Tropical Animal Health and Production

, Volume 36, Issue 4, pp 307–320 | Cite as

A Classical Live Attenuated Vaccine for Sheep Pox

  • V. Bhanuprakash
  • B.K. Indrani
  • R. Hegde
  • M.M. Kumar
  • A.R.S. Moorthy
Article

Abstract

A classical live attenuated sheep pox vaccine was prepared using the Ranipet strain of sheep pox virus (SPV) at the 50th passage in a secondary lamb testicular cell system. The TCID50 and RD50 were 109.63/ml and 109.51/ml, respectively. The SID50 of SPV challenge virus was 10/ml. The vaccine was found to have no adverse effects in laboratory animals, and was safe and effective in SPV seronegative lambs. In the field, 660 sheep were vaccinated with an immunizing dose containing 1×10 TCID50. Randomly selected vaccinated sheep mounted good cell-mediated immunity and humoral responses as measured by glucose utilization test and serum neutralization test, respectively, for the study period of 6 months.

attenuated virus Ranipet strain sheep pox vaccine 

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REFERENCES

  1. Anon., 1998–1999. Report on integrated sample survey for estimation of production of milk, eggs, wool and meat for the year 1998–99. Annual Report, (Directorate of Animal Husbandry and Veterinary Services, Karnataka)Google Scholar
  2. Awati, B., 1992. Safety and potency tests of Kenyan strain of sheep pox virus vaccine in goats, (MVSc thesis, University of Agricultural Sciences, Bangalore, Karnataka, India)Google Scholar
  3. Carn, V.M., 1993. Control of capripox virus infections. Vaccine, 11, 1275-1279Google Scholar
  4. Hegde, R., 1988. Studies on antigenic analysis of Ranipet strain of sheep pox virus, (MVSc thesis, University of Agricultural Sciences, Bangalore, Karnataka, India)Google Scholar
  5. Jassim, F.A., 1979. Biological properties of Ranipet strain of sheep pox virus, (MVSc thesis, University of Agricultural Sciences, Bangalore, Karnataka, India)Google Scholar
  6. Jassim, F.A. and Hawa, L.H., 1981. Adaptation and attenuation of local strain of sheep pox virus in lamb testicle monolayers system. Indian Veterinary Journal, 58, 757-762Google Scholar
  7. Kalpana, G., 1993. Evaluation of immunity conferred by Kenyan strain of Capripox virus against goatpox infection and the antigenic relationship between the two viruses, (MVSc thesis, University of Agricultural Sciences, Bangalore, Karnataka, India)Google Scholar
  8. Martin, W.B., Ergin, H. and Koylu, A., 1973. Tests in sheep of attenuated sheep pox vaccines. Research in Veterinary Science, 14, 53-61Google Scholar
  9. Pandey, A.K. and Nanda, Y.P., 1988. Laboratory Manual for Tissue Culture Sheep Pox Vaccine Production, (IVRI, Izatnagar, UP, India)Google Scholar
  10. Rafyi, A. and Chamsy, M.H., 1956. Seven years' control of sheep pox in Iran with an adsorbed tissue vaccine on aluminium gel. British Veterinary Journal, 112, 541-547Google Scholar
  11. Ramesh, K.G., 1980. Immunological studies on the Ranipet strain of sheep pox virus propagated in lamb testes cell culture, (MVSc thesis, University of Agricultural Sciences, Bangalore, Karnataka, India)Google Scholar
  12. Ramyar, H., 1965. Studies on the immunogenic properties of tissue culture sheep pox virus. Zentallblatt für Veterinarmedizin, 12B, 537-540Google Scholar
  13. Ramyar, H. and Hessami, M., 1970. Studies on the duration of immunity conferred by a live modified sheep pox tissue culture virus vaccine. Zentralblatt für Veterinarmedizin, 17B, 869-874Google Scholar
  14. Reed, L.J. and Muench, H., 1938. Simple method of estimating 50% end points. American Journal of Hygiene, 27, 493-497Google Scholar
  15. Sathe, R.G., 1931. Pox in sheep. Indian Veterinary Journal, 8, 118-121Google Scholar
  16. Shome, B.R., 1988. Comparative biochemical and immunological studies on polypeptides of field and cell culture adapted Ranipet isolate of sheep pox virus, (MVSc thesis, University of Agricultural Sciences, Bangalore, Karnataka, India)Google Scholar
  17. Snedecor, G.W. and Cochran, A.W., 1967. Statistical Methods, (IBH Publications, Calcutta)Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • V. Bhanuprakash
    • 1
  • B.K. Indrani
    • 2
  • R. Hegde
    • 3
  • M.M. Kumar
    • 3
  • A.R.S. Moorthy
    • 4
  1. 1.ERV and Clinical Virology Laboratories, Division of VirologyIVRI, MukteshwarUttaranchalIndia
  2. 2.Rallis Research CentreBangalore, KarnatakaIndia
  3. 3.Institute of Animal Health and Veterinary BiologicalsBangalore, KarnatakaIndia
  4. 4.Department of Veterinary Microbiology, Veterinary CollegeUniversity of Agricultural SciencesBangalore, KarnatakaIndia

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