Plant Molecular Biology

, Volume 54, Issue 1, pp 99–109 | Cite as

Isolation and identification of a gene in response to rice blast disease in rice

  • Xianwu Zheng
  • Xuewei Chen
  • Xiaohong Zhang
  • Zhongzhuan Lin
  • Junjun Shang
  • Jichen Xu
  • Wenxue Zhai
  • Lihuang ZhuEmail author


We combined cDNA amplified fragment length polymorphism (cDNA-AFLP) with bulked segregant analysis (BSA) to detect genes that control rice blast (Magnaporthe grisea) resistance in a double-haploid (DH) population derived from a cross between a blast-resistant variety, Zhai Ye Qing8 (ZYQ8), and a blast-susceptible variety, Jin Xi17 (JX17). In cDNA-AFLP analysis between a blast resistance (R) pool and a blast susceptibility (S) pool from the DH population, 12 transcript-derived fragments (TDFs) that were present in only one of the two pools were detected, 8 of which were from the R pool and 4 from the S pool. Mapping analysis of these TDFs by using the DH mapping population showed that five of them, R1, R8, S9, S16 and S17, were located on chromosome 1. Sequence comparison and allelic analysis showed thatR1/S16 and R8/S9 were two pairs of allelic genes. The full-length cDNA sequences of R1/S16, S17 and R8/S were obtained through cDNA library screening, in which only the expression level of R8 cDNA was up-regulated by inoculation with the blast isolate zh10814 and not affected by mock treatment, suggesting that R8 was implicated in the signaling pathways of the rice blast resistance reaction. Protein function prediction showed that R8 cDNA encodes a protein with high identity to a putative calmodulin-binding protein in Arabidopsis thaliana which belongs to the P-loop-containing nucleotide triphosphate hydrolases superfamily that contains a number of various kinases.

bulked segregant analysis cDNA-AFLP rice blast disease TDFs 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Xianwu Zheng
    • 1
  • Xuewei Chen
    • 1
  • Xiaohong Zhang
    • 1
  • Zhongzhuan Lin
    • 2
  • Junjun Shang
    • 1
  • Jichen Xu
    • 1
  • Wenxue Zhai
    • 1
  • Lihuang Zhu
    • 1
    Email author
  1. 1.Institute of Genetics and Developmental BiologyChinese Academy of ScienceBeijingChina
  2. 2.Institute of Crop Breeding and CultivationChinese Academy of Agriculture SciencesBeijingChina

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