Biotechnology Letters

, Volume 21, Issue 4, pp 265–269 | Cite as

Singlet oxygen quenching ability of astaxanthin esters from the green alga Haematococcus pluvialis

  • M. Kobayashi
  • Y. Sakamoto


The singlet oxygen quenching activities of carotenoids, β-carotene, free astaxanthin, its monoester and its diester, were examined in vitro by a simple and rapid method for the measurement of Methylene Blue-sensitized photooxidation of linoleic acid in the hexane/ethanol solvent system. The concentrations of carotenoids, β-carotene, free astaxanthin, its mono- and di-ester, required for 50% inhibition of lipid oxidation were 40, 8, 9, and 0 μM in 100% ethanol and, 14, 16, 10, and 7 μM in 50% (v/v) hexane in ethanol, respectively. Astaxanthin esters function as powerful antioxidant agents under both hydrophobic and hydrophilic conditions.

astaxanthin carotenes Haematococcus pluvialis oxidation singlet oxygen 


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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • M. Kobayashi
    • 1
  • Y. Sakamoto
    • 1
  1. 1.Research Laboratory of Higashimaru Shoyu Co., Ltd.Tatsuno, HyogoJapan

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