Plant and Soil

, Volume 260, Issue 1–2, pp 183–196 | Cite as

Elevated [CO2] effects on herbage production and soil carbon and nitrogen pools and mineralization in a species-rich, grazed pasture on a seasonally dry sand

  • D.J. Ross
  • P.C.D. Newton
  • K.R. Tate


Rising concentrations of atmospheric [CO2] in a multi-species ecosystem can influence species composition and increase plant productivity, but have a less predictable effect on soil C storage and nutrient availability. Using a free-air [CO2]-enriched (FACE) system and seasonal sampling over a 5-year period, we examined the influence of elevated atmospheric [CO2] (475 μL L−1) on soil C and N pools and mineralization in a fertilized (P, K, S), sheep-grazed pasture of mixed grass, clover, and forb species on a seasonally dry sand (Mollic Psammaquent). Annual yields of herbage dry matter ranged from about 300 to 1600 g m−2. Total yields did not increase significantly under elevated [CO2], but the proportions of clovers and forbs increased markedly. Most properties in 0–50 mm-depth soil differed significantly (P<0.05) with year of sampling, but [CO2]-treatment effects were non-significant (P>0.10) for moisture, pH, total C and N, extractable C and organic N, microbial C, and mineral-N. However, microbial N, CO2-C production (0–14 days) in field-moist soil, and net mineral-N production (14–56 days) in soil at 60% of water-holding capacity were significantly higher (per unit weight of soil) in the elevated-[CO2] treatment (P=0.071, 0.063, 0.003, respectively); the degree of these treatment differences was roughly similar when values were also expressed on a total C or N basis. Relationships with soil moisture were mainly non-significant for microbial C and N, but mainly significant (P<0.05) for net mineral-N production in field-moist soil, and highly significant (P<0.001) for CO2-C production. Overall, the data tend to suggest greater soil metabolic activity, but little if any change in soil C pools, after 5 years' exposure of the pasture to elevated [CO2]. They do, however, suggest increased availability of N, probably because of increased inputs from N-fixing clovers.

dissolved organic carbon dissolved organic nitrogen heterotrophic respiration nitrification nitrogen mineralization plant production 


