Skip to main content
SpringerLink
Log in

Genetic relationships among grapevine varieties grown in different French and Spanish regions based on RAPD markers

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The genetic relationships among 32 white grapevine varieties (Vitis vinifera L.) grown in different French and Spanish regions were surveyed by using RAPD markers. Two hundred and eight clear and unambiguous RAPD markers were amplified by using 33 primers previously selected. Genetic relationships were established by UPGMA cluster analysis and also by studying RAPD low frequency bands. Three groups of varieties were clearly defined. The stability of the genetic structuration was surveyed by Student t-test and the confidence for certain nodes of the dendrogram was confirmed by bootstrap method. Varieties were separated in clear geographical groups with Atlantic or Mediterranean influence. Some of them were classified according to a common cultivation area and ampelographic characters, suggesting a common origin. In addition, ‘Albariño’, ‘Caíño Blanco’ and ‘Loureiro Blanco’ from Galicia (Spain) and ‘Melon’, ‘Chardonnay’ and ‘Aligoté’ from Burgundy (France) were closely related varieties. Two new closely related couples were defined as ‘Godello’ and ‘Verdejo’, and ‘Malvar’ and ‘Malvasía’.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altube, H., F. Cabello & J. Ortíz, 1991. Caracterización de variedades y portainjertos de vid mediante isoenzimas de los sarmientos. Vitis 30: 203–212.

    CAS  Google Scholar 

  • Arulsekar, S. & D.E. Parfitt, 1986. Isozyme analysis procedures for stone fruits, almond, grape, walnut, pistachio and fig. Hortscience 21(4): 928–933.

    CAS  Google Scholar 

  • Benin, M., J. Gasquez, A. Mahfoudi & R. Bessis, 1988. Caractérisation biochimique des cépages de Vitis vinifera L. par électrophorèse d'isoenzymes foliaires: Essai de classification des variétés. Vitis 27: 157–172.

    CAS  Google Scholar 

  • Bisson, J., 1995. Les principaux groupes écogéographiques dans l'eucépagement Français. Journal Int. des Sciences de la Vigne et du Vin 29(2): 63–68.

    Google Scholar 

  • Borrego, J. & J.F. Gallego, 1990. Descripciones ampelograficas nacionales. 250 pp. Imp. Comunidad de Madrid. Madrid, Spain.

    Google Scholar 

  • Bourquin, J.C., A. Sonko, L. Otten & B. Walter, 1993. Restriction Fragment Length Polymorphism and Molecular Taxonomy in Vitis vinifera L. Theor Appl Genet 87: 431–438.

    Article  CAS  Google Scholar 

  • Bowers, J.E., G.S. Dangi, R. Vignani & C.P. Meredith, 1996. Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinífera L.). Genome 39: 628–633.

    CAS  Google Scholar 

  • Bowers, J.E. & C.P. Meredith, 1997. The parentage of a classic wine grape, Cabernet Sauvignon. Nature genetics 16: 84–87.

    Article  PubMed  CAS  Google Scholar 

  • Büscher, N., E. Zyprian, O. Bachmann & R. Blaich, 1994. On the origin of the grapevine variety Müller-Thurgau as investigated by the inheritance of random amplified polymorphic DNA (RAPD). Vitis 33: 15–17.

    Google Scholar 

  • Clemente, S. de R., 1807. Ensayo sobre las variedades de vid común que vegetan en Andalucia, Madrid.

  • Comenge, M., 1942. La vid y los vinos Españoles. Talleres gráficos Marsiega. Madrid.

  • Felsenstein, J., 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4): 783–791.

    Article  Google Scholar 

  • Freijanes, J. & M.P. Alonso, 1997. Videiras Galegas. Catálogo de variedades autóctonas. Xunta de Galicia. 231 pp. Imp. Gráficas Garabal. S.L. Santiago de Compostela.

    Google Scholar 

  • Galet, P., 1956. Cépages et vignobles de France. Tome I, Précis d'ampélographie pratique. Imp. Paul Dehan, Montpellier.

    Google Scholar 

  • Galet, P., 1958. Cépages et vignobles de France. Tomel II, Les cépages de cuve (1a partie). Imp. Paul Dehan, Montpellier.

    Google Scholar 

  • Galet, P., 1962. Cépages et vignobles de France. Tome III, Les cépages de cuve (2a partie). Imp. Le Paysan du Midi. Montpellier.

    Google Scholar 

  • Galet, P., 1967. Recherches sur les méthodes d'identification et de classification des Vitacées des zones tempérées. Thesis. Faculté des Sciences. Montpellier.

    Google Scholar 

  • Galet, P., 1990. Cépages et Vignobles de France. Tome I-II L'Ampélographie Française. 400 pp. Imp. Charles Dehan, Montpellier.

    Google Scholar 

  • Galet, P., 1993. Précis de viticulture. Imp. Charles Dehan, Montpellier.

    Google Scholar 

  • Gogorcena, Y., S. Arulsekar, A.M. Dandekar & D.E. Parfitt, 1993. Molecular markers for grape characterization. Vitis 32: 183–185.

    CAS  Google Scholar 

  • Grando, M.S., L. Demicheli, L. Biasetto & A. Scienza, 1995. RAPD markers in wild and cultivated Vitis vinifera. Vitis 34: 37–39.

    CAS  Google Scholar 

  • Grando, M.S., L. Micheli & A. Scienza, 1996. Characterization of Vitis germplasm using random amplified polymorphic DNA markers. Genet Res Crop Evol 43: 187–192.

