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Russian Journal of Plant Physiology

, Volume 51, Issue 3, pp 294–301 | Cite as

Characteristics of Slow Induction Curve of Chlorophyll Fluorescence and CO2 Exchange for the Assessment of Plant Heat Tolerance at Various Levels of Light Intensity

  • E. N. Zavorueva
  • S. A. Ushakova
Article

Abstract

The heat tolerance of wheat (Triticum aestivum L.) and radish (Raphanus sativus L. var. minor) cenoses exposed to elevated and damaging air temperatures (35°C for 20 h, 45°C for 7 h) under photoculture conditions at various levels of photosynthetically active radiation (PAR) was assessed by measuring characteristics of the slow induction curve of chlorophyll fluorescence at 682 and 734 nm and the CO2 exchange rate. Irrespective of the illumination level, the exposure of the cenoses to 35°C did not induce irreversible changes in the plant photosynthetic apparatus. The lowest extent of damage to wheat and radish cenoses exposed to 45°C was observed at 150 W/m2 of PAR, whereas the highest damage of the plants was observed at an illumination level that was close to the compensation point of the cenose photosynthesis (50–70 W/m2 of PAR at air temperature of 24°C). Viability index proved to be the most sensitive characteristic, compared to other characteristics, which were determined by measuring the slow phase of fluorescence induction at 682 and 734 nm. In the cenoses studied, the pattern of changes in the viability index in response to a stress factor was close to the changes in the photosynthetic rate.

Triticum aestivum Raphanus sativus var. minor fluorescence heat tolerance pigments CO2 exchange 

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REFERENCES

  1. 1.
    Veselovskii, V.A., Veselova, T.V., and Chernavskii, D.S., Plant Stress: A Biophysical Approach, Fiziol. Rast. (Moscow), 1993, vol. 40, pp. 553-557 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  2. 2.
    Kosulina, L.G., Lutsenko, E.K., and Akimova, V.A., Physiology of Plant Resistance to Unfavorable Environment, Rostov-on-Don: Rostov. Gos. Univ., 1993.Google Scholar
  3. 3.
    Bukhov, N.G., Boucher, N., and Carpentier, R., Aftereffect of Short-Term Heat Shock on Photosynthetic Reactions in Barley Leaves, Fiziol. Rast. (Moscow), 1997, vol. 44, pp. 605-612 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  4. 4.
    Sharkova, V.E. and Bubolo, L.S., Effect of Heat Stress on the Arrangement of Thylakoid Membranes in the Chloroplasts of Mature Wheat Leaves, Fiziol. Rast. (Moscow), 1996, vol. 44, pp. 409-417 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  5. 5.
    Chauhan, Y.S. and Senboku, T., Thermostabilities of Cell Membrane and Photosynthesis in Cabbage Cultivars Differing in Heat Tolerance, J. Plant Physiol., 1996, vol. 149, pp. 727-734.Google Scholar
  6. 6.
    Yusufov, A.G. and Alieva, Z.M., Viability of Plants and Isolated Organs under Salinity Conditions, Fiziol. Rast. (Moscow), 2002, vol. 49, pp. 553-557 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  7. 7.
    Lichtenthaler, H.K., The Role of Chlorophyll Fluorescence in the Detection Stress Condition in Plants, CRC Crit. Rev. Anal. Chem., 1988, vol. 19, pp. 29-85.Google Scholar
  8. 8.
    Lichtenthaler, H.K. and Burhart, S., Photosynthesis and High Light Stress, Bulg. J. Plant Physiol., 1999, vol. 25, pp. 3-16.Google Scholar
  9. 9.
    Lisovskii, G.M. and Shilenko, M.P., Selection of Structure and Conditions for the Functioning of the Branch “Higher Plants,” Zamknutaya sistema: chelovek-vysshie rasteniya (Closed System: Man-Higher Plants), Lisovskii, G.M., Ed., Novosibirsk: Nauka, 1979, pp. 38-52.Google Scholar
  10. 10.
    Ushakova, S.A. and Tikhomirov, A.A., Tolerance of LSS Plant Component to Elevated Temperature, Acta Astronautica, 2002, vol. 12, pp. 759-764.Google Scholar
  11. 11.
    Wintermans, I.F. and de Mots, A., Spectrophotometric Characteristics of Chlorophyll a and b and Their Pheophytins in Ethanol, Biochim. Biophys. Acta, 1965, vol. 109, pp. 448-453.Google Scholar
  12. 12.
    Zavorueva, E.N., Ushakova, S.A., Volkova, E.K., Tikhomirov, A.A., Mogil'naya, O.A., and Medvedeva, S.M., Fine Chloroplast Structure in Cucumber and Pea Leaves Developed under Red Light, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 843-851 (Russ. J. Plant Physiol., Engl. Transl.).Google Scholar
  13. 13.
    Zavoruev, V.V., Zavorueva, E.N., and Shelegov, A.V., Fluorescence Induced by Light of 380-540 nm in Leaves of Cucumber Depending on the Vegetation Time and Irradiation Regime, Biofizika, 2000, vol. 45, pp. 704-711.Google Scholar
  14. 14.
    Veselovskii, V.A. and Veselova, T.V., Lyuminestsentsiya rastenii (Luminescence of Plants), Moscow: Nauka, 1990.Google Scholar
  15. 15.
    Karavaev, V.A., Shagurina, T.L., Kukushkin, A.K., and Solntsev, M.K., Correlation between Changes of Fast and Slow Fluorescence Induction in Leaves of Bean Plants in the Presence of Herbicides and Antioxidants, Fiziol. Rast. (Moscow), 1987, vol. 34, pp. 60-66 (Sov. Plant Physiol., Engl. Transl.).Google Scholar
  16. 16.
    Tuba, Z., Lichtenthaler, H.K., Csintalan, Z., Nagy, Z., and Szente, K., Reconstitution of Chlorophylls and Photosynthetic CO2 Assimilation in the Disiccated Poikilochlorophyllous Plant Xerophytas cabida upon Rehydration, Planta, 1994, vol. 192, pp. 414-420.Google Scholar
  17. 17.
    Weston, E., Tyorogood, K., Vinti, G., and Lopez-Juez, E., Quantity Controls Leaf Cell and Chloroplast Development in Arabidopsis thaliana Wild Type and Blue Light Perception in Mutant, Planta, 2000, vol. 211, pp. 807-815.Google Scholar
  18. 18.
    Lenets, A.A., Transgenic Tobacco Plants as a Model for Research of Structural and Functional Organization of the Photosynthetic Apparatus under Stress Adaptation, Mater. Vseros. s”ezda fotobiologov, Pushchino, 8–12 iyunya, 1998 (Proc. All-Russia Congr. of Photobiologists), Pushchino, 1998, pp. 72-74.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • E. N. Zavorueva
    • 1
  • S. A. Ushakova
    • 2
  1. 1.Krasnoyarsk State Architectural and Building AcademyKrasnoyarskRussia
  2. 2.Institute of Biophysics, Siberian DivisionRussian Academy of SciencesAkademgorodok, KrasnoyarskRussia

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