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  1. Allard V, Newton P C D, Lieffering M, Soussana J-P, Carran R A and Matthew C 2004 Increased quantity and quality of coarse soil organic matter fraction at elevated CO2 in a grazed grassland are a consequence of enhanced root growth and turnover, Plant Soil (In press).Google Scholar
  2. Anderson J P E and Domsch K H 1980 Quantities of plant nutrients in the microbial biomass of selected soils. Soil Sci. 130, 211–216.Google Scholar
  3. Bardgett R D, Levell R H, Hobbs P J and Jarvis S C 1999 Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands. Soil Biol. Biochem. 31, 1021–1030.Google Scholar
  4. Blakemore L C, Searle P L and Daly B K 1987 Methods for chemical analysis of soils. N. Z. Soil Bur. Sci. Rep. 80.Google Scholar
  5. Burke I C, Lauenroth W K and Milchunas D G 1997 Biogeochemistry of managed grasslands in central North America. In Soil Organic Matter in Temperate Agroecosystems. Eds. E A Paul, E T Elliott, K Paustian, C V Cole. pp. 85–102. CRC Press Inc., New York.Google Scholar
  6. Butler G W, Greenwood R M and Soper K 1959 Effects of shading and defoliation on the turnover of root and nodule tissue of plants of Trifolium repens, Trifolium pratense, and Lotus uliginosus. N. Z. J. Agric. Res. 2, 415–426.Google Scholar
  7. Carran R A 1979 Soil nitrogen and pasture management. Proc. N. Z. Grassl. Assoc. 1978, 44–50.Google Scholar
  8. Corre M D, Schnabel R R and Stout W L 2002 Spatial and seasonal variation of gross nitrogen transformations and microbial biomass in a Northeastern US grassland. Soil Biol. Biochem. 34, 445–457.Google Scholar
  9. Cowie J D and Hall A D 1965 Soils and agriculture of Flock House, Bulls, Manawatu, N. Z. N. Z. Soil Bur Rep.Google Scholar
  10. Daepp M, Suter D, Almeida J P F, Isopp H, Hartwig U A, Frehner M, Blum H, Nösberger J and Lüscher A 2000 Yield response of Lolium perenne swards to free-air CO2 enrichment increased over six years in a high N input system on fertile soil. Glob. Change Biol. 6, 805–816.Google Scholar
  11. Edwards G R, Clark H and Newton P C D 2001a The effects of elevated CO2 on seed production and seedling recruitment in a sheep-grazed pasture. Oecologia 127, 383–394.Google Scholar
  12. Edwards G R, Newton P C D, Tilbrook J C and Clark H 2001b Seedling performance of pasture species under elevated CO2. New Phytol. 150, 359–369.Google Scholar
  13. Genstat (2002) Genstat 6, Release 1.0.20. Lawes Agricultural Trust, IACR, Rothamsted.Google Scholar
  14. Gill R A, Polley H W, Johnson H B, Anderson L J, Maherali H and Jackson R B 2002 Nonlinear grassland responses to past and future atmospheric CO2. Nature 417, 279–282.Google Scholar
  15. Guitian R and Bardgett R D 2000 Plant and soil microbial responses to defoliation in temperate semi-natural grassland. Plant Soil 220, 271–277.Google Scholar
  16. Hardacre A K, Laing W A and Christeler J T 1986 The response of simulated swards of perennial ryegrass and white clover to enriched atmospheric CO2: interaction with nitrogen and photosynthetic photon flux density. N. Z. J. Agric. Res. 29, 567–573.Google Scholar
  17. Hungate B A, Chapin F S III, Zhang H, Holland E A and Field C B 1997a Stimulation of grassland nitrogen cycling under carbon dioxide enrichment. Oecologia 109, 149–153.Google Scholar
  18. Hungate B A, Holland E A, Jackson R B, Chapin F S III, Mooney H A and Field C B 1997b The fate of carbon in grasslands under carbon dioxide enrichment. Nature 388, 576–579.Google Scholar
  19. Hungate B A, Jaeger C H III, Gemara G, Chapin F S III and Field C B 2000 Soil microbiota in two annual grasslands: responses to elevated atmospheric CO2. Oecologia 124, 589–598.Google Scholar
  20. IGBP Terrestrial Carbon Working Group 1998 The terrestrial carbon cycle: implications for the Kyoto protocol. Science 280, 1393–1394.Google Scholar
  21. IPCC 2001 Climate Change 2001: Mitigation. Cambridge University Press, Cambridge.Google Scholar
  22. Jastrow J D, Miller R M and Owensby C E 2000 Long-term effects of elevated atmospheric CO2 on below-ground biomass and transformations to soil organic matter in grassland. Plant Soil 224, 85–97.Google Scholar
  23. Jenkinson D S 1988 Determination of microbial biomass carbon and nitrogen in soil. In Advances in Nitrogen Cycling in Agricultural Ecosystems. Ed. J R Wilson. pp. 368–386. CAB International, Wallingford.Google Scholar
