Effects of biosurfactants produced by Candida antarctica on the biodegradation of petroleum compounds

Article

Abstract

The effects of biosurfactants on the biodegradation of petroleum compounds were investigated. Candida antarctica T-34 could produce extracellular biosurfactant mannosylerythritol lipids (MELs) when it was cultured in vegetable oil. In addition, in our previous study, it was found that this strain could also produce a new type of biosurfactant while it grew on n-undecane (C11H24), and the biosurfactant was named as BS-UC. In flask culture of Candida antarctica, the addition of BS-UC could improve the biodegradation rate of some n-alkanes (e.g. 90.2% for n-decane, 90.2% for n-undecane, 89.0% for dodecane), a mixture of n-alkanes (82.3%) and kerosene (72.5%). By comparing the effects of the biosurfactants BS-UC and MEL and chemical surfactants on the biodegradation of crude oil, it was found that biosurfactants could be used to enhance the degradation of petroleum compounds instead of chemical surfactants. In a laboratory scale immobilized bioreactor, the addition of biosurfactant improved not only the emulsification of kerosene in simulated wastewater but also its biodegradation rate. The highest degradation rate of kerosene by addition of MEL and BS-UC reached 87 and 90% at 15 h, respectively. The results showed that the biosurfactant BS-UC was highly promising for work on biodegradation of hydrophobic contaminants.

Biodegradation biosurfactant Candida antarctica kerosene mannosylerythritol lipid n-alkanes petroleum compounds 

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References

  1. Bai, G.Y., Brusseau, M.L. & Miller, R.M. 1998 Influence of cation type, ionic strength, and pH on solubilization and mobilization of residual hydrocarbon by a biosurfactant. Journal of Contaminant Hydrology 30, 265–279.Google Scholar
  2. Baldi, F., Ivosevic, N., Minaui, A., Pepi, M., Fani, R., Svetlicii, V. & Zutii, V. 1999 Adhersion of Acinetobacter venetians to diesel fuel droplets studied with in situ electrochemical and molecular probes. Applied and Environmental Microbiology 65, 2041–2048.Google Scholar
  3. Banat, I.M., Makkar, R.S. & Cameotra, S.S. 2000 Microbial production of surfactants and their commercial potential. Applied Microbiology and Biotechnology 53, 495–508.Google Scholar
  4. Barathi, S. & Vasudevan, N. 2001 Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from a petroleumcontaminated soil. Environment International 26, 413–416.Google Scholar
  5. Hua, Z.Z., Chen, J. & Lun, S.Y. 1998 Production of biosurfactant and degradation of n-alkanes by Candida antarctica. Journal of Nanjing University 34, 149–154.Google Scholar
  6. Hua, Z.Z., Chen, J. & Lun, S.Y. 1999 Isolation and properties of new type biosurfactant mannosylerythritol lipids. Chinese Journal of Natural Product Research and Development 11, 11–15.Google Scholar
  7. Koch, A.K., Kappeli, O., Fiechter, A. & Reiser, J. 1991 Hydrocarbon assimilation and biosurfactant production in Pseudomonas aeruginosa mutants. Journal of Bacteriology 173, 4212–4219.Google Scholar
  8. Leahy, J.G. & Colwell, R.R. 1990 Microbial degradation of hydrocarbon in the environment. Applied and Environmental Microbiology 54, 305–315.Google Scholar
  9. Neu, T.R. 1996 Significance of bacterial surface-active compounds in interaction of bacteria with interfaces. Microbiological Reviews 60, 151–166.Google Scholar
  10. Providenti, M.A., Flemming, C.A., Lee, H. & Trevors, J.T. 1995 Effect of addition of rhamnolipid biosurfactants or rhamnolipid producing Pseudomonas aeruginosa on phenanthrene mineralization in soil slurries. FEMS Microbiology Ecology 17, 15–26.Google Scholar
  11. Walter, M.V., Nelson, E.C., Firmstone, G., Martin, D.G., Clayton, M.J., Simpson, S. & Spaulding, S. 1997 Surfactant enhances biodegradation of hydrocarbons: Microcosm and field study. Journal of Soil Contamination 6, 61–77.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Zhaozhe Hua
    • 1
  • Yan Chen
    • 1
  • Guocheng Du
    • 1
  • Jian Chen
    • 1
    • 2
  1. 1.School of BiotechnologySouthern Yangtze UniversityWuxiP.R. China
  2. 2.Key laboratory of Industrial BiotechnologyMinistry of Education, Southern Yangtze UniversityWuxiP.R. China

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