Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 77, Issue 3, pp 221–230 | Cite as

Micropropagation of Ranunculus Asiaticus: A Review and Perspectives

  • Margherita BerutoEmail author
  • Pierre Debergh
Article

Abstract

Ranunculus asiaticus is an important ornamental species mainly cultivated in the countries surrounding the Mediterranean sea. So far the multiplication of this plant has been mainly carried out by seed and rhizome division; however, these systems present many drawbacks. Tissue culture is an attractive alternative for accelerated propagation of selected and indexed genotypes. In this paper we review the work carried out on in vitro culture of R. asiaticus and present a flow chart for its commercial production, using axillary budding and embryogenesis. Although the price of micropropagated plants is higher compared to traditional material (seedlings and rhizomes from seed populations), it should be considered that tissue cultured plants have a better rhizome yield per plant; moreover, the tissue culture approach allows to offer clonal material of selected lines.

axillary bud stimulation commercial production micropropagation Ranunculus asiaticus L. somatic embryogenesis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beruto M (1997) Agar and gel characteristics with special reference to micropropagation systems of Ranunculus asiaticu L. PhD Thesis, Gent, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, University Gent, Belgium, pp. 21–44, 125-184Google Scholar
  2. Beruto M & Debergh P (1992) Somatic embryogenesis in Ranunculus asiaticus L. hybr. thalamus cultivated in vitro. Plant Cell Tiss. Org. Cult. 29: 161–165Google Scholar
  3. Beruto M & Portogallo C (2000) Factors affecting the growth of Ranunculus in vitro. Acta Hort. 520: 163–170Google Scholar
  4. Beruto M, Siccardi G & Lercari G (1989) Risultati preliminari nella propagazione in vitro del ranuncolo. In: Giaggioli G (ed) Proceedings of Symposium on 'Colture in vitro emicropropagazione in ortoflorofrutticoltura' Abstracts (p. 33). Cesena, Italy. Lo Scarabeo Press, BolognaGoogle Scholar
  5. Beruto M, Cane G & Debergh P (1996a) Field performance of tissue-cultured plants of Ranunculus asiaticus L. Sci. Hortic. 66: 229–239Google Scholar
  6. Beruto M, Curir P & Debergh P (1996b) Callus growth and somatic embryogenesis in thalamus tissue of Ranunculus asiaticus L. cultivated in vitro: cytokinin effect and phenol metabolism. In Vitro Cell. Dev. Biol. Plant 32: 154–160Google Scholar
  7. Beruto M, Curir P & Debergh P (1999) Influence of agar on in vitro cultures: II. Biological performance of Ranunculus on media solidified with three different agar brands. In Vitro Cell. Dev. Biol. Plant 35: 94–101Google Scholar
  8. Beruto M, La Rosa C & Portogallo C (2001) Effects of agar impurities on in vitro propagation of Ranunculus asiaticus L. Acta Hort. 560: 399–402Google Scholar
  9. De Proft M, Maene L & Debergh P (1985) Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro. Physiol. Plant 65: 375–379Google Scholar
  10. Lercari G, Littardi M & Garibaldi E (1985) In vitro propagation of Ranunculus: experimental results. Riv. Ortoflorofrutt. It. 69: 321–326Google Scholar
  11. Maia E, Bettachini B, Beck D & Marais A (1973) Régénération de renoncules par culture d'apex in vitro. Ann. Phytopathol. 5: 125–129Google Scholar
  12. Meynet J (1985) Multiplication et sélection de la renoncule. Pépinièristes Horticultéurs Maraichers Revue Horticole, 254: 55–62Google Scholar
  13. Meynet J (1993) Ranunculus. In: De Hertogh A & Le Nard M (eds) The Physiology of Flower Bulbs (pp. 603–610). Elsevier Science Publishers, The NetherlandsGoogle Scholar
  14. Meynet J & Duclos A (1990a) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). I. Néoformation et multiplication végétative in vitro de plantes à partir de tron?on de thalamus. Agronomie 2: 157–162Google Scholar
  15. Meynet J & Duclos A (1990b) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). II. Production de plantes par culture d'anthères in vitro. Agronomie 10: 213–218Google Scholar
  16. Meynet J & Duclos A (1990c) Culture in vitro de la renoncule des fleuristes (Ranunculus asiaticus L.). III. Etude des plantes produites par embryogenèse somatique à partir des tissus superficiels de l'anthère. Agronomie 10: 285–290Google Scholar
  17. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497Google Scholar
  18. Pugliesi C, Rabaglio M, Cecconi F & Baroncelli S (1992) Plant regeneration from tissue cultures of Persian buttercup (Ranunculus asiaticus L.). Plant Cell Tiss. Org. Cult. 28: 125–128Google Scholar
  19. Statistisch Jaarboek (2001) Vereniging van Bloemenveilingen in Nederland (VBN), Leiden, Holland (p. 117)Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Istituto Regionale per la FloricolturaSanremo (IM)Italy
  2. 2.Department of Plant Sciences, Laboratory of Horticulture, Faculty of Agricultural and Applied Biological SciencesUniversity GentGentBelgium

Personalised recommendations