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An antisense coat protein gene confers immunity to potato leafroll virus in a genetically engineered potato

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Abstract

The Bzura commercial potato cultivar was transformed by sense or antisense constructs which included the coat protein gene of potato leafroll virus RNA. In the sense construct, the coat protein gene was preceded by a leader sequence shorter than that in the subgenomic RNA formed in infected cells. The antisense construct consisted of a sequence complementary to the first 2020 nucleotides of the subgenomic RNA. Selected transformants expressing viral RNA were resistant to virus challenge by viruliferous aphids. In one line, expression of the antisense RNA prevented virus infection even after grafting with scions from infected plants and therefore this transformant might be regarded as virus immune.

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References

  • Barker H, Reavy B, Kumar A, Webster KD and Mayo MA (1992) Restricted virus multiplication in potatoes transformed with the coat protein gene of potato leafroll luteovirus: similarities with a type of host gene-mediated resistance. Ann appl Biol 120: 55-64

    Google Scholar 

  • Beachy RN, Loesch-Fries S and Tumer NE (1990) Coat protein mediated resistance against virus infection. Ann Rev Phytopathol 28: 451-474

    Article  Google Scholar 

  • Cuozzo M, O'Connell KM, Kaniewski W, Fang RX, Chua N-H and Rumer NE (1988) Viral protection in transgenic tobacco plants expressing the cucumber mosaic virus coat protein or its antisense RNA. Bio/Technology 6: 549-557

    Article  Google Scholar 

  • Derrick PM and Barker H (1992) The restricted distribution of potato leafroll luteovirus antigen in potato plants with transgenic resistance resembles that in clones with one type of host gene-mediated resistance. Ann Appl Biol 120: 451-457

    Google Scholar 

  • Doyle JJ and Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue”. Phytochemical Bulletin 19: 11-15

    Google Scholar 

  • Fang G and Grumet R (1993) Genetic engineering of potyvirus resistance using constructs derived from the zuccHini yellow mosaic virus coat protein gene. Mol Plant Microbe Interact 6: 358-367

    PubMed  Google Scholar 

  • Farinelli L and Malnoe P (1993) Coat protein gene-mediated resistance to potato virus Y in tobaco: Examination of the resistance mechanism-Is the transgenic coat protein required for protection? Mol Plant Microbe Interact 6: 284-292

    PubMed  Google Scholar 

  • Greene AE and Allison RF (1994) Recombination between viral RNA and transgenic plant transcripts. Science 263: 1423-1425

    PubMed  Google Scholar 

  • Hackland AF, Rybicki EP and Thomson JA (1994) Coat protein-mediated resistance in transgenic plants. Arch Virol 139: 1-22

    PubMed  Google Scholar 

  • Hammond J and Kamo KK (1995) Effective resistance to potyvirus infection conferred by expression of antisense RNA in transgenic plants. Mol Plant Microbe Interact 8: 674-682

    PubMed  Google Scholar 

  • Huntley C and Hall TC (1993a) Minus sense transcripts of brome mosaic virus RNA-3 intercistronic region interfere with viral replication. Virology 192: 290-297

    Article  PubMed  Google Scholar 

  • Huntley C and Hall TC (1993b) Interference with bromemosaic virus replication by targeting theminus strand promoter. J Gen-Virol 74: 2445-2452

    PubMed  Google Scholar 

  • Kawchuk LM, Martin RR and Mc-Pherson J (1990) Resistance in transgenic potato expressing the potato leafroll virus coat protein gene. Mol Plant Microbe Interact 3: 301-307

    Google Scholar 

  • Kawchuk LM, Martin RR and Mc-Pherson J (1991) Sense and anti-sense RNA-mediated resistance to potato leafroll virus in Russet Burbank potato plants. Mol Plant Microbe Interact 4: 247-253

    Google Scholar 

  • Keese P., Martin RR, Kawchuk LM, Waterhouse PM and Gerlach WL (1990) Nucleotide sequences of an Australian and a Canadian isolate of potato leafroll luteovirus and their relationship with two European isolates. J GenVirol 71: 719-724

