, Volume 41, Issue 3, pp 321–330 | Cite as

Chlorophyll Fluorescence as a Tool in Cereal Crop Research

  • O.H. Sayed


Chlorophyll (Chl) fluorescence is a subtle reflection of primary reactions of photosynthesis. Intricate relationships between fluorescence kinetics and photosynthesis help our understanding of photosynthetic biophysical processes. Chl fluorescence technique is useful as a non-invasive tool in eco-physiological studies, and has extensively been used in assessing plant responses to environmental stress. The review gives a summary of some Chl fluorescence parameters currently used in studies of stress physiology of selected cereal crops, namely water stress, heat stress, salt stress, and chilling stress.

barley chilling drought heat maize oat rice sorghum salinity stress wheat 


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  1. Abadia, A., Belkhodja, R., Morales, F., Abadia, J.: Effects of salinity on the photosynthetic pigment composition of barley (Hordeum vulgare L.) grown under a triple-line-source sprinkler system in the field. – J. Plant Physiol. 154: 392–400, 1999.Google Scholar
  2. Aguilera, C., Stirling, C.M., Long, S.P.: Genotypic variation within Zea mays for susceptibility to and rate of recovery from chill-induced photoinhibition of photosynthesis. – Physiol. Plant. 106: 429–436, 1999.Google Scholar
  3. Ali, D.T., Monneveux, P., Acevedo, E., Nachit, M.: Evaluation of proline analysis and chlorophyll fluorescence quenching measurements as drought tolerance indicators in durum wheat (Triticum turgidum L. var. durum). – Euphytica 79: 65–73. 1994.Google Scholar
  4. Andréassson, L.E., Vänngård, T.: Electron transport in photosystems I and II. – Annu. Rev. Plant Physiol. Plant mol. Biol. 39: 379–411, 1988.Google Scholar
  5. Andrews, J.R., Fryer, M.J., Baker, N.B.: Characterization of chilling effects on photosynthetic performance of maize crops during early season growth using chlorophyll fluorescence. – J. exp. Bot. 46: 1195–1203, 1995.Google Scholar
  6. Aroca, R., Irigoyen, J.J., Sánchez-Díaz, M.: Photosynthetic characteristics and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varieties differing in chilling sensitivity. – Plant Sci. 161: 719–726, 2001.Google Scholar
  7. Babani, F., Lichtenthaler, H.K.: Light-induced and age-dependent development of chloroplasts in etiolated barley leaves as visualized by determination of photosynthetic pigments, CO2 assimilation rates and different kinds of chlorophyll fluorescence ratios. – J. Plant Physiol. 148: 555–566, 1996.Google Scholar
  8. Baker, N.R., Bradbury, M., Farage, P.K., Ireland, C.R., Long, S.P.: Measurements of quantum yield of carbon assimilation and chlorophyll fluorescence for assessment of photosynthetic performance of crops in the field. – Phil.Trans. roy. Soc. London B 323: 295–308, 1989.Google Scholar
  9. Balota, M., Lichtenthaler, H.K.: Red chlorophyll fluorescence as an ecophysiological method to assess the behaviour of wheat genotypes under drought and heat. – Cereal Res. Commun. 27: 179–187, 1999.Google Scholar
  10. Belkhodja, R., Morales, F., Abadia, A., Gómez-Aparisi, J., Abadia, J.: Chlorophyll fluorescence as a possible tool for salinity tolerance screening in barley (Hordeum vulgare L.). – Plant Physiol. 104: 667–673, 1994.Google Scholar
  11. Belkhodja, R., Morales, F., Abadía, A., Medrano, H., Abadía, J.: Effects of salinity on chlorophyll fluorescence and photosynthesis of barley (Hordeum vulgare L.) grown under a triple-line-source sprinkler system in the field. – Photosynthetica 36: 375–387, 1999.Google Scholar
  12. Bertin, P., Bouharmont, J., Kinet, J.M.: Somaclonal variation and improvement of chilling tolerance in rice: Changes in chilling-induced chlorophyll fluorescence. – Crop Sci. 37: 1727–1735, 1997.Google Scholar
  13. Bilger, W., Schreiber, U.: Energy-dependent quenching of darklevel chlorophyll fluorescence in intact leaves. – Photosynth. Res. 10: 303–308, 1986.Google Scholar
  14. Bishop, D.G.: Chilling sensitivity in higher plants: The role of phosphatidylglycerol. – Plant Cell Environ. 9: 613–616, 1986.Google Scholar
  15. Blum, A., Johnson, J.W.: Transfer of water from roots into dry soil and the effect on wheat water relations and growth. – Plant Soil 145: 141–149, 1992.Google Scholar
  16. Bolhàr-Nordenkampf, H.R., Öquist, G.O.: Chlorophyll fluorescence as a tool in photosynthesis research. – In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegoood, R.C., Long, S.P. (ed.): Photosynthesis and Production in a Changing Environment. A Field and Laboratory Manual. Pp. 193–206. Chapman & Hall, London – Glasgow – New York – Tokyo – Melbourne – Madras 1993.Google Scholar
  17. Bradbury, M., Baker, N.R.: Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of photosystem II electron acceptors and fluorescence emission from photosystems I and II. – Biochim. biophys. Acta 63: 542–551, 1981.Google Scholar
