Biotechnology Letters

, Volume 26, Issue 11, pp 923–927 | Cite as

Production of an anti-complement exo-polymer produced by Auricularia auricula-judae in submerged culture

  • Sang-Chul Jeong
  • Sung-Pill Cho
  • Byung-Keun Yang
  • Young-Ah Gu
  • Jin-Ho Jang
  • Tae-Lin Huh
  • Chi-Hyun Song

Abstract

Exo-polymer (EP) was produced at 1.2 g l−1 in submerged culture of Auricular auricula-judae. Crude EP (AJ-0) has 70% anti-complementary activity (inhibition of total complementary hemolysis 50%; ITCH50). The activating pathway of the complement system occurred through both the classical and alternative pathways, though the major pathway was the classical one. Fractionation of AJ-0 using Sepharose CL-6B gel chromatography gave three major fractions (AJ-Fr-I, II and III) of which the first was the most active. The mycelial growth and EP production of A. auricula-judae were optimal at pH 6, 25 °C and pH 5, 25 °C, respectively.

anti-complementary activity Auricularia auricula-judae exo-polymer 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Sang-Chul Jeong
    • 1
  • Sung-Pill Cho
    • 1
  • Byung-Keun Yang
    • 2
  • Young-Ah Gu
    • 1
  • Jin-Ho Jang
    • 2
  • Tae-Lin Huh
    • 3
  • Chi-Hyun Song
    • 1
  1. 1.Department of BiotechnologyDaegu UniversityGyungsan, GyungbukSouth Korea
  2. 2.Research Center for Processing & Application of Agricultural ProductsDaegu UniversityGyungsan, GyungbukSouth Korea
  3. 3.TG BiotechKyungpook National UniversityDaeguSouth Korea

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