Russian Journal of Genetics

, Volume 40, Issue 1, pp 1–9 | Cite as

Molecular Genetic Characteristics of Medieval Populations of the Russian North

  • E. E. Kulikov
  • A. P. Buzhilova
  • A. B. Poltaraus
Article

Abstract

Forty-seven individual mitochondrial DNA (mtDNA) samples isolated from bones samples found in the Nefedyevo, Minino, and Shuygino gravesites have been analyzed to perform molecular genetic study of the medieval (12th to 13th centuries AD) human population from the vicinity of Lake Beloe (Vologda oblast, northern Russia). The mitotypic structure of the population has been determined on the basis of sequencing the mtDNA hypervariable-region segment I (HVSI; positions 15 989–16 410). Three mitotypes characterizing the population studied have been found in the 47 representatives of the medieval population: mitotype 1 corresponding to the Cambridge reference sequence, mitotype 2 (transition G–A at position 16 129), and mitotype 3 (transitions G–A and C–T at loci 16  129 and 16  223, respectively). Mitotypes 1, 2, and 3 have been found in 91.6, 4.2, and 4.2% of the individual samples studied. This high frequency of the Cambridge mitotype is considerably higher than its mean frequencies in European populations. The frequencies of other mitotypes found correspond to their mean European values. The absence of a Mongoloid component has been demonstrated for the female lineage of the population. Comparison of the molecular genetic characteristics of contemporary European ethnic groups and the population studied has demonstrated that it may be assigned to the European population group. The high homogeneity of the mitochondrial pool suggests a strong founder effect, which agrees with the view of archeologists and anthropologists that the first migrant settlers were very few.

