Plant Molecular Biology

, Volume 54, Issue 5, pp 743–753

Overexpression of Multiple Dehydrin Genes Enhances Tolerance to Freezing Stress in Arabidopsis

  • Tuula Puhakainen
  • Michael W. Hess
  • Pirjo Mäkelä
  • Jan Svensson
  • Pekka Heino
  • E. Tapio Palva
Article

Abstract

To elucidate the contribution of dehydrins (DHNs) to freezing stress tolerance in Arabidopsis, transgenic plants overexpressing multiple DHN genes were generated. Chimeric double constructs for expression of RAB18andCOR47(pTP9) or LTI29 and LTI30(pTP10) were made by fusing the coding sequences of the respective DHN genes to the cauliflower mosaic virus 35S promoter. Overexpression of the chimeric genes in Arabidopsis resulted in accumulation of the corresponding dehydrins to levels similar or higher than in cold-acclimated wild-type plants. Transgenic plants exhibited lower LT50 values and improved survival when exposed to freezing stress compared to the control plants. Post-embedding immuno electron microscopy of high-pressure frozen, freeze-substituted samples revealed partial intracellular translocation from cytosol to the vicinity of the membranes of the acidic dehydrin LTI29 during cold acclimation in transgenic plants. This study provides evidence that dehydrins contribute to freezing stress tolerance in plants and suggests that this could be partly due to their protective effect on membranes.

Arabidopsis dehydrin low temperature tolerance transgenic plants 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Tuula Puhakainen
    • 1
  • Michael W. Hess
    • 2
  • Pirjo Mäkelä
    • 1
    • 3
  • Jan Svensson
    • 1
    • 4
  • Pekka Heino
    • 1
  • E. Tapio Palva
    • 1
  1. 1.Genetics, Department of Biological and Environmental SciencesUniversity of HelsinkiViikki BiocenterFinland
  2. 2.Department of Histology and Molecular Cell biology, Institute of Anatomy, Histology & EmbryologyUniversity of InnsbruckInnsbruckAustria
  3. 3.Department of Applied Biology, Crop ProductionUniversity of HelsinkiFinland
  4. 4.Department of Botany and Plant SciencesUniversity of CaliforniaRiversideUSA

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