Advertisement

Plant Molecular Biology

, Volume 54, Issue 1, pp 99–109 | Cite as

Isolation and identification of a gene in response to rice blast disease in rice

  • Xianwu Zheng
  • Xuewei Chen
  • Xiaohong Zhang
  • Zhongzhuan Lin
  • Junjun Shang
  • Jichen Xu
  • Wenxue Zhai
  • Lihuang ZhuEmail author
Article

Abstract

We combined cDNA amplified fragment length polymorphism (cDNA-AFLP) with bulked segregant analysis (BSA) to detect genes that control rice blast (Magnaporthe grisea) resistance in a double-haploid (DH) population derived from a cross between a blast-resistant variety, Zhai Ye Qing8 (ZYQ8), and a blast-susceptible variety, Jin Xi17 (JX17). In cDNA-AFLP analysis between a blast resistance (R) pool and a blast susceptibility (S) pool from the DH population, 12 transcript-derived fragments (TDFs) that were present in only one of the two pools were detected, 8 of which were from the R pool and 4 from the S pool. Mapping analysis of these TDFs by using the DH mapping population showed that five of them, R1, R8, S9, S16 and S17, were located on chromosome 1. Sequence comparison and allelic analysis showed thatR1/S16 and R8/S9 were two pairs of allelic genes. The full-length cDNA sequences of R1/S16, S17 and R8/S were obtained through cDNA library screening, in which only the expression level of R8 cDNA was up-regulated by inoculation with the blast isolate zh10814 and not affected by mock treatment, suggesting that R8 was implicated in the signaling pathways of the rice blast resistance reaction. Protein function prediction showed that R8 cDNA encodes a protein with high identity to a putative calmodulin-binding protein in Arabidopsis thaliana which belongs to the P-loop-containing nucleotide triphosphate hydrolases superfamily that contains a number of various kinases.