  18. Bradford, K.J., Hsiao, T.C.: Ecophysiological responses to moderate water stress. – In: Encyclopedia of Plant Physiology. Vol. 12B. Pp. 232–253. Springer-Verlag, Berlin 1982.Google Scholar
  19. Briantais, J.-M, Vernotte, C., Picaud, M., Krause, G.H.: A quantitative study of the slow decline of chlorophyll a fluorescence in isolated chloroplasts. – Biochim. biophys. Acta 548: 128–138, 1979.Google Scholar
  20. Buchanan, B.B., Gruissem, W., Jones, R.L.: Biochemistry and Molecular Biology of Plants. – Amer. Soc. Plant Physiol., Rockville 2000.Google Scholar
  21. Bukhov, N.G., Boucher, N., Carpentier, R.: After effect of short-term heat shock on photosynthetic reactions in barley leaves. – Fiziol. Rast. 44: 605–612, 1997.Google Scholar
  22. Buschmann, C.: Photochemical and non-photochemical quenching coefficients of the chlorophyll fluorescence: comparison of variation and limits. – Photosynthetica 37: 217–224, 1999.Google Scholar
  23. Buschmann, C., Langsdorf, G., Lichtenthaler, H.K.: Imaging of the blue, green and red fluorescence emission of plants: An overview. – Photosynthetica 38: 483–491, 2000.Google Scholar
  24. Butler, W.L.: Chlorophyll fluorescence: A probe for electron transfer and energy transfer. – In: Trebst, A., Avron, M. (ed.): Photosynthesis I. Pp. 149–166. Springer-Verlag, Berlin – Heidelberg – New York 1977.Google Scholar
  25. Cave, G.: Water and membranes: The interdependence of their physico-chemical properties in the case of phospholipid head groups. – Stud. biophys. 91: 41–46, 1981.Google Scholar
  26. Cogdell, R.J.: Photosynthetic reaction centers. – Annu. Rev. Plant Physiol. 34: 21–45, 1983.Google Scholar
  27. Corlett, J.E., Jones, H.G., Massacci, A., Masojidek, J.: Water deficit, leaf rolling and susceptibility to photoinhibition in field grown sorghum. – Physiol. Plant. 92: 423–430, 1994.Google Scholar
  28. Crafts-Brandner, S.J., Salvucci, M.E.: Rubisco activase constrains the photosynthetic potential of leaves at high temperature. – Proc. nat. Acad. Sci. USA 97: 13430–13435, 2000.Google Scholar
  29. Crafts-Brandner, S.J., van den Loo, F.J., Salvucci, M.E.: The two forms of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase differ in sensitivity to elevated temperatures. – Plant Physiol. 114: 439–444, 2000.Google Scholar
  30. Cramer, G.R., Bowman, D.C.: Kinetics of maize leaf elongation. I. Increased yield threshold limits short term steady state elongation rates after exposure to salinity. – J. exp. Bot. 42: 1417–1426, 1991.Google Scholar
  31. Cramer, G.R., Epstein, E., Läuchli, A.: Kinetics of root elongation of maize in response to short-term exposure to NaCl and elevated calcium concentration. – J. exp. Bot. 39: 1573–1582, 1988.Google Scholar
  32. Crowe, J.H., Crowe, L.M., Chapman, D.: Preservation of membranes in anhydrobiotic organisms. Role of trehalose. – Science 223: 701–703, 1984.Google Scholar
  33. Daley, P.F.: Chlorophyll fluorescence analysis and imaging in plant stress and disease. – Can. J. Plant Pathol. 17: 167–173, 1995.Google Scholar
  34. Dash, S., Mohanty, N.: Evaluation of assays for the analysis of thermo-tolerance and recovery potentials of seedlings of wheat (Triticum aestivum L.) cultivars. – J. Plant Physiol. 158: 1153–1165, 2001.Google Scholar
  35. Davies, W.J., Schurr, U., Taylor, G., Zhang, J.: Hormones as chemical signals involved in root to shoot communication of effect of changes in the soil environment. – In: Hoad, G.U., Lenton, J.R., Atkin, R. (ed.): Hormone Action in Plant Development. Pp. 201–206. Butterworth, London 1987.Google Scholar
  36. Davies, W.J., Zhang, J.: Root signals and the regulation of growth and development in plants in drying soils. – Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 55–70, 1991.Google Scholar
  37. Demmig-Adams, B., Adams, W.W., III: Photoprotection and other responses of plants to high light stress. – Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 599–626, 1992.Google Scholar
  38. Dionisio-Sese, M.L., Tobita, S.: Effects of salinity on sodium content and photosynthetic responses of rice seedlings differing in salt tolerance. – J. Plant Physiol. 157: 54–58, 2000.Google Scholar
  39. Dory, I., Boddi, B., Kissimon, J., Paldi, E.: Cold stress responses of inbred maize lines with various degrees of cold tolerance. – Acta agron. hung. 39: 309–318, 1990.Google Scholar
  40. Earla, H.J., Tollenaarb, M.: Using chlorophyll fluorometry to compare photosynthetic performance of commercial maize (Zea mays L.) hybrids in the field. – Field Crops Res. 61: 201–210, 1999.Google Scholar