    Google Scholar 

  • Maniatis T, Frisch EF and Sambrook J (1990) Molecular Cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor New York USA

    Google Scholar 

  • Mayo MA, Robinson DJ, Jolly CA and Hyman L (1989) Nucleotide sequence of potato leafroll luteovirus RNA. J Gen Virol 70: 1037- 1051

    PubMed  Google Scholar 

  • McKinney H (1929) Mosaic diseases in the Canary Islands West Africa and Gibraltar. J Agric Res 39: 557-578

    Google Scholar 

  • Miller JS and Mayo MA (1991) The location of the 5′ end of the potato leafroll luteovirus subgenomic coat protein RNA. J Gen Virol 72: 2633-2638

    PubMed  Google Scholar 

  • Nadolska-Orczyk AL., Miłkowska A, Pałucha A, Czembor P and OrczykW(1994) Regeneration and transformation of Polish cultivars of potato. Acta Societatis Botanicorum Poloniae Vol 6 No. 4

  • Nelson A, Don AR and Johnson JD (1993) Tobacco mosaic virus infection of transgenic Nicotiana tabacumplants is inhibited by antisense constructs directed at the 50 region of viral RNA. Gene 127: 227-232

    Article  PubMed  Google Scholar 

  • Pałucha A, Sadowy E, Kujawa A, Juszczuk M., Zagórski W and Hulanicka D (1994) Nucleotide sequence of RNA of a Polish isolate of potato leafroll luteovirus Acta Bioch Polon 41: 405- 414

    Google Scholar 

  • Powell PA, Stark DM, Sanders PR and Beachy RN (1989) Protection against tobaco mosaic virus in transgenic plants that express tobacco mosaic virus antisense RNA. Proc Natl Acad Sci USA 86: 6949-6952

    PubMed  Google Scholar 

  • Rezaian MA, Skene KGM and Ellis JG (1988) Anti-sense RNAs of cucumber mosaic virus in transgenic plants assessed for control of the virus. Plant Mol Biol 11: 463-471

    Google Scholar 

  • Syller J (1985) Comparison of some isolates of potato leaf roll virus in Poland. Phytopathologische Zeitschrift 113: 17-23

    Google Scholar 

  • van den Heuvel JFJ, de Blank CM, Peters D and van Lent JWM (1995) Localisation of potato leafroll virus in leaves of secondarily-infected potato plants. Eur J Plant Pathology 101: 567-571

    Google Scholar 

  • van der Wilk F, Huisman M., Cornelissen BJC, Huttinga H and Gold-bach R (1989) Nucleotide sequence and organisation of potato leafroll virus genomic RNA. FEBS Lett 245: 51-56

    Article  PubMed  Google Scholar 

  • van der Wilk F, Posthumus-Lutke Willink D, Huisman MJ, Huttinga H and Goldbach R (1991) Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection. Plant Mol Biol 17: 431-439

    PubMed  Google Scholar 

  • Verwoerd TC, Dekker BMM and Hoekema A (1989) A small-scale procedure for the rapid isolation of plant RNAs. Nucl Acid Res 17:2632

    Google Scholar 

  • Zoeten GA (1991) Risk Assessment: Do we let history repeat itself? Phytopathology 81: 585-586

    Google Scholar 

Download references

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Authors and Affiliations

  1. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawinskiego 5a, 02-106, Warszawa, Poland

    Andrzej Pałucha, Włodzimierz Zagórski & Danuta Hulanicka

  2. Potato Research Institute, Unit Młochów, 05-832, Rozalin, Poland

    Mirosława Chrzanowska

Authors
  1. Andrzej Pałucha
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  2. Włodzimierz Zagórski
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  3. Mirosława Chrzanowska
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  4. Danuta Hulanicka
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Pałucha, A., Zagórski, W., Chrzanowska, M. et al. An antisense coat protein gene confers immunity to potato leafroll virus in a genetically engineered potato. European Journal of Plant Pathology 104, 287–293 (1998). https://doi.org/10.1023/A:1008603302807

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  • DOI: https://doi.org/10.1023/A:1008603302807

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