Biotechnology Letters

, Volume 26, Issue 11, pp 933–937 | Cite as

Flow process for electroextraction of intracellular enzymes from the fission yeast, Schizosaccharomyces pombe

  • V. Ganeva
  • B. Galutzov
  • J. Teissié


Flow treatment of the yeast, Schizosaccharomyces pombe, with high intensity electric field pulses released intracellular enzymes such as glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase. Over 70% of the total activity was liberated within 4 h after pulse application. The optimal field intensities were considerably higher than that needed for irreversible plasma membrane permeabilization.

cytoplasmic enzyme electro extraction electropulsation flow process Schizosaccharomyces pombe 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • V. Ganeva
    • 1
  • B. Galutzov
    • 1
  • J. Teissié
    • 2
  1. 1.Faculty of BiologyUniversity of SofiaSofiaBulgaria
  2. 2.IPBS CNRS UMR5089Toulouse cédex 4France

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