Abstract
Covalent attachment of Candida utilis cells, possibly simulating natural microbial immobilizations, stimulated stable and significant enhancement of extracellular production of alkaline protease, specifically induced by four different starvation conditions. The enzyme analysis confirmed the identity of the proteases released under all conditions of starvation and no parallel production of other proteolytic enzyme. The enhancement phenomenon as a uniform and stable effect of the whole cell immobilization is discussed in relation to the effect of multipoint, cell-solid surface contact, potentially bringing positive modulations of complex, cellular functions.
Similar content being viewed by others
References
Andrews P (1964) Estimation of molecular weights of proteins by Sephadex gel — filtration. Biochem. J. 91: 222-233
Arai M & Murao S (1977) Purification and some properties of two alkaline protease from Penicillium lilacinum. Agr. Biol. Chem. 41: 2293-2294
Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein dye binding. Anal. Biochem. 72: 248-252
Brown SC & Lucy JA (1993) Dystrophin as a mechanochemical transducer in skeletal muscle. BioEssays 15: 413-416
Costerton JW, Cheng KJ, Geesey GG, Lodd TI, Nickel JC, Dasgupta M & Marrie TJ (1987) Bacterial biofilms in nature and disease. Ann. Rev. Microbiol. 41: 435-464
Feinberg G (1976) Modified methods of dialysis. Biochem. J. 153: 721-728
Gimeno CJ, Ljungdahl PO, Styles CA & Fink GR (1992) Unipolar cell division in the yeast Saccharomyces cerevisiae lead to filamentous growth: regulation by starvation and RAS. Cell. 68: 1077-1090
Hilt W & Wolf DH (1992) Stress induced proteolysis in yeast. Mol. Microbiol. 6: 2437-2442
Jirku V (1989) Energy status of starving yeast cells immobilized by covalent linkage. Biotechnol. Lett. 11: 881-884
____ (1991a) Quantitative changes of wall polysaccharides in an immobilized yeast. Biotechnol. Appl. Biochem. 14: 388-391
____ (1991b) The effect of covalent immobilization on ethanol — induced leakage in Saccharomyces cerevisiae. Acta. Biotechnol. 11: 77-80
____ (1992a) Nystatin and killer toxin sensitivity of free and immobilized Saccharomyces cerevisiae. World J. Microbiol. Biotechnol. 8: 192-195
____ (1992b) Permeabilization of covalently immobilized Saccharomyces cerevisiae. Acta. Biotechnol. 12: 333-336
____ (1995a) Covalent immoblization as a stimulus of cell wall composition changes. Experientia 51: 569-571
____ (1995b) Novel way to study the chronological changes in yeast outer-wall proteins. World J Microbiol Biotechnol 11: 307-309
____ (1996) The use of covalently immobilized mycelia to modify the β-glucanase system of Aspergillus vesicolor. J. Chem. Tech. Biotechnol. 65: 179-185
Jones BM (1979) Regulation of contact behaviour of cells. Biol. Rev. 55: 207-235
Jones EW (1991) Free proteolytic system in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 226: 7963-7966
Jothy S, Munro SB, LeDuy L, McClure D & Blaschuk OW (1995) Adhesion or anti-adhesion in cancer: what matters more? Cancer and Metastasis Reviews 14: 363-376
Lappin-Scott HM & Costerton JW (1995) Microbial Biofilms. Cambridge University Press, Cambridge
Lazarova V & Manem J (1995) Biofilm characterization and activity analysis in water and wastewater treatment. Wat. Res. 29: 2227-2245
Mayer RJ & Doherty F (1986) Intracellular protein catabolism: state of the art. FEBS Lett. 198: 181-193
Tusker RW (1991) The effect of cell anchorage on signal transduction contributing to proliferation. Wiley-Liss, Inc., London. pp. 143-151
Willaert RG, Baron GV & De Backer L (1996) Immobilized Living Cell Systems. John Wiley & Sons Ltd, Chichester
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jirkû, V. Changes in the starvation response through covalent cell attachment. Antonie Van Leeuwenhoek 71, 369–373 (1997). https://doi.org/10.1023/A:1000235610299
Issue Date:
DOI: https://doi.org/10.1023/A:1000235610299