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Wetlands Ecology and Management

, Volume 11, Issue 5, pp 317–330 | Cite as

Nitrogen dynamics in seasonally flooded soils in the Amazon floodplain

  • Matthias Koschorreck
  • Assad Darwich
Article

Abstract

Large areas of the Amazon are subject to seasonal flooding due to water level changes of the river. This ‘flood pulse’ causes rapidly changing conditions for microorganisms living in the soils which affects the cycling of nitrogen in the ecosystem. An understanding of the nitrogen dynamics in the seasonally flooded soils is essential for the development of productive and sustainable management concepts. We measured nitrogen concentrations, denitrifier enzyme activity (DEA), cell numbers of nitrifying and denitrifying bacteria, respiration, pH and total carbon in the seasonally flooded soils over one entire annual hydrological cycle. By comparing three sites with different vegetation (forest, aquatic macrophyte stand and bare sediment with annual herbs) we assessed the effect of vegetation on soil nitrogen dynamics. Inorganic nitrogen was always dominated by ammonium indicating reduced conditions in the soil even during the terrestrial phase. Although conditions were generally poor for nitrification we observed high numbers of nitrifying bacteria between 104 and 107cells g−1. Pulses of ammonium as well as high DEA were observed during the transition periods between aquatic and terrestrial phase. Thus the alternation between aquatic and terrestrial phase promotes nitrogen mineralization and denitrification in the soils. There were no plausible correlations between microbial activities and numbers with soil physical or chemical parameters except a relation between the numbers of nitrate reducing bacteria and soil moisture (R2 = 0.81) and ammonium (R2 = 0.92) at one site. This shows the complex regulation patterns in this habitat. Different vegetation did not alter the general patterns of nitrogen dynamics but the absolute extend of fluctuations. We conclude that both the soil physical and chemical changes directly caused by the flood pulse and the vegetation have a great impact on microbial nitrogen turnover in the soils. The effects of the flood pulse can be buffered by a fine soil texture or a litter layer which prevents desiccation of the soil during the terrestrial phase.