bulked segregant analysis cDNA-AFLP rice blast disease TDFs 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Altschul, S.F., Gish, W, Miller, W., Myers, E.W., Lipman, D.J. 1990. Basic local alignments search tool. J. Mol. Biol. 215: 403–410.Google Scholar
  2. Arabidopsis Genome Initiative. 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408: 796–815.Google Scholar
  3. Asai, T., Tena, G., Plonikova, J., Willmann, M., Chiu, W.-L., Gomez-Gomez, L. and Sheen, J. 2002. MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415: 977–983.Google Scholar
  4. Austin, M.J., Muskett, P., Kahn, K., Feys, B.J., Jones, J.D.G. and Parker, J.E. 2002. Regulatory role of SGT1 in early R gene-mediated plant defenses. Science 295: 2077–2080.Google Scholar
  5. Bachem, C.W.B., van der Hoeven, R.S., de Bruijn, S.M., Vreugdenhil, D., Zabeau, M. and Visser, R.G.F. 1996. Visualization of differential gene expression using a novel method of RNA fingerprint based on AFLP: analysis of gene expression during potato tuber development. Plant J. 9: 745–753.Google Scholar
  6. Bao, J.S., Zheng, X.W., Xia, Y.W. and Zhu, L.H. 2000. QTL mapping for the paste viscosity characteristics in rice (Oryza sativa L.). Theor. Appl. Genet. 100: 280–284.Google Scholar
  7. Barcaccia, G., Varotto, S., Meneghetti, S., Albertini, E., Porceddu, A., Parrini, P. and Lucchin, M. 2001. Analysis of gene expression during flowering in apomeiotic mutants of Medicago spp.: cloning of ESTs and candidate genes for 2n eggs. Sex. Plant Reprod. 14: 233–238.Google Scholar
  8. Breyne, P. and Zabeau, M. 2001. Genome-wide expression analysis of plant cell cycle modulated genes. Curr. Opin. Plant Biol. 4: 136–142.Google Scholar
  9. Bryan, G.T., Wu, K.-S., Farrall, L., Jia, Y., Hershey, H.P., McAdams, S.A., Faulk, K.N., Donaldson, G.K., Tarchini, R. and Valent, B. 2000. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell 12: 2033–2046.Google Scholar
  10. Cao, H., Glazebrook, J., Clarke, J. D., Volko, S. and Dong, X. 1997 The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats. Cell 88: 57–63.Google Scholar
  11. Century, K.S., Shapiro, A.D., Repetti, P.P., Dahlbeck, D., Holub, E. and Staskawicz, B.J. 1997. NDR1, a pathogen-induced component required for Arabidopsis disease resistance. Science 278: 1963–1965.Google Scholar
  12. Cooper, B. 2001. Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor. Plant J. 6: 339–349.Google Scholar
  13. Dangl, J.L. and Jones, J.D.G. 2001. Plant pathogens and integrated defence responses to infection. Nature 411: 826–833.Google Scholar
  14. Durrant, W.E., Rowland, O., Piedras, P., Hammond-Kosack, K.E. and Jones, J.D.G. 2000. cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles. Plant Cell 12: 963–977.Google Scholar
  15. Flor, H. 1971. Current status of the gene-for-gene concept.Annu. Rev. Phytopath. 9: 275–296.Google Scholar
  16. Glazebrook, J. 2001.. Genes controlling expression of defense responses in Arabidopsis: 2001 status. Curr. Opin. Plant Biol. 4: 301–308.Google Scholar
  17. He, C., Fong, S.H.T., Yang, D. and Wang, G.-L. 1999. BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice. Mol. Plant-Microbe Interact. 12: 1064–1073.Google Scholar
  18. He, P., Shen, L.S., Lu, C.F., Chen, Y. and Zhu, L.H. 1998. Analysis of quantitative trait loci which contribute to anther cultivability in rice (Oryza sativa L.). Mol. Breed. 4: 165–172.Google Scholar
  19. Kagaya, Y., Ohmiya, K. and Hattori T. 1999. RAV1, a novel DNA-binding protein, binding to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plants. Nucl. Acids Res. 27: 470–478.Google Scholar
  20. Kim, M.C., Lee, S.H., Kim, J.K., Chun, H.J., Choi, M.S., Chung, W.S., Byeong, C.M., Kang, C.H., Park, C.Y., Yoo, J.H., Kang, Y.H., Koo, S.C., Koo, Y.D., Jung, J.C., Kim, S.T., Schulze-Lefert, P., Lee, S.Y. and Cho, M.J.. 2002. Mlo, a modulator of plant defense and cell death, is a novel calmodulin-binding protein: isolation and characterization of a rice Mlo homologue. J. Biol. Chem. 277: 19304–19314.Google Scholar
  21. Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Lincoln, S.E. and Etoh, T. 1987. An interactive computer package for constructing primary genetic linkage maps of experimental and natural population. Genomics 1: 174–181.Google Scholar
  22. Ligterink, W., Kroj, T., zur Nieden, U., Hirt, H. and Scheel, D. 1997. Receptor-mediated activation of a MAP kinase in pathogen defense of plants. Science 276: 2054–2057.Google Scholar
  23. Lincoln, S.E., Daly, M.J. and Lander, E.S. 1993a. Constructing genetic linkage maps with MAPMAKER/EXP version 3.0: a tutorial and reference manual, 3rd ed. Whitehead Institute for Biometrical Research, Cambridge, MA.Google Scholar
  24. Liu, Y., Schiff, M., Serino, G., Deng, X.-W. and Dinesh-Kumar, S.P. 2002. Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to tobacco mosaic virus. Plant Cell 14: 1483–1496.Google Scholar