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REFERENCES

  1. 1.
    Alekseeva, T.I., Etnogenez vostochnykh slavyan po dannym antropologii (Ethnogenesis of Eastern Slavs by Anthropological Data), Moscow: Nauka, 1973.Google Scholar
  2. 2.
    Sedov, V.V., Anthropological Types of the Population of Northwestern Regions of Velikii Novgorod, Kratkie soobshcheniya Instituta etnografii (Short Communications of the Institute of Ethnography), Moscow: Mosk. Gos. Univ., 1952, no. 15.Google Scholar
  3. 3.
    Sankina, S.L., The Anthropological Composition and Origin of the Population of the Novgorod Region, Cand. Sci. (Hist.) Dissertation, St. Petersburg, 1995.Google Scholar
  4. 4.
    Alekseeva, T.I., Ethnogenesis and Ethnic History of Eastern Slavs, in Vostochnye slavyane. Antropologiya i etnicheskaya istoriya (Eastern Slavs: Anthropology and Ethnic History), Moscow: Nauchnyi Mir, 1999.Google Scholar
  5. 5.
    Makarov, N.A., Naselenie Russkogo Severa v XI–XIII vv. Po materialam mogil'nikov Vostochnogo Prionezh'ya (The Population of the Russian North in the 11th–13th Centuries: Evidence from Tombs of the East Onega Region), Moscow: Nauchnyi Mir, 1990.Google Scholar
  6. 6.
    Alekseeva, T.I., Makarov, N.A., Balueva, T.S., et al., Early Steps in Colonization of the Russian North: History, Anthropology, Ecology, in Ekologicheskie problemy v issledovaniyakh srednevekovogo naseleniya Vostochnoi Evropy (Ecological Problems in Studies of the medieval Population of Eastern Europe), Moscow: Nauchnyi Mir, 1993, pp. 3-78.Google Scholar
  7. 7.
    Limborskaya, S.A., Khusnutdinova, E.K., and Balanovskaya, E.V., Etnogenomika i genogeografiya narodov Vostochnoi Evropy (Ethnogenomics and Gene Geography of East European Ethnic Groups), Moscow: Nauka, 2002.Google Scholar
  8. 8.
    Hoss, M. and Paabo, S., DNA Extraction from Pleistocene Bones by a Silica-Based Purification Method, Nucleic Acids Res., 1993, vol. 21,no. 16, pp. 3913-3914.Google Scholar
  9. 9.
    Gill, P., Ivanov, P.L., Kimpton, C., et al., Identification of the Remains of the Romanov Family by DNA Analysis, Nat. Genet., 1994, vol. 6,no. 2, pp. 130-135.Google Scholar
  10. 10.
    Krings, M., Stone, A., Schmitz, R.W., et al., Neanderthal DNA Sequences and the Origin of Modern Humans, Cell (Cambridge, Mass.), 1997, vol. 90,no. 1, pp. 19-30.Google Scholar
  11. 11.
    Makarov, N.A., Kolonizatsiya severnykh okrain Drevnei Rusi v XI-XIII vv. Po materialam arkheologicheskikh pamyatnikov na volokakh Belozer'ya i Poonezh'ya (Colonization of the Northern Remote Regions of Ancient Rus' in the 11th-13th Centuries: Evidence from Relics Found along the Drag Pathways of the Beloe and Onezhskoe Lakes Regions), Moscow, 1997.Google Scholar
  12. 12.
    Makarov, N.A. and Zaitseva, I.E., New Studies of Medieval Tombs in the Russian North: The Minino II Tomb at the Kubenskoe Lake, Ross. Arkheol., 1999, vol. 4, pp. 163-179.Google Scholar
  13. 13.
    Kaestle, F.A. and Horsburgh, A.K., Ancient DNA in Anthropology: Methods, Applications, and Ethics, Yearbook Phys. Anthropol., 2002, vol. 45, pp. 92-130.Google Scholar
  14. 14.
    Boom, R., Sol, C.J., Salimans, M.M., et al., Rapid and Simple Method for Purification of Nucleic Acids, J. Clin. Microbiol., 1990, vol. 28,no. 3, pp. 495-503.Google Scholar
  15. 15.
    Anderson, S., Bankier, A.T., Barrell, B.G., et al., Sequence and Organization of the Human Mitochondrial Genome, Nature, 1981, vol. 290,no. 5806, pp. 457-465.Google Scholar
  16. 16.
    Ovchinnikov, I.V., Ovchinnikova, O.I., Druzina, E.B., et al., Molecular Genetic Sex Determination of medieval Human Remains from North Russia: Comparison with Archaeological and Anthropological Criteria, Anthropol. Anzeiger, 1998, vol. 56,no. 1, pp. 7-15.Google Scholar
  17. 17.
    Richards, M., Macaulay, V., Hickey, E., et al., Tracing European Founder Lineages in the Near Eastern mtDNA Pool, Am. J. Hum. Genet., 2000, vol. 67,no. 5, pp. 1251-1276.Google Scholar
  18. 18.
    Macaulay, V., Richards, M., Hickey, E., et al., The Emerging Tree of West Eurasian mtDNAs: A Synthesis of Control-Region Sequences and RFLPs, Am. J. Hum. Genet., 1999, vol. 64, pp. 232-249.Google Scholar
  19. 19.
    Simoni, L., Calafell, F., Pettener, D., et al., Geographic Patterns of mtDNA Diversity in Europe, Am. J. Hum. Genet., 2000, vol. 66,no. 1, pp. 262-278.Google Scholar
  20. 20.
    Sajantila, A., Lahermo, P., Anttinen, T., et al., Genes and Languages in Europe: An Analysis of Mitochondrial Lineages, Genome Res., 1995, vol. 5,no. 1, pp. 42-52.Google Scholar
  21. 21.
    Malyarchuk, B.A. and Derenko, M.V., Human Mitochondrial DNA Variation: A Distribution of Hot Points in Hypervariable Segment I of the Major Noncoding Region, Genetika (Moscow), 2001, vol. 37,no. 7, pp. 991-1001.Google Scholar
  22. 22.
    Torroni, A., Chen, Y.S., Semino, O., et al., mtDNA and Y-Chromosome Polymorphisms in Four Native American Populations from Southern Mexico, Am. J. Hum. Genet., 1994, vol. 54,no. 2, pp. 303-318.Google Scholar
  23. 23.
    Malyarchuk, B.A., Differentiation of Slavs and Their Genetic Position among Eurasian Ethnic Groups as Inferred from the Mitochondrial DNA Variation, Genetika (Moscow), 2001, vol. 37,no. 12, pp. 1705-1712.Google Scholar
  24. 24.
    Orekhov, V., Poltaraus, A., Zhivotovsky, L.A., et al., Mitochondrial DNA Sequence Diversity in Russians, FEBS Lett., 1999, vol. 445,no. 1, pp. 197-201.Google Scholar
  25. 25.
    Buzhilova, A.P., Biological and Social Adaptation of the Population of the Russian North (Evidence from Anthropological Materials of the Beloe and Onezhskoe Lake Regions), Srednevekovoe rasselenie na Belom ozere (Medieval Settlements at the Beloe Lake), Makarov, N.A., Zakharov, S.D., and Buzhilova A.P., Eds., Moscow, 2001, pp. 227-276.Google Scholar
  26. 26.
    Sajantila, A., Salem, A.H., Savolainen, P., et al., Paternal and Maternal DNA Lineages Reveal a Bottleneck in the Founding of the Finnish Population, Proc. Natl. Acad. Sci. USA, 1996, vol. 93,no. 21, pp. 12 035-12 039.Google Scholar
  27. 27.
    Thomas, M.G., Weale, M.E., Jones, A.L., et al., Founding Mothers of Jewish Communities: Geographically Separated Jewish Groups Were Independently Founded by Very Few Female Ancestors, Am. J. Hum. Genet., 2002, vol. 70,no. 6, pp. 1411-1420.Google Scholar
  28. 28.
    De La Chapelle, A., Disease Gene Mapping in Isolated Human Populations: The Example of Finland, J. Med. Genet., 1993, vol. 67, pp. 857-865.Google Scholar
  29. 29.
    Gonzalez-Oliver, A., Marquez-Morfin, L., Jimenez, J.C., and Torre-Blanco, A., Founding Amerindian Mitochondrial DNA Lineages in Ancient Maya from Xcaret, Quintana Roo, Am. J. Phys. Anthropol., 2001, vol. 116,no. 3, pp. 230-235.Google Scholar
  30. 30.
    Merriwether, D.A., Huston, S., Iyengar, S., et al., Mitochondrial versus Nuclear Admixture Estimates Demonstrate a Past History of Directional Mating, Am. J. Phys. Anthropol., 1997, vol. 102,no. 2, pp. 153-159.Google Scholar
  31. 31.
    Oota, H., Kurosaki, K., Pookajorn, S., et al., Genetic Study of the Paleolithic and Neolithic Southeast Asians, Hum. Biol., 2001, vol. 73,no. 2, pp. 225-231.Google Scholar
  32. 32.
    Naumova, O.Yu., Rychkov, S.Yu., Bazaliiskii, V.I., et al., Molecular Genetic Characterization of the Neolithic Population of the Baikal Region: RFLP Analysis of Ancient mtDNA from Fossil Bones of the Ust'-Ida Tomb, Genetika (Moscow), 1997, vol. 33,no. 10, pp. 1418-1425.Google Scholar

Copyright information

© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • E. E. Kulikov
    • 1
  • A. P. Buzhilova
    • 2
  • A. B. Poltaraus
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Russian Academy of SciencesMoscowRussia

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