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Veterinary Research Communications

, Volume 28, Issue 8, pp 657–667 | Cite as

Prevalent Serotypes of Pasteurella multocida Isolated from Different Animal and Avian Species in India

  • A.A. Kumar
  • S.B. Shivachandra
  • A. Biswas
  • V.P. Singh
  • Vijendra P. Singh
  • S.K. Srivastava
Article

Abstract

Identification and estimation of the prevalence ofPasteurella multocida organisms in different animal and avian species in India during November 2000 to July 2003 was carried out. Out of 418 samples collected from different outbreaks suspected to be caused byP. multocida, a total of 206 bacterial cultures were identified asP. multocida on the basis of cultural, morphological and biochemical characteristics. All the 206 cultures were isolated from different domestic animal species (cattle, buffalo, sheep, goat, pig and rabbit), avian species (chicken, duck, quail, turkey, goose) and wild animals such as leopard and deer. Serotyping ofP. multocida cultures revealed the presence of various serotypes (A:1, A:3, A:1,3, A:4, B:2, D:1and –:1) among the livestock population. P. multocida polymerase chain reaction (PCR) assay applied on different forms of bacterial cultures (bacterial culture lysate, direct bacterial colony and mixed bacterial culture lysate) yielded an amplified product of ∼460 bp specific forP. multocida. The results of PCR assay correlated well with conventional methods of identification. The present investigation revealed the presence of varied serotypes among livestock and PCR assay was found to be useful for rapid, sensitive and specific diagnosis of pasteurellosis in animals and avian species.

animals avians Pasteurella multocida PCR serotypes 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • A.A. Kumar
    • 1
  • S.B. Shivachandra
    • 1
  • A. Biswas
    • 1
  • V.P. Singh
    • 1
  • Vijendra P. Singh
    • 1
  • S.K. Srivastava
    • 1
  1. 1.Division of Bacteriology and MycologyIndian Veterinary Research InstituteIzatnagarU.P. India

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