  24. Jones T H, Thompson L J, Lawton J H, Bezemer T M, Bardgett R D, Blackburn T M, Bruce K D, Cannon P F, Hall G S, Hartley S E, Howson G, Jones C G, Kampichler C, Kandeler E and Ritchie D A 1998 Impacts of rising atmospheric carbon dioxide on model terrestrial ecosystems. Science 280, 441–443.Google Scholar
  25. Leadley P W, Niklaus P A, Stocker R and Körner C 1999 A field study of the effects of elevated CO2 on plant biomass and community structure in a calcareous grassland. Oecologia 118, 39–49.Google Scholar
  26. Ledgard S F and Steele K W 1992 Biological nitrogen fixation in mixed legume/grass pastures. Plant Soil 141, 137–153.Google Scholar
  27. Loiseau P and Soussana J F 2000 Effects of elevated CO2, temperature and N fertilization on nitrogen fluxes in a temperate grassland ecosystem. Glob. Change Biol. 6, 953–965.Google Scholar
  28. Lüscher A, Hartwig U A, Suter D and Nösberger J 2000 Direct evidence that symbiotic N2 fixation in fertile grassland is an important trait for a strong response of plants to elevated atmospheric CO2. Glob. Change Biol. 6, 655–662.Google Scholar
  29. Marissink M, Pettersson R and Sindhøj E 2002 Above-ground plant production under elevated carbon dioxide in a Swedish semi-natural grassland. Agric, Ecosyst. Environ. 93, 107–120.Google Scholar
  30. Melillo J M, McGuire A D, Kicklighter D W, Moore B III, Verosmarty C J and Schloss A L 1993 Global climate change and terrestrial net primary production. Nature 363, 234–239.Google Scholar
  31. Newton P C D, Clark H, Bell C C, Glasgow E M and Campbell B D 1994 Effects of elevated CO2 and simulated seasonal changes in temperature on the species composition and growth rate of pasture turves. Ann. Bot. 73, 53–59.Google Scholar
  32. Newton P C D, Clark H and Edwards G R 2001 The effect of climate change on grazed grasslands. In Structure and Function of Agroecosystem Design and Management. Eds. M Shiyomi, H Koizumi. pp. 297–311. CRC Press, Boca Raton, Florida.Google Scholar
  33. Niklaus P A 1998 Effects of elevated atmospheric CO2 on soil microbiota in calcareous grassland. Glob. Change Biol. 4, 451–458.Google Scholar
  34. Niklaus P A, Leadley P W, Stöcklin J and Körner C 1998a Nutrient relations in calcareous grassland under elevated CO2. Oecologia 116, 67–75.Google Scholar
  35. Niklaus P A, Spinnler D and Körner C 1998b Soil moisture dynamics of calcareous grassland under elevated CO2. Oecologia 117, 201–208.Google Scholar
  36. Niklaus P A, Kandeler E, Leadley P W, Schmid B, Tscherko D and Körner C 2001a A link between plant diversity, elevated CO2 and soil nitrate. Oecologia 127, 540–548.Google Scholar
  37. Niklaus P A, Wohlfender M, Siegwolf R and Körner C 2001b Effects of six years atmospheric CO2 enrichment on plant, soil, and soil microbial C of a calcareous grassland. Plant Soil 233, 189–202.Google Scholar
  38. Niklaus P A, Alphei J, Ebersberger D, Kampichler C, Kandeler E and Tscherko D 2003 Six years of in situ CO2 enrichment evoke changes in soil structure and soil biota of nutrient-poor grassland. Glob. Change. Biol. 9, 585–600.Google Scholar
  39. Owensby C E, Coyne P I, Ham J M, Auen L Mand Knapp A K 1993 Biomass production in a tallgrass prairie ecosystem exposed to ambient and elevated CO2. Ecol. Appl. 3, 644–653.Google Scholar
  40. Owensby C E, Ham J M, Knapp A K and Auen L M 1999 Biomass production and species composition change in a tallgrass prairie ecosystem after long-term exposure to elevated atmospheric CO2. Glob. Change Biol. 5, 497–506.Google Scholar
  41. Parton W J, Schimel D S, Cole C V and Ojima D S 1987 Analysis of factors controlling soil organic matter levels in Great Plains grasslands. Soil Sci. Soc. Am. J. 51, 1173–1179.Google Scholar
  42. Paterson E, Rattray E A S and Killham K 1996 Effect of elevated atmospheric CO2 concentration on C-partitioning and rhizosphere C-flow for three plant species. Soil Biol. Biochem. 28, 195–201.Google Scholar
  43. Patra D D, Brookes P C, Coleman K and Jenkinson D S 1990 Seasonal changes of soil microbial biomass in an arable and a grassland soil which have been under uniform management for many years. Soil Biol. Biochem. 22, 739–742.Google Scholar
