receptor kinase. Plant Cell 10: 209-218.
Stein, J.C., Howlett, B., Boyes, D.C., Nasrallah, M.E. and Nasrallah, J.B. 1991. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc. Natl. Acad. Sci. USA 88: 8816-8820.
Takasaki, T., Hatakeyama, K., Ojima, K., Watanabe, M., Toriyama, K. and Hinata, K. 1997. Factors influencing Agrobacteriummediated transformation of Brassica rapa. L. Breed. Sci. 47: 127-134.
Toriyama, K., Stein, J.C., Nasrallah, M.E. and Nasrallah, J.B. 1991. Transformation of Brassica oleracea with an S-locus gene from B. campestris changes the self-incompatibility phenotype. Theor. Appl. Genet. 81: 769-776.
Yamakawa, S., Shiba, H., Watanabe, M., Shiozawa, H., Takayama, S., Hinata, K., Isogai, A. and Suzuki, A. 1994. The sequence of S glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae. Biosci. Biotechnol. Biochem. 58: 921-925.
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Takasaki, T., Hatakeyama, K., Watanabe, M. et al. Introduction of SLG (S locus glycoprotein) alters the phenotype of endogenous S haplotype, but confers no new S haplotype specificity in Brassica rapa L.. Plant Mol Biol 40, 659–668 (1999). https://doi.org/10.1023/A:1006274525421
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DOI: https://doi.org/10.1023/A:1006274525421