Amazon Flood pulse Floodplain Microbial ecology Nitrogen 

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References

  1. Achtnich C., Bak F. and Conrad R. 1995. Competition for electron donors among nitrate reducers, ferric iron reducers, sulfate reducers and methanogens in anoxic paddy soil. Biology and Fertility of Soils 19: 65–72.Google Scholar
  2. Alef K. and Nannipieri P. 1995. Methods in Applied Soil Micro-biology and Biochemistry. Academic Press, London, GB, UK.Google Scholar
  3. Aulak M.S., Kuplid-Singh, Bijay-Singh and Doran J.W. 1996. Kinetics of nitrification under upland and flooded soils of varying texture. Communications in Soil Science and Plant Analysis 27(9110): 2079–2089.Google Scholar
  4. Barrios E. and Herrera R. 1994. Nitrogen cycling in a Venezuelan tropical seasonally flooded forest: soil nitrogen mineralization and nitrification. Journal of Tropical Ecology 10: 399–416.Google Scholar
  5. Baumgartner M. and Conrad R. 1992. Effects of soil variables and season on the production and consumption of nitric oxide in oxic soils. Biology and Fertility of Soils 14: 166–174.Google Scholar
  6. Bossio D.A. and Scow K.M. 1998. Impacts of carbon and flooding on soil microbial communities: phospholipid fatty acid profiles and substrate utilisation pattern. Microbial Ecology 35: 265–278.Google Scholar
  7. Both G.J., Gerads S. and Laanbroek H.J. 1990a. Most probable numbers of chemolithoautotrophic nitrite-oxidizing bacteria in well drained grassland soils: stimulation by high nitrite conthe centrations. FEMS Microbiology Ecology 74: 287–294.Google Scholar
  8. Brune A., Frenzel P. and Cypionka H. 2000. Life at the oxic-anoxic interface: microbial activities and adaptations. FEMS Microbiology Reviews 24: 691–710.Google Scholar
  9. Davidsson T.E. and Leonardson L. 1996. Effect of nitrate and organic carbon additions on the denitrification in two artificially flooded soils. Ecological Engineering 7: 139–149.Google Scholar
  10. Davidsson T.E. and Leonardson L. 1997. Production of nitrous oxide in artificially flooded and drained soil. Wetlands Ecology and Management 5: 111–119.Google Scholar
  11. Dendooven L., Duchateau L. and Anderson I. 1996. Gaseous products of the denitrification process as affected by the antece-dent water regime of the soil. Soil Biology and Biochemistry 28: 239–245.Google Scholar
  12. Forsberg B. 1984. Nutrient processing in Amazon floodplain lakes. Verhandlungen der Internationalen Vereinigung der Limnologie 22: 1294–1298.Google Scholar
  13. Furch K. and Junk W.J. 1992. Nutrient dynamics of submersed decomposing Amazonian herbaceous plant species Paspalum fasciculatum and Echinochloa polystachya. Rev. Hydrobiol. Trop. 25: 75–85.Google Scholar
  14. Furch K. 1997. Chemistry of Varzea and Igapo soils and nutrient inventory of their floodplain forests. In: Junk W.J. (ed.), The Central Amazon Floodplain – Ecology of a Pulsing System. Springer-Verlag, Berlin, pp. 47–67.Google Scholar
  15. Furch K. and Junk W.J. 1997. The chemical composition, food value, and decomposition of herbaceous plants, leaves, and leaf litter of the floodplain forest. In: Junk W.J. (ed.), The Central Amazon Floodplain – Ecology of a Pulsing System. Springer-Verlag, Berlin, pp. 187–207.Google Scholar
  16. Grasshoff K. 1976. Methods of Seawater Analysis.Verlag Chemie, Weinheim.Google Scholar
  17. Henckel T. and Conrad R. 1998. Characterisation of microbial NO production, N O production and CH oxidation initiated by 2 4 aeration of anoxic rice field soil. Biogeochemistry 40: 17–36.Google Scholar
  18. Howard-Williams C. and Junk W.J. 1976. The decomposition of aquatic macrophytes in the floating meadows of a Central ´ Amazonian varzea lake. Biogeographica 7: 115–123.Google Scholar
  19. Junk W.J., Bayley P.B. and Sparks R.E. 1989. The flood pulse concept in river-flood-plain systems. In: Dodge D.P. (ed.), Proceedings of the International Large River symphosium, 106. Can. Spec. Publ. Fish. Aquat. Sci., pp. 110–127.Google Scholar