  41. Eckardt, N.A., Portis, A.R.: Heat denaturation profiles of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and Rubisco activase and the inability of Rubisco activase to restore activity of heat-denatured Rubisco. – Plant Physiol. 113: 243–248, 1997.Google Scholar
  42. El-Shintinawy, F.: Photosynthesis in two wheat cultivars differing in salt susceptibility. – Photosynthetica 38: 615–620, 2000.Google Scholar
  43. Fedina, I.S., Georgieva, K., Grigorova, I.: Light-dark changes in proline content of barley leaves under salt stress. – Biol. Plant. 45: 59–63, 2002.Google Scholar
  44. Feller, U., Crafts-Brandner, S.J., Salvucci, M.E.: Moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase mediated activation of Rubisco. – Plant Physiol. 116: 539–546, 1998.Google Scholar
  45. Flagella, Z., Campanile, R.G., Ronga, G., Stoppelli, M.C., Pastore, D., De Caro, A., Di Fonzo, N.: The maintenance of photosynthetic electron transport in relation to osmotic adjustment in durum wheat cultivars differing in drought resistance. – Plant Sci. 118: 127–133, 1996.Google Scholar
  46. Flagella, Z., Pastore, D., Campanile, R.G., Di Fonzo, N.: Photochemical quenching of chlorophyll fluorescence and drought tolerance in different durum wheat (Triticum durum) cultivars. – J. agr. Sci. 122: 183–192, 1994.Google Scholar
  47. Fracheboud, Y., Haldimann, P., Leipner, J., Stamp, P.: Chlorophyll fluorescence as a selection tool for cold tolerance of photosynthesis in maize (Zea mays L.). – J. exp. Bot. 50: 1533–1540, 1999.Google Scholar
  48. Frensch, J.: Primary responses of root and leaf elongation to water deficits in the atmosphere and soil solution. – J. exp. Bot. 48: 985–999, 1997.Google Scholar
  49. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. – Biochim. biophys. Acta 990: 87–92, 1989.Google Scholar
  50. Glazer, A.N., Melis, A.: Photochemical reaction centers: structure, organization, and function. – Annu. Rev. Plant Physiol. 38: 11–45, 1987.Google Scholar
  51. Goedheer, J.C.: Fluorescence in relation to photosynthesis. – Annu. Rev. Plant Physiol. 23: 87–112, 1972.Google Scholar
  52. Govindjee: Sixty-three years since Kautsky: Chlorophyll a fluorescence. – Aust. J. Plant Physiol. 22: 131–160, 1995.Google Scholar
  53. Greer, D.H., Hardacre, A.K.: Photoinhibition of photosynthesis and its recovery in two maize hybrids varying in low temperature tolerance. – Aust. J. Plant Physiol. 16: 189–198, 1989.Google Scholar
  54. Guenther, J.E., Melis, A.: Dynamics of photosystem II heterogeneity in Dunaliella salina (green algae). – Photosynth. Res. 23: 195–203, 1990.Google Scholar
  55. Guisse, B., Srivastava, A., Strasser, R.J.: The polyphasic rise of the chlorophyll a fluorescence (O-K-J-I-P) in heat-stressed leaves. – Arch. Sci. 48: 147–160, 1995.Google Scholar
  56. Guye, M.G., Wilson, J.M.: The effects of chilling and chill-hardening temperatures on stomatal behaviour in a range of chilling-sensitive species and culturivars. – Plant Physiol. Biochem. 25: 717–721, 1987.Google Scholar
  57. Haitz, M, Lichtenthaler, H.K: The measurement of Rfd-values as plant vitality indices with the portable field fluorometer and the PAM-fluorometer. – In: Lichtenthaler, H.K. (ed.): Applications of Chlorophyll Fluorescence. Pp. 249–254. Kluwer Academic Publishers, Dordrecht – Boston – London 1988.Google Scholar
  58. Haldimann, P., Fracheboud, Y., Stamp, P.: Photosynthetic performance and resistance to photoinhibition of Zea mays. leaves grown at sub-optimal temperature. – Plant Cell Environ. 19: 85–92, 1996.Google Scholar
  59. Havaux, M., Ernez, M., Lannoye, R.: FrSélection de variétés de blé dur (Triticum durum Desf.) and de blé tendre (Triticum aestivum L.) adaptées à la sécheresse par la mesure de l'extinction de la fluorescence de la chlorophylle in vivo. – Agronomie 8: 193–199, 1988.Google Scholar
  60. Herzog, H., Olszewski, A.: A rapid method for measuring freezing resistance in crop plants. – J. Agron. Crop Sci. 181: 71–79, 1998.Google Scholar
  61. Hetherington, S.E., Öquist, G.: Monitoring chilling injury: comparison of chlorophyll fluorescence measurements, post-chilling growth and visible symptoms of injury in Zea mays. – Physiol. Plant. 72: 241–247, 1988.Google Scholar
  62. Holzwarth, A.R.: Excited-state kinetics in chlorophyll systems and its relationship to the functional organization of the photosystems. – In: Scheer, H. (ed.): Chlorophylls. Pp. 1125–1151. CRC Press, Boca Raton – Ann Arbor – Boston – London 1991.Google Scholar
  63. Hong, S.-S., Hong, T., Jiang, H., Xu, D.-Q.: Changes in the non-photochemical quenching of chlorophyll fluorescence during aging of wheat flag leaves. – Photosynthetica 36: 621–625, 1999.Google Scholar
  64. Hormann, H., Neubauer, C., Schreiber, U.: On the relationship between chlorophyll fluorescence quenching and the quantum yield of electron transport in isolated thylakoids. – Photosynth. Res. 40: 93–106, 1994.Google Scholar
