Abstract
A new bacterium, Citrobacter sp. Y19, catalyzing the water-gas shift reaction was isolated from an anaerobic wastewater sludge digester. It grew aerobically with a high specific growth rate of 0.7 h−1 in a mineral salt medium supplemented with yeast extract and Bacto-tryptone, and produced H2 under anaerobic conditions after the cells were transferred to tryptone-deleted medium. The maximum H2 production rate was 33 mmol H2 g−1 cell h, which was maintained for about 200 h. This is the first report on a chemoheterotrophic bacterium which utilizes CO with the production of H2 and CO2.
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References
Altschul SF, Madden TL, Schifer AJ, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25: 3389–3402.
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1989) Current Protocols in Molecular Biology. New York: John Wiley & Sons.
Barik S, Corder RE, Clausen EC, Gaddy JL (1987) Biological conversion of coal synthesis gas to methane. Energy Prog. 7: 157–160.
Bhatnagar L, Krzycki JA, Zeikus JG (1987) Analysis of hydrogen metabolism in Methanosarcina barkeri: regulation of hydrogenase and role of CO-dehydrogenase in H2 production. FEMS Microbiol. Lett. 41: 337–343.
Ferry JG (1995) CO dehydrogenase. Annu. Rev. Microbiol. 49: 305–333.
Jung GY, Jung HO, Kim JR, Ahn Y, Park S (1999) Isolation and characterization of Rhodopseudomonas palustris P4 which utilizes CO with the production of H2. Biotechnol. Lett. 21: 525–529.
Klasson KT, Lundbäck KMO, Clausen EC, Gaddy JL (1993) Kinetics of light limited growth and biological hydrogen production from carbon monoxide and water by Rhodospirillum rubrum. J. Biotechnol. 29: 177–188.
Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M, eds. Nucleic Acid Techniques in Bacterial Systematics. New York: John Wiley & Sons, pp. 115–147.
Sakazaki R (1984) Genus IV. Citrobacter Werkman and Gillen 1932, 173AL. In: Krieg NR, ed. Bergey's Manual of Systematic Bacteriology, Vol. 1. Baltimore: Williams & Wilkins, pp. 458–461.
Shin YK, Lee J-S, Chun CO, Kim H-J, Park Y-H (1996) Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J. Mirobiol. Biotechnol. 6: 68–69.
Tanner RS, Miller LM, Yang D (1993) Clostridium ljungdahlii sp. nov., an acetogenic species in clostridial rRNA homology group I. Int. J. Syst. Bacteriol. 43: 232–236.
Uffen RL (1976) Anaerobic growth of a Rhodopseudomonas species in the dark with carbon monoxide as sole carbon and energy substrate. Proc. Natl. Acad. Sci. USA 73: 3298–3302.
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Yeol Jung, G., Rae Kim, J., Ok Jung, H. et al. A new chemoheterotrophic bacterium catalyzing water-gas shift reaction. Biotechnology Letters 21, 869–873 (1999). https://doi.org/10.1023/A:1005599600510
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DOI: https://doi.org/10.1023/A:1005599600510