  20. Junk W.J. (ed.) 1997. The Central Amazon Floodplain – Ecology of a Pulsing System. Springer-Verlag, Berlin.Google Scholar
  21. Junk W.J. and Piedade M.T.F. 1997. Plant life in the floodplain with special reference to herbaceos plants. In: Junk W.J. (ed.), The Central Amazon Floodplain – Ecology of a Pulsing System. Springer-Verlag, Berlin, pp. 147–186.Google Scholar
  22. Kern J. 1995. Die Bedeutung der N2-Fixierung und der Denitrifika-tion fur den Stickstoffhaushalt des amazonischen Uberschwem-mungssees Lago Camaleao, PhD thesis, Hamburg.Google Scholar
  23. Kern J., Darwich A., Furch K. and Junk W.J. 1996. Seasonal denitrification in flooded and exposed sediments from the Amazon floodplain at Lago Camaleao. Microbial Ecology 32: 47–57.Google Scholar
  24. Kieft T.L., Soroker E. and Firestone M.K. 1987. Microbial biomass response to a rapid increase in water potential when dry soil is wetted. Soil Biology and Biochemistry 19: 119–126.Google Scholar
  25. Kluber H.D. and Conrad R. 1998. Effects of nitrate, nitrite, NO and N O on methanogenesis and other redox processes in anoxic rice 2 field soil. FEMS Microbiology Ecology 25: 301–318.Google Scholar
  26. Koschorreck M. and Conrad R. 1997. Kinetics of nitric oxide mostconsumption in tropical soils under oxic and anoxic conditions. Biology and Fertility of Soils 25: 82–88.Google Scholar
  27. Lorch H.J., Benckieser G. and Ottow J.C.G. 1995. Basic methods for counting microorganisms in soil and water. In: Alef K. and Nannipieri P. (eds), Methods in Applied Soil Microbiology and Biochemistry. Academic Press, London, pp. 146–161.Google Scholar
  28. Meyer U. 1991. Feinwurzelsysteme und Mykorrhizatypen als An-¨ passungsmechanismen in Zentralamazonischen Uberschwem-¨ mungswaldern – Igapo und Varzea, PhD thesis, Hohenheim.Google Scholar
  29. Morison J.I.L., Piedade M.T.F., Muller E., Long S.P., Junk W.J. and Jones M.B. 2000.Very high productivity of the C aquatic grass 4 Echinochloa polystachya in the Amazon floodplain confirmed by net ecosystem CO2 flux measurements. Oecologia 125: 400–411.Google Scholar
  30. Neill C.H. 1995. Seasonal flooding, nitrogen mineralization and nitrogen utilisation in a prairie marsh. Biogeochemistry 30: 171–189.Google Scholar
  31. Nijburg J.W. and Laanbroek H.J. 1997. The influence of Glyceria maxima and nitrate input on the composition and nitrate metabo-lism of the dissimilatory nitrate-reducing bacterial community. FEMS Microbiology Ecology 22: 57–63.Google Scholar
  32. Nishio T. and Fujimoto T. 1991. Remineralization of nitrogen immobilised by soil microorganisms and effect of drying and rewetting of soils. Soil Science and Plant Nutrition 37: 351–355.Google Scholar
  33. Orchard V.A. and Cook F.J. 1983. Relationship between soil respiration and soil moisture. Soil Biology and Biochemistry 15: 447–453.Google Scholar
  34. Pavel E.W., Reneau R.B., Berry D.F., Smith E.P. and Mostaghimi S. 1996. Denitrification potential of nontidal riparian wetland soils in Virginia coastal plain. Water Research 30: 2798–2804.Google Scholar
  35. Pell M., Stenberg B., Stenstrom J. and Torstensson L. 1996. Potential denitrification activity assay in soil – with or without chloramphenicol. Soil Biology and Biochemistry 28: 393–398.Google Scholar
  36. Pfenning K.S. and McMahon P.B. 1996. Effect of nitrate, organic carbon, and temperature on potential denitrification rates in nitrate-rich riverbed systems. Journal of Hydrology 187: 283–295.Google Scholar
  37. Piedade M.T.F., Junk W.J. and Long S.P. 1991. The productivity of the C4 grass Echinochloa polystachya on the Amazon floodplain. Ecology 72: 1456–1463.Google Scholar
  38. Ponnamperuma F.N. 1984. Effects of flooding on soils. In: Kozlow-ski T.T. (ed.), Flooding and Plant GrowthVol. 2. Academic Press, Orlando, pp. 9–45.Google Scholar
  39. Qiu S. and McComb A.J. 1996. Drying-induced stimulation of ammonium release and nitrification in reflooded lake sediment. Marine and Freshwater Research 47: 531–536.Google Scholar