  65. Horton, P., Bowyer, J.: Chlorophyll fluorescence transients. – In: Harwood, J., Bowyer, J.R. (ed.): Methods in Plant Biochemistry. Pp. 259–296. Academic Press, London 1990.Google Scholar
  66. Hsiao, T.C.: Plant responses to water stress. – Annu. Rev. Plant Physiol. 24: 519–570, 1973.Google Scholar
  67. Hume, D.J., Jackson, A.K.: Pod formation in soybeans at low temperature. – Genetica 31: 1–20, 1981.Google Scholar
  68. Ilík, P., Kouril, R., Fiala, J., Nauš, J., Vacha, F.: Spectral characterization of chlorophyll fluorescence in barley leaves during linear heating. Analysis of high-temperature fluorescence rise around 60ºC. – J. Photochem. Photobiol. 59: 103–114, 2000.Google Scholar
  69. Ingram, J., Bartels, D.: The molecular basis of dehydration tolerance in plants. – Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 377–403, 1996.Google Scholar
  70. Janowiak, F., Adamczyk, J., Krolikowski, Z.: Differentiation of chilling tolerance among Polish maize lines as measured by chlorophyll fluorescence method. – In: Proc. 3rd Int. Congress Ecophysiological Aspects of Plant Responses to Stress Factors. Kraków 2000.Google Scholar
  71. Jin, M.-X., Li, D.-Y., Mi, H.: Effects of high temperature on chlorophyll fluorescence induction and the kinetics of far red radiation-induced relaxation of apparent F0 in maize leaves. – Photosynthetica 40: 581–586, 2002.Google Scholar
  72. Jiao, D.-M., Li, X., Huang, X.-Q., Chi, W., Kuang, T.-Y., Zhang, Q.-D., Ku, M.S.B.: Characteristics of carbon assimilation and chlorophyll fluorescence in C4 photosynthetic enzymes transgenic rice. – Photosynth. Res. 69: 238, 2001.Google Scholar
  73. Jones, C.A.: C4 Grasses and Cereals. – John Wiley & Sons, New York 1985.Google Scholar
  74. Joshi, M.K., Mohanty, P.: Probing photosynthetic performance by chlorophyll a fluorescence: Analysis and interpretation of fluorescence parameters. – J. sci. ind. Res. 54: 155–174, 1995.Google Scholar
  75. Jovanovic, L., Veljovic, S., Janjic, V.: Water regime and photosynthesis parameters in two maize lines differing in drought susceptibility. – Biol. Vest. 39: 103–108, 1991.Google Scholar
  76. Kautsky, H., Appel, W., Amann, H.: Chlorophyllfluoreszenz und Kohlensäureassimilation. – Biochem. Z. 322: 277–292, 1960.Google Scholar
  77. Kicheva, M.I., Tsonev, T.D., Popova, L.P.: Stomatal and nonstomatal limitations to photosynthesis in two wheat cultivars subjected to water stress. – Photosynthetica 30: 107–116, 1994.Google Scholar
  78. Kima, J.H., Hwanga, H.J., Parka, H.S., Leeb, C.B., Myungc, K.Y., Lee, C.H.: Differences in the rate of dephosphorylation of thylakoid proteins during dark incubation after chilling in the light between two rice (Oryza sativa L.) varieties. – Plant Sci. 128: 159–168, 1997.Google Scholar
  79. Kitajima, M., Butler, W.L.: Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. – Biochim. biophys. Acta 376: 105–115, 1975.Google Scholar
  80. Klinkovský, T., Nauš, J.: Sensitivity of the relative Fpl level of chlorophyll fluorescence induction in leaves to the heat stress. – Photosynth. Res. 39: 201–204, 1994.Google Scholar
  81. Koscielniak, J., Biesaga-Koscielniak, J.: Effects of exposure to short periods of suboptimal temperature during chill (5C) on gas exchange and chlorophyll fluorescence in maize seedlings (Zea mays L.). – J. Agron. Crop Sci. 183: 231–241, 1999.Google Scholar
  82. Kramer, D.M., DiMarco, G., Loreto, F.: Contribution of plastoquinone quenching to saturation pulse-induced rise of chlorophyll fluorescence in leaves. – In: Mathis, P. (ed.): Photosynthesis: From Light to Biosphere. Vol.I. Pp. 147–150. Kluwer Scientific Publishers, Dordrecht – Boston – London 1995.Google Scholar
  83. Krause, G.H, Weis, E.: Chlorophyll fluorescence and photosynthesis. The basics. – Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313–349, 1991.Google Scholar
  84. Krishnaraj, S., Mawson, B.T., Yeung, E.C., Thorpe, T.A.: Utilization of induction and quenching kinetics of chlorophyll a fluorescence for in vivo salinity screening studies in wheat (Triticum aestivum vars. Kharchia-65 and Fielder). – Can. J. Bot. 71: 87–92, 1993.Google Scholar
  85. Lafitte, H.R., Edmeades, G.O.: Temperature effects on radiation use and biomass partitioning in diverse maize cultivars. – Field Crops Res. 49: 231–247, 1997.Google Scholar
  86. Lazár, D.: Chlorophyll a fluorescence induction. – Biochim. biophys. Acta 1412: 1–28, 1999.Google Scholar
  87. Lazár, D., Ilík, P.: High-temperature induced chlorophyll fluorescence changes in barley leaves. Comparison of the critical temperatures determined from fluorescence induction and from fluorescence temperature curve. – Plant Sci. 124: 159–164, 1997.Google Scholar
  88. Leipner, J., Fracheboud, Y., Stamp, P.: Acclimation by suboptimal growth temperature diminishes photooxidative damage in maize leaves. – Plant Cell Environ. 20: 366–372, 1997.Google Scholar
