Plant Cell, Tissue and Organ Culture

, Volume 77, Issue 3, pp 221–230 | Cite as

Micropropagation of Ranunculus Asiaticus: A Review and Perspectives

  • Margherita BerutoEmail author
  • Pierre Debergh


Ranunculus asiaticus is an important ornamental species mainly cultivated in the countries surrounding the Mediterranean sea. So far the multiplication of this plant has been mainly carried out by seed and rhizome division; however, these systems present many drawbacks. Tissue culture is an attractive alternative for accelerated propagation of selected and indexed genotypes. In this paper we review the work carried out on in vitro culture of R. asiaticus and present a flow chart for its commercial production, using axillary budding and embryogenesis. Although the price of micropropagated plants is higher compared to traditional material (seedlings and rhizomes from seed populations), it should be considered that tissue cultured plants have a better rhizome yield per plant; moreover, the tissue culture approach allows to offer clonal material of selected lines.

axillary bud stimulation commercial production micropropagation Ranunculus asiaticus L. somatic embryogenesis 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Istituto Regionale per la FloricolturaSanremo (IM)Italy
  2. 2.Department of Plant Sciences, Laboratory of Horticulture, Faculty of Agricultural and Applied Biological SciencesUniversity GentGentBelgium

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