Plant and Soil

, Volume 264, Issue 1–2, pp 287–297 | Cite as

Accumulation and within-seed distribution of iron in common bean and soybean

  • J.T. Moraghan


The influence of times of applying FeEDDHA on seed yield and Fe accumulation by four common bean (Phaseolus vulgaris L.) and two soybean (Glycine max L.) genotypes grown on a calcareous soil was studied under greenhouse conditions. The soybean genotypes, unlike the common bean genotypes, developed Fe-deficiency chlorosis and responded to application of the chelate. A preplant application of FeEDDHA was more efficacious than a flowering application in increasing seed yield of soybean. In contrast, the flowering application was much more effective than the preplant application for increasing seed Fe concentration [Fe] of both species. Percentage of seed Fe located in the seed coat of the common bean genotypes ranged from approximately 5 to 40% and was little affected by FeEDDHA. This within-seed distribution of Fe was correlated with methanol-extractable seed-coat pigments absorbing at 500 nm, presumably anthocyanins, but not with condensed tannins (proanthocyanidins). The soybean genotypes did not accumulate anthocyanins or tannins in the seed coat. Seed of Fe-deficient soybean plants without FeEDDHA had appreciably lower [Fe] and had a lower percentage of seed Fe in the seed coat than treated plants. Within-seed distribution of Fe should be considered in plant breeding because of concerns about both human nutrition and early seedling growth.

Abbreviations: DTPA – diethylenetrinitrilopentaacetic acid; EDDHA – ethylenediamine di(o-hydroxyphenylacetic acid) acid; SPAD – single photon avalanche diode

anthocyanins Glycine max iron efficiency Phaseolus vulgaris seed Fe tannins 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • J.T. Moraghan
    • 1
  1. 1.Department of Soil ScienceNorth Dakota State UniversityFargoUSA

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