    Article  Google Scholar 

  • Guirao, P., A. Moya & J.L. Cenis, 1995. Optimal use of random amplified polymorphic DNA in estimating the genetic relationship of four major Meloidogyne spp. Phytopathology 85: 547–551.

    CAS  Google Scholar 

  • Herrera, G.A., 1818. Agriculura general. Vol. I, 544 pp. Imprenta Real, Madrid.

    Google Scholar 

  • Hidalgo, L., 1993. Tratado de viticultura general. 983 pp. Ed Mundi-Prensa, Madrid.

    Google Scholar 

  • Huetz de Lemps, A., 1967. Vignobles et vins du Nord-Ouest de l'Espagne. Vol. I-II. Thesis Institut de Géographie. Faculté des lettres. Bordeaux.

    Google Scholar 

  • Jean-Jacques, I., A. Defontaine & J.N. Hallet, 1993. Characterization of Vitis vinifera cultivars by Random Amplified Polymorphic DNA markers. Vitis 32: 189–190.

    Google Scholar 

  • Levadoux, L., 1948. Les cépages à raisins de cuve. Prog Agric Vitic 129: 6–14.

    Google Scholar 

  • Levadoux, L., 1956. Les populations sauvages et cultivées de Vitis vinifera L. Ann. Amélior. Plantes 1: 59–118.

    Google Scholar 

  • Loureiro, M.D., M.C. Martinez; J.M. Boursiquot & P. This, 1998. Molecular marker analysis of ‘Albariño’ and some similar grapevine cultivars. J Amer Soc Hort Sci 123 (5): 842–848.

    CAS  Google Scholar 

  • Martinez, M.C., J.L: Mantilla & M.D. Loureiro, 1994. Descripción ampelográfica sobre hoja adulta de cepas de Vitis vinifera L. denominadas ‘Albariño’. Viticultura/Enologia Profesional 34: 30–40.

    Google Scholar 

  • Moreno, S., Y. Gogorcena & J.M. Ortiz, 1995. The use of RAPD markers for identification of cultivated grapevine (Vitis vinifera L.). Sci Hortic Amsterdam 62: 237–243.

    Article  CAS  Google Scholar 

  • Pérez, J.E., J. Rega & I. Orriols, 1993. Fichas técnicas de las variedades. In: Ici-Zeltia agroquímicos (Ed.), Variedades autóctonas de cepas gallegas para vinos blancos, pp. 35–64. Porriño, Pontevedra.

    Google Scholar 

  • Rohlf, F.J., 1993. NTSYS-pc. Numerical taxonomic and multivariate analysis system. Exeter software. State University of New York at Stony Brook, New York.

    Google Scholar 

  • Romesburg, H.Ch., 1984. Cluster analysis for researchers. 334 pp. Wadswoth Inc. California.

    Google Scholar 

  • Sensi, E., R. Vignani, W. Rohde & S. Biricolti, 1996. Characterization of genetic biodiversity with Vitis vinifera L. Sangiovese and Colorino genotypes by AFLP and ISTR DNA marker technology. Vitis 35: 183–188.

    Google Scholar 

  • Thomas, M.R., S. Matsumoto, P. Cain & N.S. Scott, 1993. Repetitive DNA of grapevine: classes present and sequences suitable for cultivars identification. Theor. Appl. Genet. 83: 173–180.

    Google Scholar 

  • Torres, A.M., N.F. Weeden & A. Martín, 1993. Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Theor Appl Genet 85: 937–945.

    Article  CAS  Google Scholar 

  • Tschammer, J. & E. Zyprian, 1994. Molecular characterization of grapevine cultivars of Riesling-type and of closely related Burgundies. Vitis 33: 249–250.

    CAS  Google Scholar 

  • Viala, P. & V. Vermorel, 1905. Ampélographie. Imp. Masson et Cie, Paris.

    Google Scholar 

  • Vidal, J.R., 1996. Caracterización bioquímica de las variedades blancas de vid (Vitis vinifera L) cultivadas en la Denominación de Origen Rías Baixas. Doctoral Thesis, Facultad de Biología, Universidad de Santiago de Compostela. Spain.

    Google Scholar 

  • Vidal, J.R., S. Moreno, Y. Gogorcena, A. Masa & J.M. Ortiz, 1999. On the genetic relationships and origin of six grapevine cultivars of Galicia (Spain) using RAPD markers. Am J Enol Vitic 50(1): 69–75.

    CAS  Google Scholar 

  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Misión Biológica de Galicia. Consejo Superior de Investigaciones Científicas (CSIC), P.O. Box 28, E-36080, Pontevedra, Spain

    José Ramón Vidal

  2. Centre Technique Interprofessionnel de la Vigne et du Vin (ITV France), Château de la Frémoire, F-44120, Vertou, France

    Morvan Coarer

  3. Laboratoire de Biotechnologie, UPRES of Biocatalyse, Université de Nantes, 2 rue de la Houssinière, BP 92208, F-44322, Nantes, France

    Alain Defontaine

Authors
  1. José Ramón Vidal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Morvan Coarer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alain Defontaine
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vidal, J.R., Coarer, M. & Defontaine, A. Genetic relationships among grapevine varieties grown in different French and Spanish regions based on RAPD markers. Euphytica 109, 161–172 (1999). https://doi.org/10.1023/A:1003776716394

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1003776716394

Search

Navigation