  89. Leshem, Y.Y.: Plant Membranes. Biophysical Approach to Membrane Structure and Function. – Kluwer Academic Publishers, Dordrecht 1997.Google Scholar
  90. Lichtenthaler, H.K.: In vivo chlorophyll fluorescence as a tool for stress detection in plants. – In: Lichtenthaler, H.K. (ed.): Applications of Chlorophyll Fluorescence. Pp. 129–142. Kluwer Academic Publishers, Dordrecht – Boston – London 1988.Google Scholar
  91. Lichtenthaler, H.K.: Applications of chlorophyll fluorescence in stress physiology and remote sensing. – In: Steven, M.D., Clark, J.A. (ed.): Applications of Remote Sensing in Agriculture. Pp. 287–305. Butterworth Scientific, London 1990.Google Scholar
  92. Lichtenthaler, H.K.: The Kautsky effect: 60 years of chlorophyll fluorescence induction kinetics. – Photosynthetica 27: 45–55, 1992.Google Scholar
  93. Lichtenthaler, H.K, Babani, F.: Detection of photosynthetic activity and water stress by imaging the red chlorophyll fluorescence. – Plant Physiol. Biochem. 38: 889–895, 2000.Google Scholar
  94. Lichtenthaler, H.K., Babani, F., Langesdorf, G., Buschmann, C.: Measurement of differences in red chlorophyll fluorescence and photosynthetic activity between sun and shade leaves by fluorescence imaging. – Photosynthetica 38: 521–529, 2000.Google Scholar
  95. Lichtenthaler, H.K., Burkart, S., Schindler, C., Stober, F.: Changes in photosynthetic pigments and in vivo chlorophyll fluorescence parameters under photoinhibitory growth conditions. – Photosynthetica 27: 343–353, 1992.Google Scholar
  96. Lichtenthaler, H., Buschmann, C., Rinderle, U., Schmuck, G.: Application of chlorophyll fluorescence in ecophysiology. – Radiat. environ. Biophys. 25: 297–308, 1986.Google Scholar
  97. Lichtenthaler, H.K., Miehé, J.A.: Fluorescence imaging as a diagnostic tool for plant stress. – Trends Plant Sci. 2: 316–320, 1997.Google Scholar
  98. Lichtenthaler, H.K., Rinderle, U.: The role of chlorophyll fluorescence in the detection of stress conditions in plants. – CRC crit. Rev. anal. Chem. 19: S29-S85, 1988.Google Scholar
  99. Lichtenthaler, H.K., Wenzel, O., Buschmann, C., Gitelson, A.: Plant stress detection by reflectance and fluorescence. – Ann. New York Acad. Sci. 851: 271–285, 1998.Google Scholar
  100. Lu, C., Zhang, J.: Effects of water stress on photosynthesis, chlorophyll fluorescence and photoinhibition in wheat plants. – Aust. J. Plant Physiol. 25: 883–892, 1998.Google Scholar
  101. Lu, C., Zhang, J.: Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. – J. exp. Bot. 50: 1199–1206, 1999.Google Scholar
  102. Lutts, S., Kinet, J.M., Bouharmont, J.: NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. – Ann. Bot. 78: 389–398, 1996.Google Scholar
  103. Masojídek, J., Trivedi, S., Halshaw, L., Alexiou, A., Hall, D.O.: Synergistic effect of drought and light stresses in sorghum and pearl millet. – Plant Physiol. 96: 198–207, 1991.Google Scholar
  104. Matoušková, M., Bartošková, H., Nauš, J., Novotný, R.: Reaction of photosynthetic apparatus to dark desiccation sensitively detected by the induction of chlorophyll fluorescence quenching. – J. Plant Physiol. 155: 399–406, 1999.Google Scholar
  105. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence: A practical guide. – J. exp. Bot. 345: 659–668, 2002.Google Scholar
  106. McKersie, B.D., Leshem, Y.Y.: Stress and Stress Coping in Cultivated Plants. – Kluwer Scientific Publishers, Dordrecht 1994.Google Scholar
  107. McWilliam, J.R., Kramer, P.J., Musser, R.L.: Temperature induced water stress in chilling-sensitive plants. – Aust. J. Plant Physiol. 9: 343–352, 1982.Google Scholar
  108. Mishra, R.K., Singhal, G.S.: Photosynthetic activity and peroxidation of thylakoid lipids during photoinhibition and high temperature treatment of isolated wheat chloroplasts. – J. Plant Physiol. 141: 286–292, 1993.Google Scholar
  109. Moffatt, J.M., Sears, R.G., Cox, T.S., Paulsen, G.M.: Wheat high temperature tolerance during reproductive growth II. Genetic analysis of chlorophyll fluorescence. – Crop Sci. 30: 886–889, 1990.Google Scholar
  110. Mohammed, G.H., Binder, W.D., Gillies, S.L.: Chlorophyll fluorescence: A review of its practical forestry applications and instrumentation. – Scand. J. Forest Res. 10: 383–410, 1995.Google Scholar
  111. Mohammad, H.T., Sayed, O.H.: Rescheduling maize irrigation for water conservation. I. Chlorophyll fluorescence, cultivar screening, and yield assessment. – Bull. Fac. Sci. (Assiut Univ., Egypt) 31: 35–41, 2002.Google Scholar
  112. Mooney, H.A., Winner, W.E., Pell, E.J., Chu, E.: Responses of Plants to Multiple Stress. – Academic Press, London 1991.Google Scholar
