Russian Journal of Genetics

, Volume 39, Issue 11, pp 1292–1305 | Cite as

Molecular Evolution of Satellite DNA CLsat in Lizards from the Genus Darevskia (Sauria: Lacertidae): Correlation with Species Diversity

  • D. G. Ciobanu
  • V. V. Grechko
  • I. S. Darevsky
Article

Abstract

The structure and evolution of a satellite DNA family was examined in lizards from the genus Darevskia(family Lacertidae). Comparison of tandem units of repeated DNA (satDNA), CLsat, in all species from the genus Darevskiahas shown that their variability is largely explained by single-nucleotide substitutions, which form about 50 diagnostic positions underlying classification of the family into three subfamilies. Maximum differences between the subfamilies reached 25%. At this level of tandem unit divergence in the subfamilies, no cross-hybridization between them was observed (at 65°C). The individual variability within one subfamily within the species was on average 5% while the variability between species consensuses within a subfamily was 10%. The presence of highly conserved regions in all monomers and some features of their organization show that satellites of all Darevskia species belong to one satDNA family. The organization of unit sequences of satellites CLsat and Agi160 also detected by us in another lizard genus, Lacerta s. str. was compared. Similarity that was found between these satellites suggests their relatedness and common origin. A possible pathway of evolution of these two satDNA families is proposed. The distribution and content of CLsat repeat subfamilies in all species of the genus was examined by Southern hybridization. Seven species had mainly CLsatI (83 to 96%); three species, approximately equal amounts of CLsatI and CLsatIII (the admixture of CLsatII was 2–5%); and five species, a combination of all three subfamilies in highly varying proportions. Based on these results as well as on zoogeographic views on the taxonomy and phylogeny of theDarevskia species, hypotheses on the evolution of molecular-genetic relationships within this genus are advanced.

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Copyright information

© MAIK “Nauka/Interperiodica” 2003

Authors and Affiliations

  • D. G. Ciobanu
    • 1
  • V. V. Grechko
    • 1
  • I. S. Darevsky
    • 2
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Zoological InstituteRussian Academy of SciencesSt. PetersburgRussia

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