Promotion of indole alkaloid production in Catharanthus roseus cell cultures by rare earth elements

Abstract

Production of the indole alkaloids, ajmalicine or catharanthine, in cell suspension cultures of Catharanthus roseus was enhanced by cerium (CeO₂ and CeCl₃), yttrium (Y₂O₃) and neodymium (NdCl₃). The yield of ajmalicine in these treated-cultures reached 51 mg l⁻¹ (CeO₂), 40 mg l⁻¹ (CeCl₃), 41 mg l⁻¹ (Y₂O₃) and 49 mg l⁻¹ (NdCl₃) while catharanthine production reached to 36 mg l⁻¹ (CeO₂) and 31 mg l⁻¹ (CeCl₃). A major portion of increased alkaloids was released into medium in these treatments. But Sm₂O₃, SmCl₃, La₂O₃, LaCl₃, complex of chromium (III)-titanium (IV) and NaSeO₄ treatments had little effect on alkaloid production of C. roseus cell cultures.