  113. Murata, N.: Molecular species composition of phosphatidylglycerols from chilling-sensitive and chilling-resistant plants. – Plant Cell Physiol. 25: 1241–1245, 1983.Google Scholar
  114. Nauš, J., Kuropatwa, R., Klinkovský, T., Ilik, P., Lattová, J., Pavlová, Z.: Heat injury of barley leaves detected by the chlorophyll fluorescence temperature curve. – Biochim. biophys. Acta 1101: 359–362, 1992.Google Scholar
  115. Neubauer, C., Schreiber, U.: The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination. I. Saturation characteristics and partial control by the photosystem II acceptor side. – Z. Naturforsch. 42c: 1246–1254, 1987.Google Scholar
  116. Newman, P.M.: Wall extensibility and the growth of salt stressed plants. – In: Jackson, M.B., Black, C.R. (ed.): Interacting Stresses in Plants in Changing Environments. Pp. 603–615. Springer-Verlag, Berlin 1993.Google Scholar
  117. Nilsen, E.T., Orcutt, D.M.: The Physiology of Plants Under Stress. Abiotic Factors. – John Wiley & Sons, New York 1999.Google Scholar
  118. Nogués, S., Alegre, L., Araus, J., Perez-Aranda, L., Lannoye, R.: Modulated chlorophyll fluorescence and photosynthetic gas exchange as rapid screening methods for drought tolerance in barley genotypes. – Photosynthetica 30: 465–474, 1994.Google Scholar
  119. Nyachiro, J.M., Briggs, K.G., Hoddinott, J., Johnson-Flanagan, A.M.: Chlorophyll content, chlorophyll fluorescence and water deficit in spring wheat. – Cereal Res. Commun. 29: 135–142, 2001.Google Scholar
  120. Park, Y.I., Park, M.C., Hong, Y.N.: Correlative changes between photosynthetic activities and chlorophyll fluorescence in wheat chloroplasts exposed to high temperature. – J. Plant Biol. 37: 37–42, 1994.Google Scholar
  121. Pasda, G., Diepenbrock, W.: Effects of chilling and genotype on membrane lipids and membrane-dependent characteristics in leaves of maize (Zea mays L.): II. Movements of the plastoquinone-pool measured with the chlorophyll fluorescence technique. – Kuehn Arch. 90: 209–224, 1996.Google Scholar
  122. Pastore, D., Flagella, Z., Rascio, A., Cedola, M., Wittmer, G.: Field studies on chlorophyll fluorescence as drought test in Triticum durum Desf. genotypes. – J. Genet. Breed.43: 45–52, 1989.Google Scholar
  123. Polyakoff-Mayber, A., Lerner, H.R.: Plants in saline environments. – In: Pessarakli, M. (ed.): Handbook of Plant Stress. Pp. 245–278. Marcell Dekker, New York 1994.Google Scholar
  124. Pospíšil, P.: Mechanisms of non-photochemical chlorophyll fluorescence quenching in higher plants. – Photosynthetica 34: 343–355, 1997.Google Scholar
  125. Quinn, P.J., Williams, W.P.: Environmentally induced changes in chloroplast membranes and their effects on photosynthetic function. – In: Barber, J., Baker, N.R. (ed.): Photosynthetic Mechanisms and the Environment. Pp. 1–47. Elsevier, Amsterdam – New York – Oxford 1985.Google Scholar
  126. Rascher, U., Liebig, M., Lüttge, U.: Evaluation of instant lightresponse curves of chlorophyll fluorescence parameters obtained with a portable chlorophyll fluorometer on site in the field. – Plant Cell Environ. 23: 1397–1405, 2000.Google Scholar
  127. Rekika, D., Kara, Y., Souyris, I., Nachit, M., Asbati, A., Monneveux, P.: The tolerance of PSII to high temperatures in durum wheat (T. turgidum conv. durum): Genetic variation and relationship with yield under heat stress. – Cereal Res. Commun. 28: 395–402, 2002.Google Scholar
  128. Rinderle, U., Lichtenthaler, H.K.: The chlorophyll fluorescence ratio F690/F735 as a possible stress indicator. – In: Lichtenthaler, H.K. (ed): Applications of Chlorophyll Fluorescence. Pp. 189–196. Kluwer Academic Publishers, Dordrecht – Boston – London 1988.Google Scholar
  129. Roháček, K.: Chlorophyll fluorescence parameters: the definitions, photosynthetic meaning and mutual relationships. – Photosynthetica 40: 13–29, 2002.Google Scholar
  130. Roháček, K., Barták, M.: Technique of the modulated chlorophyll fluorescence: basic concepts, useful parameters, and some applications. – Photosynthetica 37: 339–363, 1999.Google Scholar
  131. Saccardy, K., Pineau, B., Roche, O., Cornic, G.: Photochemical efficiency of Photosystem II and xanthophyll cycle components in Zea mays leaves exposed to water stress and high light. – Photosynth. Res. 56: 57–66, 1998.Google Scholar
  132. Samson, G., Prášil, O., Yaakoubd, B.: Photochemical and thermal phases of chlorophyll a fluorescence. – Photosynthetica 37: 163–182, 1999.Google Scholar
  133. Sayed, O.H.: Photosynthetic acclimation to high temperature in wheat. – Acta bot. neerl. 41: 299–304, 1992.Google Scholar
  134. Sayed, O.H.: Aridity and plant survival in desert environments. – In: Prakash, I. (ed.): Ecology of Desert Environments. Pp. 87–103. Scientific Publishers, Jodhpur 2001.Google Scholar
