Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 77, Issue 1, pp 43–48 | Cite as

The Effect of Various Culture Media on the Formation of Embryo-like Structures in Cultures Derived from Explants Taken from Mature Larix decidua

  • J.M. Bonga
Article

Abstract

The effect of various collection dates and nine different culture media on the formation of ‘embryo-like structures’ (ELS) in cultures derived from explants taken from a 42-year-old Larix decidua tree was studied. The best medium was AFC, a medium low in NH4, NO3+, Mg2+ and SO42− but high in PO43− compared with the concentration of these elements in the other media. On AFC, ELS production reached a peak with collections made in late summer during the period when needle primordia are being initiated. For the other media, collection date had a lesser effect on ELS initiation.

conifers in vitro mature trees micropropagation somatic embryogenesis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Badaoui HEl, Morard P & Henry M (1996) Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture medium. Plant Cell Tiss. Org. Cult. 45: 153–158Google Scholar
  2. Bonga JM (1996) Frozen storage stimulates the formation of embryo-like structures and elongating shoots in explants from mature Larix decidua and L. × eurolepis trees. Plant Cell Tiss. Org. Cult. 46: 91–101CrossRefGoogle Scholar
  3. Bonga JM (1997) The effect of collection date and frozen storage on the formation of embryo-like structures and elongating shoots from explants from mature Larix decidua and L. × eurolepis. Plant Cell Tiss. Org. Cult. 51: 195–200CrossRefGoogle Scholar
  4. Danso KE & Ford-Lloyd BV (2002) Induction of high-frequency somatic embryos in cassava for cryopreservation. Plant Cell Rep. 21: 226–232Google Scholar
  5. Ewald D (1998) Advances in tissue culture of adult larch. In Vitro Cell. Dev. Biol. Plant 34: 325–330Google Scholar
  6. Klimaszewska K, Park YS, Overton C, MacEacheron I & Bonga JM (2001) Optimized somatic embryogenesis in Pinus strobus L. In Vitro Cell. Dev. Biol. Plant 37: 392–399Google Scholar
  7. Litvay JD, Verma DC & Johnson MA (1985) Influence of loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of wild carrot (Daucus carota L.). Plant Cell Rep. 4: 325–328CrossRefGoogle Scholar
  8. Monteiro ACB de A, Higashi EN, Goncalves AN & Rodriguez APM (2000) A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis Sims. F. Flavicarpa Deg.). In Vitro Cell. Dev. Biol. Plant 36: 527–531Google Scholar
  9. Park YS, Barrett JD & Bonga JM (1998) Application of somatic embryogenesis in high-value clonal forestry: Deployment, genetic control, and stability of cryopreserved clones. In Vitro Cell. Dev. Biol. Plant 34: 231–239Google Scholar
  10. Perrin M, Martin D, Joly D, Demangeat G, This P & Masson JE (2001) Medium-dependent response of grapevine somatic embryogenic cells. Plant Sci. 161: 107–116CrossRefGoogle Scholar
  11. Roustan JP, Latche A & Fallot J (1989) Stimulation of Daucus carota somatic embryogenesis by inhibitors of ethylene synthesis: cobalt and nickel. Plant Cell Rep. 8: 182–185CrossRefGoogle Scholar
  12. Scholten HJ & Pierik RLM(1998) Agar as a gelling agent: chemical and physical analysis. Plant Cell Rep. 17: 230–235CrossRefGoogle Scholar
  13. Smith DR (1997) The role of in vitro methods in pine plantation establishment: The lesson from New Zealand. Plant Tiss. Cult. Biotech. 3: 63–73Google Scholar
  14. Tremblay FM (1990) Somatic embryogenesis and plant regeneration from embryos isolated from stored seeds of Picea glauca. Can. J. Bot. 68: 236–242Google Scholar
  15. Westcott RJ (1994) Production of embryogenic callus from nonembryonic explants of Norway spruce Picea abies (L.) Karst. Plant Cell Rep. 14: 47–49CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Natural Resources CanadaCanadian Forest Service — Atlantic Forestry CentreFrederictonCanada

Personalised recommendations