Biotechnology Letters

, Volume 26, Issue 11, pp 911–915

1,3-Propanediol production by Klebsiella pneumoniae under different aeration strategies

  • Ke-Ke Cheng
  • De-Hua Liu
  • Yan Sun
  • Wei-Bin Liu
Article

Abstract

1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l−1 and 16 g 2,3-butanediol l−1 with total diol yield of 0.6 mol−1 glycerol.

aeration fermentation glycerol Klebsiella pneumoniae 1,3-propanediol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Biebl H, Menzel K, Zeng AP, Deckwer WD (1999) Microbial production of 1,3-propanediol. Appl. Microbiol. Biotechnol. 52: 289–297.Google Scholar
  2. Biebl H, Zeng AP, Menzel K, Deckwer WD (1998) Fermentation of glycerol to 1,3-propanediol and 2,3-butanediol by Klebsiella pneumoniae. Appl. Microbiol. Biotechnol. 50: 24–29.Google Scholar
  3. Chen Xi, Xiu Zhilong, Wang Jianfeng, Zheng Daijia, Xu Ping (2003) Stoichiometrc analysis and experimental investigation of glycerol bioconversion to 1,3-propanediol by Klebsiella pneumoniae under microaerobic conditions. Enzyme Microb. Technol. 33: 386–394.Google Scholar
  4. El-Ziney MG, Arneborg N, Uyttendaele M, Debevere J, Jakobsen M (1998) Characterization of growth and metabolite production of Lactobacillus reuteri during glucose/glycerol cofermentation in batch and continuous cultures. Biotechnol. Lett. 20: 913–916.Google Scholar
  5. Huang H, Gong CS, Tsao GT (2002) Production of 1,3-propanediol by Klebsiella pneumoniae. Appl. Biochem. Biotechnol. 98: 687–698.Google Scholar
  6. Menzel K, Ahrens K, Zeng AP, Deckwer WD (1998) Kinetic, dynamic, and pathway studies of glycerol metabolism by Klebsiella pneumoniae in anaerobic continuous culture: IV. Enzymes and fluxes of pyruvate metabolism. Biotechnol. Bioeng. 60: 617–626.Google Scholar
  7. Reimann A, Biebl H, Deckwer WD (1998) Production of 1,3-propanediol by Clostridium butyricum in continuous culture with cell recycling. Appl. Microbiol. Biotechnol. 49: 359–363.Google Scholar
  8. Zeng AP, Biebl H, Schlieker H, Deckwer WD (1993) Pathway analysis of glycerol fermentation by Klebsiella pneumoniae: regulation of reducing equivalent balance and product formation. Enzyme Microb. Technol. 9: 770–779.Google Scholar
  9. Zeng AP, Ross A, Bieble H, Tag C (1994) Multiple Product inhibition and growth modeling of Clostridium butyricum and Klebsiella pneumoniae in glycerol fermentation. Biotechnol. Bioeng. 44: 902–911.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Ke-Ke Cheng
    • 1
  • De-Hua Liu
    • 1
  • Yan Sun
    • 1
  • Wei-Bin Liu
    • 1
  1. 1.Department of Chemical EngineeringTsinghua UniversityBeijingP.R. China

Personalised recommendations