  135. Sayed, O.H., Earnshaw, M.J., Emes, M.J.: Photosynthetic responses of different varieties of wheat to high temperature. II. Effect of heat stress on photosynthetic electron transport. – J. exp. Bot. 40: 633–638, 1989a.Google Scholar
  136. Sayed, O.H., Earnshaw, M.J., Emes, M.J.: Characterization of the heat-induced stimulation of Photosystem-I-mediated electron transport. – Acta bot. neerl. 43: 137–143, 1994.Google Scholar
  137. Sayed, O.H., Emes, M.J., Butler, R.D., Earnshaw, M.J.: High temperature induced changes in chloroplast ultrastructure, leaf fluorescence, and photosynthesis in wheat varieties. – Biochem. Soc. Trans. 14: 59, 1986.Google Scholar
  138. Sayed, O.H., Emes, M.J., Earnshaw, M.J., Butler, R.D.: Photosynthetic responses of different varieties of wheat to high temperature. I. Effect of growth temperature on development and photosynthetic performance. –J. exp. Bot. 40: 625–631, 1989b.Google Scholar
  139. Schapendonk, A.H.C.M., Dolstra, O., Van Kooten, O.: The use of chlorophyll fluorescence as a screening method for cold tolerance in maize. – Photosynth. Res. 20: 235–247, 1989.Google Scholar
  140. Schreiber, U.: Chlorophyll fluorescence: New instruments for special applications. – In: Garab, G. (ed.): Photosynthesis: Mechanisms and Effects. Vol. V. Pp. 4253–4258. Kluwer Academic Publishers, Dordrecht – Boston – London 1998.Google Scholar
  141. Schreiber, U., Bilger, W.: Progress in chlorophyll fluorescence research: major developments during the past years in retrospect. – Progress Bot. 54: 151–173, 1993.Google Scholar
  142. Schreiber, U., Bilger, W.: Rapid assessment of stress effects on plant leaves by chlorophyll fluorescence measurements. – In: Tenhunen, J.D., Catarino, F.M., Lange, O.L., Oechel, W.C. (ed.): Plant Responses to Stress. Pp. 27–53. Springer-Verlag, Berlin 1998.Google Scholar
  143. Schreiber, U., Bilger, W., Hormann, H., Neubauer, C.: Chlorophyll fluorescence as a diagnostic tool: Basics and some aspects of practical relevance. – In: Raghavendra, A.S. (ed.): Photosynthesis. Comprehensive Treatise. Pp. 320–336. Cambridge University Press, Cambridge 2000.Google Scholar
  144. Schreiber, U., Neubauer, C.: O2-dependent electron flow, membrane energization and the mechanism of non-photochemical quenching of chlorophyll fluorescence. – Photosynth. Res. 25: 279–293, 1990.Google Scholar
  145. Schulze, E.-D.: Carbon dioxide and water vapor exchange in response to drought in the atmosphere and in the soil. – Annu. Rev. Plant Physiol. 37: 247–274, 1986.Google Scholar
  146. Selmani, A., Wassom, C.E.: Effect of mild drought on chlorophyll fluorescence and morphological traits in young maize seedlings. – Trans. Kansas Acad. Sci. 94: 85–94, 1991.Google Scholar
  147. Šesták, Z., Šiffel, P.: Leaf-age related differences in chlorophyll fluorescence. – Photosynthetica 33: 347–369, 1997.Google Scholar
  148. Shangguan, Z., Shao, M.G., Dyckmans, J.: Effects of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat. – J. Plant Physiol. 156: 46–51,2000.Google Scholar
  149. Sharma, P.K., Hall, D.O.: Changes in carotenoid composition and photosynthesis in sorghum under high light and salt stresses. – J. Plant Physiol. 140: 661–666, 1992.Google Scholar
  150. Sharp, R.E., Davies, W.J.: Regulation of growth and development of plants growing with a restricted supply of water. – In: Hamlyn, G.J., Flowers, T.J., Jones, M.B. (ed.): Plants Under Stress. Pp. 71–93. Cambridge University Press, Cambridge 1989.Google Scholar
  151. Shpiler, L., Blum, A.: Heat tolerance for yield and its components in different wheat culrivars. – Euphytica 51: 257–263, 1991.Google Scholar
  152. Sicher, R.C., Sundblad, L.-G., Öquist, G.: Effects of low temperature acclimation upon photosynthetic induction in barley primary leaves. – Physiol. Plant. 73: 206–210, 1988.Google Scholar
  153. Somersalo, S., Krause, G.H.: Reversible photoinhibition of unhardened and cold acclimated spinach leaves at chilling temperatures. – Planta 180: 181–187, 1990.Google Scholar
  154. Stahl, U., Tusov, V.B., Paschenko, V.Z., Voigt, J.: Spectroscopic investigations of fluorescence behaviour, role and function of the long-wavelength pigments of Photosystem I. – Biochim. biophys. Acta 973: 198–200, 1989.Google Scholar
  155. Sthapit, B.R., Wilson, J.: Chilling tolerance in February seeded Chaite rices (Oryza sativa L.) of Nepal. – Ann. appl. Biol. 121: 189–197, 1992.Google Scholar
  156. Strasser, R.J., Srivastava, A., Govindjee: Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. – Photochem. Photobiol. 61:32–42, 1995.Google Scholar
  157. Stirbet, A., Govindjee, Strasser, B.J., Strasser, R.J.: Chlorophyll a fluorescence induction in higher plants: Modelling and numerical simulation. – J. theor. Biol. 193: 131–151, 1998.Google Scholar