  44. Ross D J and Tate K R 1993 Microbial C and N in litter and soil of a southern beech (Nothofagus) forest: comparison of measurement procedures. Soil Biol. Biochem. 25, 467–475.Google Scholar
  45. Ross D J, Saggar S, Tate K R, Feltham C W and Newton P C D 1996 Elevated CO2 effects on carbon and nitrogen cycling in grass/clover turves of a Psammaquent soil. Plant Soil 182, 185–198.Google Scholar
  46. Ross D J, Speir T W, Kettles H A and Mackay A D 1995 Soil microbial biomass, C and N mineralization and enzyme activities in a hill pasture: influence of season and slow-release P and S fertilizer. Soil Biol. Biochem. 27, 1431–1443.Google Scholar
  47. Ross D J, Tate K R, Newton P C D, Wilde R H and Clark H 2000 Carbon and nitrogen pools and mineralization in a grassland gley soil under elevated carbon dioxide at a natural CO2 spring. Glob. Change Biol. 6, 779–790.Google Scholar
  48. Ruess R W and McNaughton S J 1987 Grazing and the dynamics of nutrient and energy regulated microbial processes in the Serengeti grasslands. Oikos 49, 101–110.Google Scholar
  49. Scott N A, Parfitt R L, Ross D J and Salt G J 1998 Carbon and nitrogen transformations in New Zealand plantation forest soils from sites with different N status. Can. J. For. Res. 28, 967–976.Google Scholar
  50. Six J, Carpentier A, van Kessel C, Merckx R, Harris D, Horwath WR and Leischer A 2001 Impact of elevated CO2 on soil organic matter dynamics as related to changes in aggregate turnover and residue quality. Plant Soil 234, 27–36.Google Scholar
  51. Soussana J F and Hartwig U A 1996 The effects of elevated CO2 on symbiotic N2 fixation: a link between the carbon and nitrogen cycles in grassland ecosystems. Plant Soil 187, 321–332.Google Scholar
  52. Stöcklin J and Körner Ch. 1999 Interactive effects of elevated CO2, P availability and legume presence on calcareous grassland: results of a glasshouse experiment. Funct. Ecol. 13, 200–209.Google Scholar
  53. SYSTAT 1996 SYSTAT 6.0 for Windows: Statistics. SPSS Inc., Chicago, Il.Google Scholar
  54. Teyssonneyre F, Picon-Cochard C, Falcimagne R and Sousanna J-F 2002 Effects of elevated CO2 and cutting frequency on plant. community structure in a temperate grassland. Glob. Change Biol. 8, 1034–1046.Google Scholar
  55. Van Ginkel J H, Gorissen A and van Veen J A 1997 Carbon and nitrogen allocation in Lolium perenne in response to elevated atmospheric CO2 with emphasis on soil carbon dynamics. Plant Soil 188, 299–308.Google Scholar
  56. Van Groenigen K-J, Harris D, Horwath W R, Hartwig U E and van Kessel C 2002 Linking sequestration of 13C and 15N in aggregates in a pasture soil following 8 years of elevated CO2. Glob. Change Biol. 8, 1094–1108.Google Scholar
  57. Van Kessel C, Horwath W R, Hartwig U, Harris D, Lüscher A 2000 Net soil carbon input under ambient and elevated CO2 concentrations: isotopic evidence after 4 years. Glob. Change Biol. 6, 435–444.Google Scholar
  58. Van Veen J A, Liljeroth E, Lekkerkerk L J A and van de Geijn S C 1991 Carbon fluxes in plant-soil systems at elevated atmospheric CO2 levels. Ecol. Applic. 1, 175–181.Google Scholar
  59. Von Caemmerer S, Ghannoum O, Conroy J P, Clark H and Newton P C D 2001 Photosynthetic responses of temperate species to free air CO2 enrichment (FACE) in a grazed New Zealand pasture. Aust. J. Plant Physiol. 28, 439–450.Google Scholar
  60. Williams M A, Rice C W and Owensby C E 2000 Carbon dynamics and microbial activity in tallgrass prairie exposed to elevated CO2 for 8 years. Plant Soil 227, 127–137.Google Scholar
  61. Williams M A, Rice C and Owensby C E 2001 Nitrogen competition in a tallgrass prairie ecosystem exposed to elevated carbon dioxide. Soil Sci. Soc. Am. J. 65, 340–346.Google Scholar
  62. Yeates G W, Newton P C D and Ross D J 2003 Significant changes in soil microfauna in grazed pasture under elevated carbon dioxide. Biol. Fert. Soils. 38, 319–326.Google Scholar
  63. Zak D R, Pregitzer K S, King J S and Holmes W E 2000 Elevated atmospheric CO2, fine roots and the response of soil microrganisms: a review and hypothesis [Review]. New Phytol. 147, 201–222.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • D.J. Ross
    • 1
  • P.C.D. Newton
    • 2
  • K.R. Tate
    • 1
  1. 1.Landcare ResearchPalmerston NorthNew Zealand
  2. 2.AgResearch GrasslandsPalmerston NorthNew Zealand

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