  25. Lu, C.F., Shen, L.S., Tan, Z., Xu, Y.B., He, P., Chen, Y. and Zhu, L.H. 1996. Comparative mapping of QTL for agronomic traits of rice across environment using a doubled haploid population. Theor. Appl. Genet. 93: 1211–1217.Google Scholar
  26. McCouch, S.R., Kochert, G., Yu, Z.Y., Khush, G.S., Coflman, W.R. and Tanksley, S.D. 1988. Molecular mapping of the rice chromosome. Theor. Appl. Genet. 76: 815–819.Google Scholar
  27. McMullen, M.D., Byrne, P.F., Snook M.E., Wiseman, B.R., Lee, E.A., Widstrom, N.W. and Coe, E.H. 1998. Quantitative trait loci and metabolic pathways. Proc. Natl. Acad. Sci. USA 95: 1996–2000.Google Scholar
  28. Michelmore, R.M., Paran, I. and Kesseli, R.V. 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions using segregation populations. Proc. Natl. Acad. Sci. USA 88: 9828–9832.Google Scholar
  29. Nawrath, C., Heck, S., Parinthawong, N. and Métraux, J.-P. 2002. EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family. Plant Cell 14: 275–286.Google Scholar
  30. Ono, E., Wong, H.L., Kawasaki, T., Hasegawa, M., Kodama, O. and Shimamoto, K. 2001. Essential role of the small GTPase Rac in disease resistance of rice. Proc. Natl. Acad. Sci. USA 98: 759–764.Google Scholar
  31. Petersen, M., Brodersen, P., Naested, H., Andreasson, E., Lindhart, U., Johansen, B., Nielsen, H.B., Lacy, M., Austin, M.J, Parker, J.E., Sharma, S. B., Klessig, D. F., Martienssen, R., Mattsson, O., Jensen, A.B. and Mundy, J. 2000. Arabidopsis Map kinase 4 negative regulates systemic acquired resistance. Cell 103: 1111–1120.Google Scholar
  32. Ryals, J. 1993. Requirement of salicylic acid for the induction of systemic acquired resistance. Science 261: 754–756.Google Scholar
  33. Sasaki, T., Takashi, M., Kimiko, Y., Katsumi, S., Masahiro Y., Jiang, J. and Takashi, G. 2002. The genome sequence and structure of rice chromosome 1. Nature 420: 312–316.Google Scholar
  34. Thorup, T.A., Tanyolac, B., Livingstone, K.D., Popovsky, S., Paran, I. and Jahn, M. 2000. Candidate gene analysis of organ pigmentation loci in the Solanaceae. Proc. Natl. Acad. Sci. USA 97: 11192–11197.Google Scholar
  35. van Loon, L.C., Bakker, P.A.H.M. and Pieterse, C.M.J. 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopath. 36: 453–483.Google Scholar
  36. van der Biezen, E., Jones, J.D.G. 1998. Plant disease-resistance proteins and the gene-for-gene concept. Trends Biochem. Sci. 23: 454–456.Google Scholar
  37. Van Deynze, A.E., Sorrells, M.E., Park, W.D., Ayres, N.M., Fu, H., Cartinhour, S.W., Paul, E. and McCouch, S.R. 1998. Anchor probes for comparative mapping of grass genera. Theor. Appl. Genet. 97: 356–369.Google Scholar
  38. Vogel, J.P., Raab, T.K., Schiff, C. and Somerville, S.C. 2002. PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis. Plant Cell 14: 2095–2106.Google Scholar
  39. Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J. and Kuiper, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res. 23: 4407–4414.Google Scholar
  40. Wang, X., Zafian, Choudhari, M. and Lawton, M. 1996. The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins. Proc. Natl. Acad. Sci. USA 93: 2598–2602.Google Scholar
  41. Wang, Z.X., Yano, M., Yamanouchi, U., Iwamoto, M., Monna, L., Hayasaka, H., Katayose, Y. and Sasaki, T. 1999. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J. 19: 55–64.Google Scholar
  42. Wang, Z., Taramino, G., Yang, D., Tingey, S.V., Miao, G.-H. and Wang, G.L. 2001. Rice ESTs with disease-resistance gene-or defense-response gene-like sequences mapped to regions containing major resistance genes or QTL. Mol. Genet. Genomics 265: 302–310.Google Scholar
  43. Wadsworth, G.J., Redinbaugh, M.G. and Scandalios, J.G. 1998. A procedure for small-scale isolation of plant RNA suitable for RNA blot analysis. Anal. Biochem. 172: 279–283.Google Scholar
  44. Xiao, H., Wang, Y, Liu, D.F., Wang, W.M., Li, X.B., Zhao, X.F., Xu, J.C, Zhai, W.X. and Zhu, L.H. 2003. Functional analysis of the rice AP3 homologue OsMADS16 by RNA interference. Plant Mol. Biol. 52: 957–966.Google Scholar
  45. Yu, J., Hu, S.N., Wang, J., et al. 2002. A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science 296: 79–92.Google Scholar
  46. Zhou, N., Tootle, T.L., Tsui, F., Kiessig, D.F. and Glazebrook J. 1998. PAD4 function upstream from salicylic acid to control defense responses in Arabidopsis. Plant Cell 10: 1021–1030.Google Scholar
  47. Zhu, L.H., Chen Y., Xu, Y.B., Xu J.C., Cai, H.W. and Ling, Z.Z. 1993. Construction of a molecular map of rice and gene mapping using a double haploid population of a cross between Indica and Japonica varieties. Rice Genet. Newsl. 10: 132–134.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Xianwu Zheng
    • 1
  • Xuewei Chen
    • 1
  • Xiaohong Zhang
    • 1
  • Zhongzhuan Lin
    • 2
  • Junjun Shang
    • 1
  • Jichen Xu
    • 1
  • Wenxue Zhai
    • 1
  • Lihuang Zhu
    • 1
    Email author
  1. 1.Institute of Genetics and Developmental BiologyChinese Academy of ScienceBeijingChina
  2. 2.Institute of Crop Breeding and CultivationChinese Academy of Agriculture SciencesBeijingChina

Personalised recommendations