  158. Tambussi, E.A., Casadesus, J., Munné-Bosch, S., Araus, J.L.: Photoprotection in water-stressed plants of durum wheat (Triticum turgidum var. durum): changes in chlorophyll fluorescence, spectral signature and photosynthetic pigments. – Funct. Plant Biol. 29: 35–44, 2002.Google Scholar
  159. Teicher, H.B., Møller, B.L., Scheller, H.V.: Photoinhibition of Photosystem I in field-grown barley (Hordeum vulgare L.): Induction, recovery and acclimation. – Photosynth. Res. 64: 53–61, 2000.Google Scholar
  160. Teiz, L., Zeiger, E.: Plant Physiology. – Benjamin-Cummings Publishers, New York 1991.Google Scholar
  161. Tjus, S.E., Møller, B.L., Scheller, H.V.: Photosystem I is an early target of photoinhibition in barley illuminated at chilling temperatures. – Plant Physiol. 116: 755–764, 1998.Google Scholar
  162. Tjus, S.E., Møller, B.L., Scheller, H.V.: Photoinhibition of Photosystem I damages both reaction centre proteins PSI-A and PSI-B and acceptor-side located small Photosystem I polypeptides. – Photosynth. Res. 60: 75–86, 1999.Google Scholar
  163. Tomek, P., Lazár, D., Ilík, P., Nauš, J.: On the intermediate steps between the O and P steps in chlorophyll a fluorescence rise measured at different intensities of exciting light. – Aust. J. Plant Physiol. 28: 1151–1160, 2001.Google Scholar
  164. van der Veen, R.: Fluorescence and induction phenomena in photosynthesis. – Physiol. Plant. 4: 486–494, 1951.Google Scholar
  165. van Kooten, O., Snel, J.F.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. – Photosynth. Res. 25: 147–150, 1990.Google Scholar
  166. Verheul, M.J., Van Hassel, P.R., Stamp, P.: Comparison of maize inbred lines differing in low temperature tolerance: Effect of acclimation at suboptimal temperature on chloroplast functioning. – Ann. Bot. 76: 7–14, 1995.Google Scholar
  167. Waisel, Y.: Adaptation to salinity. – In: Raghavendra, A.S. (ed.): Physiology of Stress. Pp. 359–383. John Wiely & Sons, New York 1991.Google Scholar
  168. Waisel, Y., Eshel, A., Kafkafi, U.: Plant Roots. The Hidden Part. – Marcel Dekker, New York 1991.Google Scholar
  169. Walker, D.A.: Measurement of oxygen and chlorophyll fluorescence measurement. – In: Coombs, J., Hall, D.O., Long, S.P., Scurlock, J.M. (ed.): Techniques in Bioproductivity and Photosynthesis. 2nd Ed. Pp. 95–106. Pergamon Press, Oxford – New York – Sydney – Frankfurt 1985.Google Scholar
  170. Warrington, I.J., Dunstone, R.I., Green, L.M.: Temperature effects at three developmental stages on the yield of the wheat ear. – Aust. J. agr. Res. 28: 11–27, 1977.Google Scholar
  171. Xu, X.L., Wang, Z.M., Zhang, J.P.: Effect of heat stress on photosynthetic characteristics of different green organs of winter wheat during grain-filling stage. – Acta bot. sin. 43: 571–577. 2001.Google Scholar
  172. Yang, Q.F., Jiang, H., Xu, D.Q.: Changes in photosynthetic efficiency of flag leaves of wheat during development. – Acta phytophysiol. sin. 25: 408–412, 1999.Google Scholar
  173. Yang, J., Sears, R.G., Gill, B.S., Paulsen, G.M.: Genotypic differences in utilization of assimilate sources during maturation of wheat under chronic heat and heat shock stresses – Utilization of assimilate sources by wheat under heat stresses. – Euphytica 125: 179–188, 2002.Google Scholar
  174. Ying, J., Lee, E.A., Tollenaar, M.: Response of maize leaf photosynthesis to low temperature during the grain-filling period. – Field Crops Res. 68: 87–96, 2002.Google Scholar
  175. Yordanov, I., Georgieva, K., Velikova, V., Tsonev, T., Merakchiiska-Nikolova, M., Paunova, S., Stefanov, D.: Response of the photosynthetic apparatus of different wheat genotypes to drought: I. Laboratory experiments under controlled light and temperature conditions. – Dokl. bolg. Akad. Nauk 54: 79–84, 2001.Google Scholar
  176. Yucel, M., Burke, J.J., Nguyen, H.T.: Inhibition and recovery of photosystem II following exposure of wheat to heat shock. – Environ. exp. Bot. 32: 125–135, 1992.Google Scholar
  177. Zakhidov, E.A., Zakhidova, M.A., Kasymdzhanov, M.A., Kurbanov, S.S., Mirtadzhiev, F.M., Khabibullaev, P.: Chlorophyll fluorescence as a tool for diagnostics of optimal temperatures of photosynthesis in plants. – Dokl. ross. Akad. Nauk 382: 563–566, 2002.Google Scholar
  178. Zhu, X.G., Wang, Q., Zhang, Q.D., Lu, C.M., Kuang, T.V.: Effects of photoinhibition and its recovery on photosynthetic functions of winter wheat under salt stress. – Acta bot. sin. 43: 1250–1254, 2001.Google Scholar
  179. Zidan, I., Azaizeh, H., Newmann, P.M.: Does salinity reduce growth in maize root epidermal cells by inhibiting their capacity for cell wall acidification? – Plant Physiol. 93: 7–11, 1990.Google Scholar

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© Kluwer Academic Publishers 2003

Authors and Affiliations

  • O.H. Sayed
    • 1
  1. 1.Department of Botany, Faculty of ScienceUniversity of MiniaMiniaEgypt

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