Plant Molecular Biology

, Volume 54, Issue 3, pp 387–404 | Cite as

Evidence that CTR1-Mediated Ethylene Signal Transduction in Tomato is Encoded by a Multigene Family Whose Members Display Distinct Regulatory Features

  • Lori Adams-Phillips
  • Cornelius Barry
  • Priya Kannan
  • Julie Leclercq
  • Mondher Bouzayen
  • Jim Giovannoni
Article

Abstract

Ethylene governs a range of developmental and response processes in plants. In Arabidopsis thaliana, the Raf-like kinase CTR1 acts as a key negative regulator of ethylene responses. While only one gene with CTR1 function apparently exists in Arabidopsis, we have isolated a family of CTR1-like genes in tomato (Lycopersicon esculentum). Based on amino acid alignments and phylogenetic analysis, these tomato CTR1-like genes are more similar to ArabidopsisCTR1 than any other sequences in the Arabidopsis genome. Structural analysis reveals considerable conservation in the size and position of the exons between Arabidopsis and tomato CTR1 genomic sequences. Complementation of the Arabidopsisctr1-8 mutant with each of the tomato CTR genes indicates that they are all capable of functioning as negative regulators of the ethylene pathway. We previously reported that LeCTR1 expression is up-regulated in response to ethylene. Here, quantitative real-time PCR was carried out to detail expression for LeCTR1 and the additional CTR1-like genes of tomato. Our results indicate that the tomato CTR1 gene family is differentially regulated at the mRNA level by ethylene and during stages of development marked by increased ethylene biosynthesis, including fruit ripening. The possibility of a multi-gene family of CTR1-like genes in other species besides tomato was examined through mining of EST and genomic sequence databases.

CTR1 ethylene-inducible ethylene signaling MAPKKK negative regulation tomato 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Lori Adams-Phillips
    • 1
    • 2
  • Cornelius Barry
    • 1
  • Priya Kannan
    • 2
  • Julie Leclercq
    • 3
  • Mondher Bouzayen
    • 3
  • Jim Giovannoni
    • 1
    • 4
  1. 1.Boyce Thompson Institute for Plant ResearchCornell UniversityIthacaUSA
  2. 2.Department of Horticultural SciencesTexas A&M UniversityUSA
  3. 3.AuzevilleFrance
  4. 4.USDA-ARS Plant Soil and Nutrition LabCornell UniversityIthacaUSA

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