  40. Raich J.W. and Schlesinger W.H. 1992. The global carbon dioxide flux in soil respiration and its relationship to vegetation and climate. Tellus 44B: 81–99.Google Scholar
  41. Reddy K.R. and Patrick W.H. Jr 1975. Effect of alternate aerobic and anaerobic conditions on redox potential, organic matter decomposition and nitrogen loss in a flooded soil. Soil Biology and Biochemistry 7: 87–94.Google Scholar
  42. Reddy K.R. 1982. Nitrogen cycling in a flooded-soil ecosystem planted to rice (Oryza sativa L.). Plant and Soil 67: 209–220.Google Scholar
  43. Robertson G.P. 1989. Nitrification and denitrification in humid tropical ecosystems: potential controls on nitrogen retention. In: Proctor J. (ed.), Mineral Nutrients in Tropical Forest and Savanna Ecosystems. Blackwell Scientific, Boston, pp. 55–69.Google Scholar
  44. Rowe R., Todd R. and Waide J. 1977. Microtechnique for mostconsumption probable-number analysis. Applied and Environmental Microbiology 33: 675–680.Google Scholar
  45. Sand-Jensen K., Prahl C. and Stockholm H. 1982. Oxygen release from roots of submerged aquatic macrophytes. OIKOS 38: 349–354.Google Scholar
  46. Schlichting E. and Blume H.O. 1966. Bodenkundliches Praktikum. Paul Parey, Hamburg.Google Scholar
  47. Setaro F.V. and Melack J.M. 1984. Response of phytoplankton to experimental nutrient enrichment in an Amazon floodplain lake. Limnology Oceanography 29: 972–984.Google Scholar
  48. Sloth N.P., Blackburn H., Hansen L.S., Risgaard-Petersen N. and Lomstein B.A. 1995. Nitrogen cycling in sediments with differnet ent organic loading. Marine Ecology Progress Series 116: 163–170.Google Scholar
  49. Smith C.J. and Patrick W.H. Jr 1983. Nitrous oxide emission as affected by alternate anaerobic and aerobic conditions from soil suspensions enriched with ammonia sulfate. Soil Biology and Biochemistry 15: 693–697.Google Scholar
  50. Tiedje J.M. 1982. Denitrification. In: Page A.L., Miller R.H. and Kenney D.R. (eds), Methods of Soil Analysis Vol. 2. American Society of Agronomy, Madison, WI, USA, pp. 1011–1026.Google Scholar
  51. Tiedje J.M. 1988. Ecology of denitrification and dissimilatory nitrate reduction to ammonium. In: Zehnder A.J.B. (ed.), Biology of Anaerobic Microorganisms. Wiley, New York, pp. 179–244.Google Scholar
  52. Tietma A., Warmerdam B., Lenting E. and Riemer L. 1992. Abiotic factors regulating nitrogen transformations in the organic layer of acid forest soils: moisture and pH. Plant and Soil 147: 69–78.Google Scholar
  53. Van Gestel M., Merckx R. and Vlassak K. 1993. Microbial biomass responses to soil drying and rewetting – the fate of fast-growing and slow-growing microorganisms in oils from different cli-mates. Soil Biology and Biochemistry 25: 109–123.Google Scholar
  54. Wassmann R. and Martius C. 1997. Methane emissions from the Amazon floodplain. In: Junk W.J. (ed.), The Central Amazon Floodplain. Springer-Verlag, Berlin, pp. 137–143.Google Scholar
  55. West A.W., Sparling G.P. and Speir T.W. 1989. Microbial activity in gradually dried or rewetted soils as governed by water and substrate availability. Australian Journal of Soil Research 27: 747–757.Google Scholar
  56. Worbes M. 1986. Lebensbedingungen und Holzwachstum in ¨ ¨ Zentralamazonischen Uberschwemmungswaldern. In Scripta Geobotanica XVII.Google Scholar
  57. Worbes M. 1997. The forest ecosystem of the floodplains. In: Junk W.J. (ed.), The Central Amazon Floodplain – Ecology of a Pulsing System. Springer, Berlin, pp. 223–266.Google Scholar
  58. Zaret T.M., Devol A.H. and Dos Santos A. 1981. Nutrient addition experiments in Lago Jacaretinga, Central Amazon basin, Brasil. Verhandlungen der Internationalen Vereinigung fur Limnologie 21: 721–724.Google Scholar

Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  1. 1.Department of Inland Water ResearchUFZ – Centre for Environmental Research Leipzig-Halle GmbHMagdeburgGermany
  2. 2.Instituto Nacional de Pesquisas da Amazônia (INPA)ManausBrazil

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